期刊
CLINICA CHIMICA ACTA
卷 459, 期 -, 页码 63-72出版社
ELSEVIER
DOI: 10.1016/j.cca.2016.05.026
关键词
Gestational diabetes mellitus; Bile acid; Serum; Metabolic profiling; Biomarker; Ultrahigh performance liquid chromatography/hybrid quadrupole time-of-flight mass spectrometry
资金
- National Natural Science Foundation of China [21403021]
- Postdoctoral Science Foundation of Chongqing [Xm201313]
- Fundamental and Advanced Research Foundation of Chongqing Science and Technology Commission [cstc2013jcyjA20004]
- Scientific Research Foundation of Chongqing Municipal Education Commission [KJ130314]
- Doctoral Program Science Foundation of Higher Education of China [20115503110013]
Background: Given the potential influence of aberrant bile acid metabolism on glucose homeostasis, we hypothesized that serum bile acid metabolism is altered in gestational diabetes mellitus (GDM). We characterized the metabolic profiling changes of serum bile acids in GDM and to find the potential biomarkers for the diagnosis and differential diagnosis of GDM. Methods: Based on ultrahigh performance liquid chromatography/hybrid quadrupole time-of-flight mass spectrometry, a targeted metabolomics study that involved targeted and untargeted screening techniques was performed to explore the changes in serum bile acid metabolism of GDM cases, intrahepatic cholestasis of pregnancy (ICP) cases and healthy controls. Results: There were 3 significantly different profiling of serum bile acids for GDM, ICP and controls. Compared to the controls, GDM individuals demonstrated significant increases in 8 bile acid species, including 2 dihydroxy conjugated, 1 trihydroxy unconjugated and 5 sulfated bile acids. beta-muricholic acid (beta-MCA) and di-2 were well-suited to use as the metabolic markers for the diagnosis and differential diagnosis of GDM, respectively. Conclusions: These preliminary findings revealed the protective effect of body against cytotoxicity via elimination of increased sulfated bile acids and aberrant enzyme activity participated in the cycle beta-MCA -> hyodeoxycholic acid (HDCA) of the bile acid metabolism pathway for the women with GDM, which gave us further insights into the etiology and pathophysiology of GDM. (C) 2016 Elsevier B.V. All rights reserved.
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