期刊
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
卷 27, 期 34, 页码 42969-42978出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s11356-020-10192-6
关键词
FusariumZH-H2; HMW-PAHs; Biodegradation; Ligninolytic system; Laccase; Lignin peroxidase
资金
- Key R&D Program in Hebei Province [19223811D]
- National key R&D Program of China [2018YFD0800402]
- Educational Commission of Hebei Province of China [Z2013058]
- National High-Tech R&D Program of China (863 Program) [2012AA101403]
The capacity ofFusariumsp. strain ZH-H2 to secret lignin peroxidase (LiP), laccase (Lac), and manganese peroxidase (MnP) and degrade high molecular weight polycyclic aromatic hydrocarbons (HMW-PAHs) was studied. When the fungus was grown in control mineral salt medium for 4 days, LiP and Lac activities were detected at 8871 U L(-1)and 5123 U L-1, respectively. In the presence of HMW-PAHs as the sole carbon source, only LiP activity was detectable, and LiP activity had significantly reduced HMW-PAHs at day 7, with a maximum decrease of 85.9%. A strong correlation between LiP activity and HMW-PAHs removal efficiency could be fit into various models, with the highest correlation coefficients obtained for quadratic functions (P < 0.01). When a specific enzyme inhibitor was added, the ability ofFusariumto remove HMW-PAHs was reduced from 85.9 to 66.7%, depending on the inhibitor's concentration. Meanwhile, the determined activity of LiP was reduced from 11.4 to 48.6%. We conclude that in the presence of HMW-PAHs as the only carbon source to support growth,FusariumZH-H2 mainly produces LiP but not Lac or MnP for HMW-PAHs degradation. To our knowledge, it was the first time to propose a metabolic lignin peroxidase characterization of HMW-PAHs degradation byFusariumsp. strains.
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