Article
Biochemistry & Molecular Biology
Chao Dong, Liwei An, Cheng-han Yu, Michael S. Y. Huen
Summary: The DYRK1B kinase plays a crucial role in maintaining rDNA stability, nucleolar reorganization, and inhibition of rRNA synthesis. Inhibition of DYRK1B results in sustained nucleolar transcription, hypersensitivity to DSBs at rDNA arrays, and requirement for DSB repair and rDNA copy number maintenance. These findings highlight the importance of DYRK1B as a key signaling intermediate in coordinating DSB repair and rDNA transcriptional activities within the nucleolus.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
C. Fritsch, J-F Gout, S. Haroon, A. Towheed, C. Chung, J. LaGosh, E. McGann, X. Zhang, Y. Song, S. Simpson, P. S. Danthi, B. A. Benayoun, D. Wallace, K. Thomas, M. Lynch, M. Vermulst
Summary: Research shows that mutagenic compounds not only cause genetic mutations, but are also a powerful source of transcription errors. These errors occur in both dividing and nondividing cells, and sometimes greatly exceed the number of mutations in the genome. Additionally, DNA repair is crucial in mitigating transcriptional mutagenesis after exposure to mutagens.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Zhihui Liu, Michael J. Kruhlak, Carol J. Thiele
Summary: Zinc finger transcription factor CASZ1b is essential for nervous system development and suppresses neuroblastoma growth. Our study investigates the recruitment of CASZ1b to sites of DNA damage, showing that CASZ1b is transiently recruited to these sites and that its recruitment is dependent on PARP and specific DNA binding motifs. Loss of CASZ1b increases cell sensitivity to DNA damage induced by gamma irradiation and suggests a role for CASZ1b in regulating DNA repair efficiency during development and tumorigenesis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Jung Min Kim
Summary: Microtubules are key components of the cytoskeleton and play important roles in cellular processes and genome maintenance. This review highlights recent advances in understanding the role of microtubule dynamics in DNA damage response and repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Colin J. Dinsmore, Philippe Soriano
Summary: Serum response factor (SRF) is a crucial transcription factor that regulates various cellular processes. It controls the expression of immediate early genes and actin cytoskeleton-related genes, which are involved in cell proliferation, migration, and differentiation. SRF coordinates these processes through the interaction with its cofactors, ternary complex factors (TCFs) and myocardin-related transcription factors (MRTFs). This study demonstrates the important role of MRTFs in mediating SRF function during cardiac neural crest development, providing new insights into the transcriptional regulation mechanism of SRF during development.
Article
Chemistry, Multidisciplinary
William W. Du, Javeria Qadir, Kevin Y. Du, Yu Chen, Nan Wu, Burton B. Yang
Summary: Current studies have shown that nuclear actin plays a significant role in the regulation of epithelial-mesenchymal transition (EMT). Different binding partners for nuclear F-actin and G-actin have been discovered, which respectively modulate EMT-promoting and EMT-repressing transcriptional events. Mechanistically, nuclear F-actin enhances the expression and stability of proteins involved in EMT promotion, while nuclear G-actin increases the expression and stability of proteins involved in EMT repression. The association between nuclear actin and EMT suggests that targeting nuclear actin dynamics for dysregulated EMT is a promising area for further study.
Article
Cardiac & Cardiovascular Systems
Leila Rouhi, Sirisha M. Cheedipudi, Suet Nee Chen, Siyang Fan, Raffaella Lombardi, Xiaofan Chen, Cristian Coarfa, Matthew J. Robertson, Priyatansh Gurha, Ali J. Marian
Summary: The study identified that TMEM43 haploinsufficiency leads to activation of the DDR and TP53 pathways, resulting in increased expression of SASP and an age-dependent pro-fibrotic cardiomyopathy. The shared mechanism in the pathogenesis of cardiomyopathies caused by mutations involving nuclear envelope proteins may involve DNA damage response.
CARDIOVASCULAR RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Hadar Golan Berman, Pooja Chauhan, Shira Shalev, Hiba Hassanain, Avital Parnas, Sheera Adar
Summary: The study identified that in a specific cell line system, cisplatin resistant cells had less damage but similar repair kinetics. Through mapping gene expression changes, 56 upregulated genes were identified that could potentially impact the response to cisplatin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Ekaterina Sidorenko, Maria Sokolova, Antti P. Pennanen, Salla Kyheroinen, Guido Posern, Roland Foisner, Maria K. Vartiainen
Summary: This study reveals a novel mechanism for regulating MRTF-A activity. It shows that lamina-associated polypeptide 2 alpha (Lap2 alpha) is a direct binding partner of MRTF-A and is required for efficient expression of MRTF-A/SRF target genes.
SCIENTIFIC REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Alexander W. Cook, Christopher P. Toseland
Summary: Myosins within the nucleus play crucial roles in cell function and survival, including transcription, DNA damage, and viral replication. They act as molecular transporters and anchors, relying on their actin binding and ATPase capabilities. Their roles in the DNA damage response vary from transcriptional responses to moving chromatin and stabilizing chromosome contacts.
JOURNAL OF BIOCHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Mateusz Kciuk, Adrianna Gielecinska, Damian Kolat, Zaneta Kaluzinska, Renata Kontek
Summary: Transcription factors play important roles in oncogenesis, tumor progression, and metastasis, and recent studies have found their involvement in the DNA damage response pathway. This review focuses on two transcription factors, HIF1 alpha and MYC, with dual roles in oncogenesis and metastasis, as well as three EMT transcription factors, TWIST, ZEB1, and ZNF281, associated with the control of canonical DDR pathways.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2022)
Article
Biochemistry & Molecular Biology
Qichao Huang, Dan Wu, Jing Zhao, Zeyu Yan, Lin Chen, Shanshan Guo, Dalin Wang, Chong Yuan, Yinping Wang, Xiaoli Liu, Jinliang Xing
Summary: This study reveals the importance of TFAM in cancer metastasis, showing that TFAM deficiency induces nuclear actin polymerization and enhances the metastatic ability of HCC cells. The deficiency of TFAM blocks the TCA cycle and increases intracellular malonyl-CoA levels, promoting the nuclear translocation of actin and facilitating HCC cell metastasis.
Review
Cell Biology
Yu-Hsiu Wang, Michael P. Sheetz
Summary: The mechanisms that maintain genome stability are crucial for preventing tumor progression. Nuclear phosphoinositide lipids play an important role in DNA damage signaling and provide a new signaling interface for DNA repair pathway selection. However, our understanding of the underlying regulatory mechanisms of nuclear phosphoinositides in DNA damage repair is limited due to a lack of techniques for real-time monitoring of changes in these lipids.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cardiac & Cardiovascular Systems
Lin Wu, James R. Sowers, Yingmei Zhang, Jun Ren
Summary: Cardiovascular diseases (CVDs) are caused by abnormalities in the genomic, proteomic, and metabolomic aspects. Recent evidence has confirmed the presence of DNA damage in various CVDs. DNA damage triggers a cellular response called DNA damage response (DDR), which can be beneficial for cardiovascular function temporarily, but persistent activation of DDR promotes the onset and development of CVDs. Therefore, targeting DNA damage and DDR could potentially improve cardiovascular dysfunction and disease outcome.
CARDIOVASCULAR RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Marina Francis, Anis Ahmad, Larry Bodgi, Patrick Azzam, Tarek Youssef, Alaa Abou Daher, Assaad A. Eid, Alessia Fornoni, Alan Pollack, Brian Marples, Youssef H. Zeidan
Summary: This study reveals a novel role for SMPDL3b in the radiation-induced DNA damage response, including modulation of nuclear sphingolipid metabolism, ATM nuclear shuttling, and DSBs repair. SMPDL3b overexpression enhances DSBs recognition and repair, and protects renal podocytes against radiation-induced apoptosis.
