Article
Endocrinology & Metabolism
Qingying Si, Jinxiu Guo, Xiumei Yang, Yujin Guo, Linlin Wu, Dadi Xie, Pei Jiang
Summary: This study investigated the metabolic alterations in streptozotocin-induced diabetic rats using gas chromatography-mass spectrometry analysis. The results showed differential expression of metabolites in the serum, heart, liver, kidney, cortex, renal lipid, hippocampus, and brown fat tissues of diabetic rats compared to control rats. Metabolic pathway analysis revealed that these alterations mainly involved amino acid metabolism, organic acid metabolism, sugar metabolism, and lipid metabolism.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Endocrinology & Metabolism
Yun Huang, Mixue Tu, Yuli Qian, Junyan Ma, Lifen Chen, Yifeng Liu, Yiqing Wu, Kai Chen, Juan Liu, Yanyun Ying, Yao Chen, Yinghui Ye, Lanfeng Xing, Fang Zhang, Yanjun Hu, Runjv Zhang, Ye Chun Ruan, Dan Zhang
Summary: Female fertility declines with age, and the metabolites in follicular fluids are closely related to oocyte quality. This study analyzed the metabolomic profile of follicular fluids from women of different ages and identified metabolites associated with oocyte quality and the outcomes of assisted reproductive technology (ART). These metabolites may serve as potential biomarkers for oocyte maturation and ART outcomes.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zhanxuan E. Wu, Marlena C. Kruger, Garth J. S. Cooper, Ivana R. Sequeira, Anne-Thea McGill, Sally D. Poppitt, Karl Fraser
Summary: Untargeted metabolomics of blood samples has been widely used to study metabolic alterations in diseases and identify biomarkers. This study found that the concentration of metabolites in plasma was more reflective of the liver profile than muscle or adipose tissue, highlighting the importance of considering the metabolomic relationship between different tissues and plasma.
Article
Plant Sciences
Ramona Mihaela Ciubotaru, Pietro Franceschi, Silvia Vezzulli, Luca Zulini, Marco Stefanini, Michael Oberhuber, Peter Robatscher, Giulia Chitarrini, Urska Vrhovsek
Summary: Numerous fungicide applications are needed to control Erysiphe necator, the cause of powdery mildew. This study identifies promising disease-resistant varieties carrying mono-locus or pyramided resistant loci. Metabolic profiling reveals changes in primary compounds, lipids, volatile organic compounds, and phenolic compounds in response to pathogen inoculation. Ten up-accumulated metabolites, including three proposed as resistance biomarkers, distinguish resistant from susceptible varieties in powdery mildew response.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Olga Kiseleva, Ilya Kurbatov, Ekaterina Ilgisonis, Ekaterina Poverennaya
Summary: Metabolomics analyzes metabolites in biological samples, particularly blood and its liquid components: plasma and serum, using advanced analytical chemistry methods. It has been found that small molecules play diverse roles in living systems beyond traditional building blocks or cellular energy fuel, leading to practical applications in molecular medicine and a better understanding of fundamental biochemical processes.
Article
Biochemistry & Molecular Biology
Sing Teang Kong, Hai-Shu Lin, Jianhong Ching, Huiqing Xie, Paul C. Ho
Summary: This study profiled the metabolic changes in rats treated with valproate and found subtle differences in the metabolic changes between males and females. The use of dried blood spots as sample matrices proved to be a powerful tool for monitoring drug levels and metabolic changes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Public, Environmental & Occupational Health
Xuezhi Wang, Zhuhua Wu, Jincheng Zeng, Yuchuan Zhao, Chenchen Zhang, Meiling Yu, Wei Wang, Xunxun Chen, Liang Chen, Jiawen Wang, Liuyue Xu, Jie Zhou, Qiuchan Tan, Wenjing Wei, Yanxia Li
Summary: This study characterized the changes in serum metabolic profiles caused by Mtb infection and standard anti-TB treatment. Seven metabolites were identified to have significantly higher levels in LTBI and untreated PTB patients compared to uninfected healthy controls. Akeboside Ste and sulfolithocholic acid were significantly decreased in PTB patients after treatment. The differential metabolites were mainly involved in glutathione metabolism, ascorbate and aldarate metabolism, and porphyrin and chlorophyll metabolism.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Food Science & Technology
Chengkeng Tan, Jinap Selamat, Nuzul Noorahya Jambari, Rashidah Sukor, Suganya Murugesu, Alfi Khatib
Summary: In this study, an untargeted metabolomics approach using GC-MS and PCA successfully discriminated different chicken breeds, identified characteristic metabolites, and revealed their features. This provides a potential method for distinguishing local village chickens from other breeds.
Article
Endocrinology & Metabolism
Jun Kou, Chunyang He, Lin Cui, Zhengping Zhang, Wei Wang, Li Tan, Da Liu, Wei Zheng, Wei Gu, Ning Xia
Summary: The study on Chinese postmenopausal women with osteoporosis identified potential biomarkers related to metabolic pathway disorders, providing new insights for early diagnosis. The findings suggest that metabonomic analysis has great potential for application in postmenopausal women with osteoporosis.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Food Science & Technology
Dina M. El-Kersh, Soheir E. Kotob, Naglaa M. Ammar, Ola A. M. Mohawed, Hanaa H. Ahmed, Mohamed A. Farag
Summary: This study aimed to identify altered metabolic pathways associated with obesity and discover metabolite biomarkers in the serum of obese rats fed a high fructose diet using metabolomics. The effects of standardized green and black caffeinated extracts (tea and coffee) on obesity and its comorbidities were also investigated. The results showed that treatment with green caffeinated extract, especially in mixture with tea, led to improvements in all parameters. Additionally, the GC-MS analysis identified 34 metabolites, with obese rat serum being enriched in fatty acids (oleamide).
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Simon Lagies, Roman Pichler, Georg Vladimirov, Jana Gawron, Fabian Bazner, Annabell Schreiner, Dajana Kadena, Dietmar A. Plattner, Soeren S. Lienkamp, Bernd Kammerer
Summary: Research has found that the concentration of vancomycin in the kidneys far exceeds plasma levels, potentially leading to nephrotoxic reactions. By studying animal models and cell lines, it was discovered that exposure to vancomycin resulted in metabolic and lipidomic alterations in kidney cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Yanan Li, Dadi Xie, Luxi Li, Pei Jiang
Summary: This study aimed to explore the metabolic changes induced by hypertension at different ages. The results showed that spontaneously hypertensive rats (SHR) at 5 weeks old and 32 weeks old had different metabolic characteristics, mainly involving amino acid metabolism, glycolysis, lipid metabolism, and amino acid metabolism.
CLINICAL AND EXPERIMENTAL HYPERTENSION
(2023)
Article
Biochemical Research Methods
Nerea Lorenzo-Parodi, Erich Leitner, Torsten C. Schmidt
Summary: This study compares three gas chromatographic methods for analyzing ten iodinated derivatives of aromatic amines, and finds that all methods and most analytes have good detection limits and precision. Urine samples from smokers and non-smokers were successfully analyzed, and differences in concentration were observed.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jakub Idkowiak, Grazyna Kubiak-Tomaszewska, Paulina Gatarek, Laukasz Marczak, Joanna Kaluzna-Czaplinska, Wieslaw Tarnowski, Mariusz Uryszek, Barbara Bobrowska-Korczak
Summary: Lesniowski-Crohn's disease is a chronic inflammatory bowel disease that requires surgical intervention to improve quality of life and relieve symptoms. This study found changes in blood metabolome three days after ileo-colic resection, indicating a high energy demand during the post-operative period. These results might contribute to the discussion on perioperative nutrition.
Review
Biochemistry & Molecular Biology
Oliver Gould, Natalia Drabinska, Norman Ratcliffe, Ben de Lacy Costello
Summary: Mass spectrometry (MS) is a versatile analytical technique that is widely used in various scientific fields. It provides new insights into proteomics and metabolomics. MS can detect volatile compounds in human samples for disease diagnosis, drug monitoring, and metabolic processes. Hyphenated MS techniques offer qualitative analysis with high accuracy and sensitivity, while real-time MS techniques provide highly sensitive quantitative data in a shorter time frame.
Article
Immunology
Paolo Corradini, Chiara Agrati, Giovanni Apolone, Alberto Mantovani, Diana Giannarelli, Vincenzo Marasco, Veronica Bordoni, Alessandra Sacchi, Giulia Matusali, Carlo Salvarani, Pier Luigi Zinzani, Renato Mantegazza, Fabrizio Tagliavini, Maria Teresa Lupo-Stanghellini, Fabio Ciceri, Silvia Damian, Antonio Uccelli, Daniela Fenoglio, Nicola Silvestris, Fausto Baldanti, Giulia Piaggio, Gennaro Ciliberto, Aldo Morrone, Franco Locatelli, Valentina Sinno, Maria Rescigno, Massimo Costantini
Summary: This study investigated the immune response to the SARS-CoV-2 vaccine in patients with tumors and immune disorders. The results showed that immunosuppressive treatment was a risk factor for a low humoral response, while the booster dose improved both humoral and T-cell responses.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Rheumatology
Sara Monti, Chiara Fornara, Paolo Delvino, Alice Bartoletti, Federica Bergami, Giuditta Comolli, Jose Camilla Sammartino, Alessandro Biglia, Emanuele Bozzalla Cassione, Irene Cassaniti, Fausto Baldanti, Daniele Lilleri, Carlomaurizio Montecucco
Summary: This study analyzed the immune response to mRNA COVID-19 vaccines in patients with GCA. The results showed that GCA patients had weaker antibody response and impaired neutralizing activity compared to healthy individuals. Treatment with MTX significantly reduced the humoral response. About one-third of GCA patients did not develop cellular immune protection in response to the COVID-19 vaccine.
Article
Virology
Federica Zavaglio, Francesca Rivela, Irene Cassaniti, Francesca Arena, Elisa Gabanti, Anna L. Asti, Daniele Lilleri, Teresa Rampino, Fausto Baldanti, Marilena Gregorini
Summary: Measuring the amount of CMV-specific T cells can help identify solid organ transplant recipients who are protected against CMV disease. This study used flow cytometry and ELISPOT assays to measure T cell frequencies. CD4(+) CFC-iDC and ELISPOT-iCL had better diagnostic performance than CD8(+) CFC-iDC, and provided earlier detection of protective immune reconstitution. This monitoring method is effective in discriminating transplant recipients at risk of or protected from CMV disease.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Letter
Dermatology
Paola Zelini, Eugenio Isoletta, Martina Volonte, Daniele Lilleri, Irene Cassaniti, Valeria Musella, Catherine Klersy, Fausto Baldanti, Valeria Brazzelli
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2023)
Article
Virology
Cristina Galli, Laura Pellegrinelli, Federica Giardina, Guglielmo Ferrari, Sara Colonia Uceda Renteria, Federica Novazzi, Elisa Masi, Elisabetta Pagani, Giulia Piccirilli, Maria Vittoria Mauro, Sandro Binda, Benedetta Corvaro, Claudia Tiberio, Eleonora Lalle, Fabrizio Maggi, Cristina Russo, Stefania Ranno, Elisa Vian, Elena Pariani, Fausto Baldanti, Antonio Piralla
Summary: This study aimed to assess the circulation and molecular evolution of influenza viruses (IVs), particularly A(H3N2), during the 2021-2022 season in Italy. Analysis of respiratory specimens collected from January 1, 2022 to May 31, 2022 showed that all confirmed IVs were type A, with a majority being H3N2. Phylogenetic analysis revealed genetic clusters and mutations in the HA gene sequences, suggesting the presence of drifted strains. The study highlights the importance of molecular surveillance in assessing vaccine effectiveness and detecting novel strains.
Article
Virology
Salvatore Camiolo, Joseph Hughes, Fausto Baldanti, Milena Furione, Daniele Lilleri, Giuseppina Lombardi, Micol Angelini, Giuseppe Gerna, Maurizio Zavattoni, Andrew J. Davison, Nicolas M. Suarez
Summary: Understanding the intrahost evolution of viral populations is important for pathogenesis, diagnosis, and treatment. However, the analysis is sensitive to bias, error, and artefact in the data, and it is crucial to address these issues adequately.
Article
Infectious Diseases
Cristina Merla, Irene Mileto, Stefano Gaiarsa, Cristian Achille, Stefano Ghirardello, Marta Corbella, Fausto Baldanti, Patrizia Cambieri
Summary: We report the isolation of a strain of Pantoea genus carrying a VIM carbapenemase, which is the first of its kind. The strain was obtained from a rectal swab of a 10-day-old newborn in a neonatal intensive care unit (NICU). Whole-genome sequencing revealed that the strain was identified as Pantoea brenneri. The strain harbored the carbapenemase gene bla(VIM-1). Immediate contact measures and isolation of the newborn prevented the dissemination of VIM-producing P. brenneri and the plasmid carrying the VIM-1 gene to other newborns.
Article
Genetics & Heredity
Daniela Matuozzo, Estelle Talouarn, Astrid Marchal, Peng Zhang, Jeremy Manry, Yoann Seeleuthner, Yu Zhang, Alexandre Bolze, Matthieu Chaldebas, Baptiste Milisavljevic, Adrian Gervais, Paul Bastard, Takaki Asano, Lucy Bizien, Federica Barzaghi, Hassan Abolhassani, Ahmad Abou Tayoun, Alessandro Aiuti, Ilad Alavi Darazam, Luis M. Allende, Rebeca Alonso-Arias, Andres Augusto Arias, Gokhan Aytekin, Peter Bergman, Simone Bondesan, Yenan T. Bryceson, Ingrid G. Bustos, Oscar Cabrera-Marante, Sheila Carcel, Paola Carrera, Giorgio Casari, Khalil Chaibi, Roger Colobran, Antonio Condino-Neto, Laura E. Covill, Ottavia M. Delmonte, Loubna El Zein, Carlos Flores, Peter K. Gregersen, Marta Gut, Filomeen Haerynck, Rabih Halwani, Selda Hancerli, Lennart Hammarstroem, Nevin Hatipoglu, Adem Karbuz, Sevgi Keles, Christele Kyheng, Rafael Leon-Lopez, Jose Luis Franco, Davood Mansouri, Javier Martinez-Picado, Ozge Metin Akcan, Isabelle Migeotte, Pierre-Emmanuel Morange, Guillaume Morelle, Andrea Martin-Nalda, Giuseppe Novelli, Antonio Novelli, Tayfun Ozcelik, Figen Palabiyik, Qiang Pan-Hammarstroem, Rebeca Perez de Diego, Laura Planas-Serra, Daniel E. Pleguezuelo, Carolina Prando, Aurora Pujol, Luis Felipe Reyes, Jacques G. Riviere, Carlos Rodriguez-Gallego, Julian Rojas, Patrizia Rovere-Querini, Agatha Schlueter, Mohammad Shahrooei, Ali Sobh, Pere Soler-Palacin, Yacine Tandjaoui-Lambiotte, Imran Tipu, Cristina Tresoldi, Jesus Troya, Diederik van de Beek, Mayana Zatz, Pawel Zawadzki, Saleh Zaid Al-Muhsen, Mohammed Faraj Alosaimi, Fahad M. Alsohime, Hagit Baris-Feldman, Manish J. Butte, Stefan N. Constantinescu, Megan A. Cooper, Clifton L. Dalgard, Jacques Fellay, James R. Heath, Yu-Lung Lau, Richard P. Lifton, Tom Maniatis, Trine H. Mogensen, Horst von Bernuth, Alban Lermine, Michel Vidaud, Anne Boland, Jean-Francois Deleuze, Robert Nussbaum, Amanda Kahn-Kirby, France Mentre, Sarah Tubiana, Guy Gorochov, Florence Tubach, Pierre Hausfater, C. O. V. I. D. Human Genetic Effort, Isabelle Meyts, Shen-Ying Zhang, Anne Puel, Luigi D. Notarangelo, Stephanie Boisson-Dupuis, Helen C. Su, Bertrand Boisson, Emmanuelle Jouanguy, Jean-Laurent Casanova, Qian Zhang, Laurent Abel, Aurelie Cobat
Summary: Through a genome-wide rare variant burden association analysis, it was found that there is an association between at-risk variants in the TLR7 gene and rare loss-of-function variants in TLR3-dependent type I interferon immunity genes. These findings suggest that rare variants in TLR3- and TLR7-dependent type I interferon immunity genes may underlie life-threatening COVID-19 in patients under 60 years old.
Article
Public, Environmental & Occupational Health
Jose Camilla Sammartino, Marta Colaneri, Cecilia Bassoli, Mariaelena Ceresini, Antonio Piralla, Alessandro Ferrari, Elena Percivalle, Fausto Baldanti, Raffaele Bruno, Mario U. Mondelli
Summary: This study aimed to verify the presence of viable virus on different surfaces exposed to droplets released by coughing in SARS-CoV-2 RNA positive patients. The results showed that droplets on common inanimate surfaces did not contain viable virus, while laboratory-propagated viruses could survive and grow on the same surfaces.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2023)
Article
Immunology
Marta Colaneri, Raffaella Lissandrin, Matteo Calia, Cecilia Bassoli, Elena Seminari, Alessandro Pavesi, Francesca Rovida, Fausto Baldanti, Alba Muzzi, Guido Chichino, Angelo Regazzetti, Cecilia Grecchi, Angelo Pan, Matteo Lupi, Erica Franceschini, Cristina Mussini, Raffaele Bruno
Summary: This study aimed to assess the efficacy of steroid therapy in reducing intrahospital mortality, length of stay, and neurological sequelae in WNND patients. The results showed that steroid therapy did not significantly reduce intrahospital mortality or neurological sequelae. More prospective data are needed to demonstrate the protective effect of steroid treatment.
OPEN FORUM INFECTIOUS DISEASES
(2023)
Article
Microbiology
Eun Kim, Muhammad S. Khan, Alessandro Ferrari, Shaohua Huang, Jose C. Sammartino, Elena Percivalle, Thomas W. Kenniston, Irene Cassaniti, Fausto Baldanti, Andrea Gambotto
Summary: The study evaluates the booster effect of an S1 subunit vaccine in aged mice previously primed with adenoviral vaccines. It demonstrates that a booster dose of the rS1Beta subunit vaccine, given to mice, induces strong and long-lived S1-specific immune responses and significantly increases neutralizing antibodies against Wuhan, Beta, and Delta strains, including cross-reactive antibodies against Omicron variants. The findings suggest that the rS1Beta subunit vaccine can offer cross-neutralization against broad variants, providing important implications for controlling breakthrough SARS-CoV-2 variants in elderly individuals primed with adenovirus-based vaccines.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Valentina Zuccaro, Greta Petazzoni, Irene Mileto, Marta Corbella, Erika Asperges, Paolo Sacchi, Sara Rattotti, Marzia Varettoni, Irene Defrancesco, Patrizia Cambieri, Fausto Baldanti, Luca Arcaini, Raffaele Bruno
Summary: Several studies have shown a strong connection between gut microbiota and the response to immunotherapy in tumor patients, suggesting that gut microbiota can serve as a biomarker for response. This study aimed to compare the gut microbiota diversity in chronic lymphocytic leukemia patients treated with B-cell receptor inhibitors for at least 12 months. The findings revealed differences in bacterial distribution between different response groups.
Article
Microbiology
Paola Zelini, Piera d'Angelo, Federica Zavaglio, Ehsan Soleymaninejadian, Liliana Mariani, Francesca Perotti, Mattia Dominoni, Stelvio Tonello, Pierpaolo Sainaghi, Rosalba Minisini, Daria Apostolo, Daniele Lilleri, Arsenio Spinillo, Fausto Baldanti
Summary: In this prospective study, it was found that vaccinated pregnant women and their newborns had higher levels of cytokines and immune responses after SARS-CoV-2 infection. Additionally, the newborns of vaccinated mothers had higher levels of specific antibodies. Vaccination can enhance the immune profile of pregnant women and provide immune protection to newborns.
Article
Immunology
Simona Secondino, Paolo Pedrazzoli, Sabrina Basso, Paolo Bossi, Alba Bianco, Ilaria Imarisio, Anna Pagani, Marica De Cicco, Stella Muscianisi, Michela Casanova, Carlo Morosi, Cristiana Bergamini, Marco Benazzo, Maria Cossu Rocca, Cesare Perotti, Fausto Baldanti, Marco Zecca, Lisa F. Licitra, Patrizia Comoli
Summary: This study aimed to explore the efficacy of autologous EBV-specific CTL transplantation in the treatment of recurrent/metastatic EBV-NPC patients after chemotherapy. The results showed that patients who received CTL transplantation had a 5-year overall survival rate of 44% and a median overall survival of 60 months. This indicates that autologous CTL transplantation can effectively control the disease.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Angioletta Lasagna, Irene Cassaniti, Francesca Arena, Federica Bergami, Elena Percivalle, Giuditta Comolli, Antonella Sarasini, Alessandro Ferrari, Daniela Cicognini, Roberta Schiavo, Giuliana Lo Cascio, Paolo Pedrazzoli, Fausto Baldanti
Summary: We prospectively evaluated the long-term immunogenicity of the third dose of the SARS-CoV-2 BNT162b2 mRNA vaccine in 55 patients undergoing cancer treatment. Our findings indicate that one year after receiving the third dose, more than 67% of patients showed an immune response against the virus, with less than 20% of patients showing no response to certain variants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medical Laboratory Technology
Jiansheng Lin, Weihua Lin, Yiming Lin, Weilin Peng, Zhenzhu Zheng
Summary: This study retrospectively analyzed the cases of Chinese infants with NICCD and identified multiple genetic mutations. The study also found comorbidity of NICCD and other inborn errors of metabolism in some patients.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Lihua Guan, Wei Su, Jian Zhong, Ling Qiu
Summary: Multiple myeloma is characterized by excessive production of monoclonal immunoglobulins. Routine screening methods are insufficient for detecting low levels of M proteins, but advances in mass spectrometry enable reliable detection of low abundance serum biomarkers for minimal residual disease assessment in multiple myeloma.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Liya Zhu, Chao Zhu, Jialie Jin, Jinxin Wang, Xiaojing Zhao, Rongxi Yang
Summary: This study found an association between blood-based ITGB2 methylation and coronary heart disease (CHD), with hypomethylation of ITGB2 being a risk factor for CHD. Additionally, the combination of ITGB2 methylation and conventional CHD risk factors could efficiently discriminate CHD patients from controls.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
H. Al Habobe, E. B. Haverkort, K. Nazmi, A. P. Van Splunter, R. H. H. Pieters, F. J. Bikker
Summary: Saliva diagnostics have become popular due to their non-invasive nature and patient-friendly collection process. However, the choice of saliva collection method can affect the measured levels of various biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Abdurrahman Coskun, Anna Carobene, Ozlem Demirelce, Michele Mussap, Federica Braga, Ebru Sezer, Aasne Karine Aarsand, Sverre Sandberg, Pilar Fernandez Calle, Jorge Diaz-Garzon, Metincan Erkaya, Cihan Coskun, Esila Nur Erol, Hunkar Dag, Bill Bartlett, Mustafa Serteser, Niels Jonker, Ibrahim Unsal
Summary: In this study, BV estimates for 22 AAs were provided based on a large sample size, and it was found that there are differences in CVI estimates between males and females for most AAs, which has implications for the clinical interpretation and use of AAs.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Valentinus Besin, Farizky Martriano Humardani, Trilis Yulianti, Matthew Justyn
Summary: The study of Parkinson's Disease in Asian populations has revealed the impact of genetic variants on multiple biological pathways and highlighted shared genetic susceptibility with other diseases. These findings emphasize the importance of personalized treatment based on individual genetic profiles.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Simona Ferraro, Sara Benedetti, Savina Mannarino, Santica Marcovina, Elia Mario Biganzoli, Gianvincenzo Zuccotti
Summary: Risk stratification for cardio-vascular disease should be implemented in childhood to promote early prevention strategies, as atherosclerotic lesions can be present even in very young individuals. Evaluating pediatric CV risk factors/clinical conditions and conducting lipid profile and genetic testing can help identify children at risk of future CV events and guide appropriate therapeutic options.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Ahmad Mobed, Bita Abdi, Sajjad Masoumi, Mohammad Mikaeili, Elham Shaterian, Hamed Shaterian, Esmat Sadat Kazemi, Mahdiye Shirafkan
Summary: Reproductive biomarkers play important regulatory roles in women. The discovery and quantification of these biomarkers are clinically significant. Various detection strategies, including nanotechnology-based methods, have been developed. This article provides an in-depth introduction to the latest advances in biosensor and nanosensor research for detecting and quantitatively identifying reproductive biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Chengfang Tang, Fang Tang, Yanna Cai, Minyi Tan, Sichi Liu, Ting Xie, Xiang Jiang, Yonglan Huang
Summary: This study proposes an effective method for screening X-ALD and evaluates the performance of newborn screening for X-ALD in Guangzhou. The LC-MS/MS method can accurately identify X-ALD through analysis of C26:0-LPC.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Lan Liu, Jiamin Wang, Xijing Liu, Jing Wang, Lin Chen, Hongmei Zhu, Jingqun Mai, Ting Hu, Shanling Liu
Summary: The 16p11.2 deletion is a common genetic cause of neurodevelopmental disorders, with prenatal and postnatal presentations including vertebral malformations and language impairment. The majority of deletions are de novo and may be associated with MAPK3 and histidine-associated metabolism.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Sneha Venkatesalu, Shanmugapriya Dilliyappan, Avanthika Satish Kumar, Thirunavukkarasu Palaniyandi, Gomathy Baskar, Maddaly Ravi, Asha Sivaji
Summary: Microfluidics is a science and technology that deals with less sample-to-more precision in vitro analysis. It has wide applications in cancer theranostics, enabling precise diagnosis and personalized treatment.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Yuanqin Zhao, Wei Deng, Zhaoyue Wang, Yanxia Wang, Hongyu Zheng, Kun Zhou, Qian Xu, Le Bai, Huiting Liu, Zhong Ren, Zhisheng Jiang
Summary: The cardiovascular system and the central nervous system exhibit a coordinated developmental process during embryonic development. Congenital heart disease (CHD) is the most common congenital disorder, and neurodevelopmental disorders (NDD) are common complications in CHD patients. Both genetic and non-genetic factors contribute to the co-occurrence of CHD and NDD. Further research should focus on identifying common molecular mechanisms underlying this co-occurrence and promoting the research and treatment of developmental disorders related to the cardiovascular and central nervous systems.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Karol Gostomczyk, Ewelina Lukaszewska, Jedrzej Borowczak, Anita Bator, Marek Zdrenka, Magdalena Bodnar, Lukasz Szylberg
Summary: Flow cytometry improves the detection of epithelial cancer cells in peritoneal and pleural fluids compared to conventional cytology. Due to similar speciflcity and higher sensitivity, flow cytometry offers a promising alternative to cytology for patient screening.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Heping Tian, Genghuan Wang, Qi Zhong, Haihang Zhou
Summary: This study found that the decline of serum ITIH4 concentrations during the early phase after aneurysmal subarachnoid hemorrhage (aSAH) was closely related to the severity and poor prognosis of the disease. Serum ITIH4 may represent a promising prognostic biomarker of aSAH.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Xueting Zhu, Yang Yu, Jun Zhang, Yuxia Zhan, Guanghua Luo, Lu Zheng
Summary: This study successfully established a 2D-PCR method for identifying HLA-B*15:02 through a two-tube reaction. This method can distinguish HLA-B*15:02 from 16 highly homologous HLA-B*15 alleles. Among 1830 samples from the clinical general population, 3 HLA-B*15:02 homozygotes and 84 HLA-B*15:02 heterozygotes were detected.
CLINICA CHIMICA ACTA
(2024)