期刊
INDUSTRIAL CROPS AND PRODUCTS
卷 149, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.indcrop.2020.112327
关键词
Akebia trifoliata (Thunb.) Koidz flowers; Radio frequency-assisted enzymatic extraction; Plackett-Burman design; Anthocyanin structure; Bioactivity
资金
- Science and Technology Major Project of Yunnan Province [2018ZI001]
- Science and Technology Commission of Shanghai Municipality [19DZ2284200]
Anthocyanins are active compounds with various biological activities and are abundant in Akebia trifoliata (Thunb.) Koidz flowers. To extract anthocyanins from the flowers more effectively, a novel procedure of radio frequency heating-assisted enzymatic extraction was proposed in this study. The extraction conditions were optimized using Plackett-Burman and Box-Behnken design. The optimal extraction conditions were enzyme ratio of cellulase and pectinase of 1:1, enzyme concentration of 1.0 %, ethanol concentration of 50 %, liquid-solid ratio of 50 mL/g, pH of 4, and radio frequency pretreatment at 40 degrees C for 10 min with electrode gap of 5 cm. The results showed that radio frequency assisted enzyme extraction exhibited the highest crude yields (26.55 %) and anthocyanin content (50.87 mg cyanidin-3-O-glucoside equivalents/100 g) compared with hot water, acidified ethanol and enzymatic (pectinase and cellulase) assisted extractions. This method was effective for achieving high 1, 1-diphenyl-2-picrylhydrazyl radical scavenging activity (385.03 Trolox equivalents/g extract), reducing power (361.85 Trolox equivalents/g extract) and tyrosinase inhibitory (14.67 kojic acid equivalents/g extract) activity as well as significant a-amylase inhibitory activity. Nine anthocyanins were identified and did not detect any significant compound degradation using the novel approach. Accordingly, the proposed radio frequency and cellulase/pectinase combined extraction of anthocyanins was efficient and rapid. This method holds great promise for extracting bioactive compounds from plants and fruits.
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