4.7 Article

Biochar triggers methanogenesis recovery of a severely acidified anaerobic digestion system via hydrogen-based syntrophic pathway inhibition

期刊

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
卷 46, 期 15, 页码 9666-9677

出版社

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ijhydene.2020.03.115

关键词

Methanogenic recovery; Biochar; Syntrophic VFA degradation; Hydrogen partial pressure; Gibbs free energy; Microbial community analysis

资金

  1. National Natural Science Foundation of China [51978560]
  2. National Key Research and Development Program of China [2017YFE0127300]
  3. Shaanxi Provincial Program for Innovative Research Team [2019TD-025]

向作者/读者索取更多资源

In this study, the use of biochar as an additive was developed to improve the rapid methanogenesis recovery of a severely acidified AD system by enhancing VFA syntrophic oxidation and altering the microbial community structure. The addition of biochar significantly decreased hydrogen partial pressure, thermodynamically stimulating VFA oxidation and enriching electro-active Syntrophomonas and Methanosarcina. This research confirms that biochar is a promising additive for the rapid methanogenic recovery of acidified AD systems.
Rapid methanogenesis recovery of an acidified anaerobic digestion (AD) system is a challenging issue in engineering application, due to the unfavorably thermodynamics of volatile fatty acids (VFA) syntrophic oxidation process with hydrogen as electron transfer mediator. To breakthrough this bottleneck, in this study, we developed the strategy with biochar as an additive for rapid methanogenesis recovery of a severely acidified AD system is elucidated. First, with VFA as substrate, it was found that biochar addition shortened lag time and promoted maximum methane production rate, confirming the role of biochar to enhance VFA syntrophic oxidation. Moreover, the addition of biochar to the acidified sludge significantly decreased hydrogen partial pressure, which thermodynamically stimulated VFA oxidation by reducing Gibbs free energy. Microbial community analysis delivered that biochar addition largely altered the dominant microbes. The enrichment of electro-active Syntrophomonas and Methanosarcina suggested the role of biochar as potential redox-active mediator to stimulate potential direct interspecies electron transfer between syntrophic microorganisms, and inhibit hydrogen-based syntrophic pathway simultaneously. Further stable operation confirms that biochar is a promising additive for the rapid methanogenic recovery of acidified AD systems. (C) 2020 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.

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