期刊
CLINICA CHIMICA ACTA
卷 463, 期 -, 页码 11-17出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.cca.2016.10.010
关键词
Lupus; ITK; Th17; Treg
资金
- National Natural Science Foundation of China [30901340, 30972746]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20120181110009]
Background: Interleukin-2 inducible T-cell kinase (ITK) is expressed in T cells, and plays an important role in autoimmune inflammatory diseases through regulating the balance of Th17/Treg. However, its role in human systemic lupus erythematosus (SLE) remains unclear. The present study aims to measure the activation status of ITK in T cells from SLE patients and healthy controls, and identify its possible correlation to disease severity. We also discuss the serum levels of Th17, Treg related cytokines including IL-17, IL-21, IL-22, IL-10, analyzing correlation between ITK and Th17/Freg related cytokines. Methods: Peripheral blood samples were drawn from 42 patients with SLE and 43 healthy blood donors, and the phosphorylation of ITK protein was studied in T cells using flow cytometry. In addition, serum levels of Th17/Freg related cytokines were studied with enzyme-linked immunosorbent assay (ELISA). Results: Percentages of CD4(+) pITK(+) T cells, CD8(+) pITK(+) T cells were higher in SLE patients compared with controls, and were positively related to disease activity, some clinical and laboratory parameters. Percentages of CD4(+) pITK(+) T cells, CD8(+)pITK(+) T cells were more prominent in active SLE patients compared with less active patients. Serum levels of Th17 and Treg related cytokines were higher in patients compared with controls. CD4(+)pITK(+) T cells were related to levels of IL-17, IL-21. Conclusion: These data indicate that increased ITK expression could act as a disease activity marker and as a risk factor for involvement in SLE, but it still needs further study to confirm. (C) 2016 Elsevier B.V. All rights reserved.
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