期刊
FOOD CHEMISTRY
卷 317, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.126361
关键词
Detection; Nitrite; Glucosamine hydrochloride; Colorimetric method
资金
- National Natural Science Foundation of China [21305097]
- General Project of Education Department in Sichan [035Z2270]
- Two-Way Support Programs of Sichuan Agricultural University [00770113]
A rapid, sensitive, and highly selective method for determining nitrite in food has been developed. This method is based on the reaction of nitrite with the amino group of 3,3,5,5-tetramethylbenzidine (TMB) to form a diazonium salt, and then the diazonium salt and glucosamine hydrochloride are coupled to each other to form an orange compound. The optimal conditions for maximum color and other analytical parameters were studied. A colorimetric method for nitrite detection has been developed with an outstanding correlation coefficient (R-2 = 0.9944), a wide linear range (1-75 mu M) and 0.73 mu M limit of detection (at S/N = 3) for nitrite ions. This method was successfully applied to the determination of nitrite in a variety of foods and gave recoveries in the range between 100.16% and 103.07%, demonstrating that the accuracy, reliability and potential application of this assay for monitoring nitrite in foods.
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