Article
Microbiology
Jiyuan Chen, Dansha Zhou, Jinrui Miao, Chenting Zhang, Xiang Li, Huazhuo Feng, Yue Xing, Zizhou Zhang, Changlei Bao, Ziying Lin, Yuqin Chen, Jason X. -J. Yuan, Dejun Sun, Kai Yang, Jian Wang
Summary: This study investigated the association between gut microbiota composition and host metabolome signatures in a rat model of pulmonary hypertension induced by left pulmonary artery ligation (LPAL). The results showed significant dysbiosis of the gut microbiota in LPAL-PH rats, along with specific changes in gut and lung metabolome profiles. These findings contribute to our understanding of the gut microbiome and its connection to lung health in LPAL-PH, providing potential new directions for therapy and management.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
W. Xu, S. Mann, G. Curone, A. Kenez
Summary: The study examined the effects of heat treatment on colostrum metabolome and the serum metabolome of newborn calves fed heat-treated colostrum. It was found that heat treatment influenced the colostrum metabolome, but did not have a significant impact on the serum metabolome of calves. Further studies with larger sample sizes and complementary analytical techniques are needed to explore potential alterations in colostrum metabolome induced by heat treatment.
Article
Pharmacology & Pharmacy
Yang-Yang He, Xin-Mei Xie, Hong-Da Zhang, Jue Ye, Selin Gencer, Emiel P. C. van der Vorst, Yvonne Doering, Christian Weber, Xiao-Bin Pang, Zhi-Cheng Jing, Yi Yan, Zhi-Yan Han
Summary: This study identified altered metabolites and unique metabolism associated genes (MAGs) in rat PASMCs under hypoxia, as well as differentially expressed MAGs in mouse lungs of hypoxic PH. Furthermore, six hub genes induced by hypoxia were revealed, showing potential as therapeutic targets for hypoxic PH.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Plant Sciences
Atsushi Fukushima, Mikiko Takahashi, Hideki Nagasaki, Yusuke Aono, Makoto Kobayashi, Miyako Kusano, Kazuki Saito, Norio Kobayashi, Masanori Arita
Summary: The advancement of metabolomics has enabled rapid detection and quantification of cellular metabolites. However, shortcomings in data sharing still exist. To address these issues, RIKEN developed the RIKEN PMM, a database that stores plant metabolomic data and experimental metadata.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Engineering, Environmental
Yuxin Qiao, Nan Zhang, Jintong Liu, Hong Yang
Summary: Agrochemicals such as pesticide residues pose ecotoxic risks to plant, animal, and human health. This study investigated the toxicology and degradation mechanisms of the widely used pesticide ametryn (AME) in rice crops. AME was found to be absorbed by rice roots, causing repression of plant elongation and reduction in dry weight and chlorophyll concentration. Transcriptome and metabolome analysis revealed AME-responsive genes and step-wise catabolism of AME in rice. The study also identified AME metabolites and conjugates that are closely related to differentially expressed genes, differentially accumulated metabolites, and activities of antioxidative enzymes. These findings contribute to the development of genetically engineered rice for bioremediation of AME-contaminated soils and reduction of AME accumulation in rice crops.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Pharmacology & Pharmacy
Marie Gasser, Sebastien Lenglet, Nasim Bararpour, Tatjana Sajic, Julien Vaucher, Kim Wiskott, Marc Augsburger, Tony Fracasso, Federica Gilardi, Aurelien Thomas
Summary: Human exposure to arsenic through various sources leads to its accumulation in organs and adipose tissue, contributing to the development of metabolic disorders such as obesity and type 2 diabetes. Acute exposure to low levels of arsenic alters adipocyte functions and decreases insulin sensitivity, exacerbating metabolic dysregulation.
Article
Biochemistry & Molecular Biology
Gaurav Baranwal, Rachel Pilla, Bethany L. Goodlett, Aja K. Coleman, Cristina M. Arenaz, Arul Jayaraman, Joseph M. Rutkowski, Robert C. Alaniz, Brett M. Mitchell
Summary: Recent metabolomics studies have identified a wide array of microbial metabolites and metabolite pathways that are significantly altered in hypertension. Common changes in serum metabolites were found in experimental hypertension models, but not in urine metabolites, indicating the potential association between gut dysbiosis and hypertension needs further exploration.
Article
Peripheral Vascular Disease
Mingyu Zhang, Tammy M. Brady, Jessie P. Buckley, Lawrence J. Appel, Xiumei Hong, Guoying Wang, Liming Liang, Xiaobin Wang, Noel T. Mueller
Summary: This study found that alterations in cord blood metabolome during infancy may be associated with the early life origins of hypertension, particularly related to acylcarnitine and purine metabolism.
Article
Pharmacology & Pharmacy
Caihong Wu, Fang Fang, Yang Yu, Bo Wang, Haicheng Gao, Weiwei Cui
Summary: This study investigated the changes in gut microbiota and metabolomics profile of Wistar rats exposed to cadmium (Cd). The results showed that Cd exposure significantly altered the structure of the gut microbial community and reduced microbiome diversity. Metabolomics analysis revealed marked changes in glycerophospholipid metabolites. These findings suggest that subacute Cd exposure can disrupt the intestinal microecological equilibrium and glycerophospholipid metabolic homeostasis.
Article
Biochemistry & Molecular Biology
Marissa R. Pallotto, Patricia M. Oba, Maria R. C. de Godoy, Kirk L. Pappan, Preston R. Buff, Kelly S. Swanson
Summary: The study utilized metabolomic techniques to analyze the effects of reduced food intake and weight loss in overweight cats, identifying a continual shift in metabolite profile as weight loss progressed. Most lipid metabolites decreased, but ketone bodies and small lipid particles increased with weight loss, while carbohydrate metabolites decreased.
Article
Biochemistry & Molecular Biology
Yannick Audet-Delage, Michele Rouleau, Lyne Villeneuve, Chantal Guillemette
Summary: Nucleotide sugar-dependent glycosyltransferases (UGTs) play critical roles in cellular metabolism, and this study reveals the impact of UGT expression on cellular metabolism. The expression of UGTs leads to modifications in multiple biochemical pathways, affecting glycolysis, pyrimidine pathways, and precursors of UDP-glucuronic acid metabolism. Furthermore, alternative splicing-derived isoforms of UGTs induce unique metabolic perturbations and extensive connectivity with other metabolic processes.
Review
Physiology
Michael H. Lee, Thais C. F. Menezes, Julie A. Reisz, Eloara V. M. Ferreira, Brian B. Graham, Rudolf K. F. Oliveira
Summary: Pulmonary arterial hypertension is an incurable disease characterized by dysregulated metabolism at the cellular level in the pulmonary vasculature and impaired exercise oxygen consumption. The precise interactions between altered pulmonary vascular metabolism and abnormal exercise physiology are relatively unknown.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Pharmacology & Pharmacy
A. Dushani C. U. Ranasinghe, Margaret A. Schwarz
Summary: This article summarizes the emerging epigenetic mechanisms, including DNA methylation, histone modifications, and microRNAs, that contribute to the evolution of pulmonary arterial hypertension (PAH). The interplay between epigenetics and metabolism is explored and new crosstalk targets for investigation in PAH are proposed. The article also highlights the therapeutic opportunities and challenges associated with epigenetics and metabolomics in PAH, including early detection, personalized dietary plans, and advanced drug therapy.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Public, Environmental & Occupational Health
Tao Yu, Hanna Wu, Qingxia Huang, Fen Dong, Xuexin Li, Yushi Zhang, Ruirui Duan, Hongtao Niu, Ting Yang
Summary: This study aimed to determine whether it is possible to predict PM2.5-induced acute exacerbation of COPD (AECOPD) using metabolic markers. Metabolomic analysis identified changes in multiple metabolic pathways induced by PM2.5 exposure, and arginine and glycochenodeoxycholic acid were found to be associated with AECOPD.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Biochemistry & Molecular Biology
Guangtao Qian, Mingyu Wang, Xiaoting Wang, Kai Liu, Ying Li, Yuanyuan Bu, Lixin Li
Summary: Rice, a crucial crop globally, is severely affected by saline-alkali stress, leading to reduced yield and quality. This study investigated the impact of long-term saline-alkali stress on rice by analyzing both the transcriptome and metabolome. The results revealed significant changes in gene expression and metabolites, with 9347 differentially expressed genes (DEGs) and 693 differentially accumulated metabolites (DAMs) observed under high saline-alkali stress (pH > 9.5). The enriched pathways included ABC transporter, amino acid biosynthesis and metabolism, glyoxylate and dicarboxylate metabolism, glutathione metabolism, TCA cycle, and linoleic acid metabolism. These findings highlight the crucial role of metabolites and pathways in rice's response to high saline-alkali stress and provide insights for breeding saline-alkali resistant rice through molecular design.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Mai M. Alzaydi, Vahitha B. Abdul-Salam, Harry J. Whitwell, Giusy Russomanno, Angelos Glynos, Daria Capece, Gyorgy Szabadkai, Martin R. Wilkins, Beata Wojciak-Stothard
Summary: Mitochondrial fission and metabolic switch contribute to vascular pathology in pulmonary arterial hypertension. In this study, the researchers found that the intracellular chloride channel proteins CLIC1 and CLIC4 play a key role in mitochondrial dysfunction and energy metabolism in PAH endothelium.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2023)
Article
Respiratory System
Athenais Boucly, Ly Tu, Christophe Guignabert, Christopher Rhodes, Pascal De Groote, Gregoire Prevot, Emmanuel Bergot, Arnaud Bourdin, Antoine Beurnier, Anne Roche, Mitja Jevnikar, Xavier Jais, David Montani, Martin R. Wilkins, Marc Humbert, Olivier Sitbon, Laurent Savale
Summary: In this study, a three-biomarker panel consisting of beta-NGF, CXCL9, and TRAIL in serum was identified to be independently associated with prognosis in patients with PAH. These biomarkers showed stronger prognostic value for survival compared to conventional non-invasive variables such as NYHA Functional Class, 6-min walk distance, and BNP/NT-proBNP. The results were validated in an independent external validation cohort.
EUROPEAN RESPIRATORY JOURNAL
(2023)
Article
Cardiac & Cardiovascular Systems
Kellen Hirsch, Stephanie Nolley, David D. Ralph, Ying Zheng, William A. Altemeier, Christopher J. Rhodes, Nicholas W. Morrell, Martin R. Wilkins, Peter J. Leary, Samuel G. Rayner
Summary: This study compared the differences in inflammatory and angiogenic biomarker profiles among patients with different etiologies of pulmonary arterial hypertension (PAH) and their association with clinical outcomes. The results showed that patients with connective tissue disease-associated PAH (CTD-PAH) had significantly elevated levels of inflammatory and angiogenic biomarkers. Six biomarkers were associated with mortality in the entire PAH cohort, with IL-6 and NT-proBNP remaining significant after controlling for multiple comparisons.
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2023)
Article
Cell Biology
Md Khadem Ali, Xuefei Tian, Lan Zhao, Katharina Schimmel, Christopher J. Rhodes, Martin R. Wilkins, Mark R. Nicolls, Edda F. Spiekerkoetter
Summary: Bone morphogenic protein receptor 2 (BMPR2) expression and signaling are impaired in pulmonary arterial hypertension (PAH), and the mechanisms behind the decrease in BMPR2 signaling in PAH are poorly understood. This study identified protein tyrosine phosphatase 1B (PTPN1) as a novel regulator of BMPR2 signaling in pulmonary arterial endothelial cells (PAECs). PTPN1 was found to be downregulated in the blood of PAH patients and its downregulation was linked to endothelial dysfunction in PAECs. These findings provide insights into the molecular mechanisms involved in PAH and suggest a potential role for PTPN1 in the disease.
Article
Cardiac & Cardiovascular Systems
Christophe Guignabert, Laurent Savale, Athenais Boucly, Raphael Thuillet, Ly Tu, Mina Ottaviani, Christopher J. Rhodes, Pascal De Groote, Gregoire Prevot, Emmanuel Bergot, Arnaud Bourdin, Luke S. Howard, Elie Fadel, Antoine Beurnier, Anne Roche, Mitja Jevnikar, Xavier Jais, David Montani, Martin R. Wilkins, Olivier Sitbon, Marc Humbert
Summary: Activin A and FSTL3 serum levels are associated with transplant-free survival in PAH. They can serve as prognostic biomarkers for PAH, and their diagnostic values have been confirmed in an independent validation cohort. The activin signaling system in PAH involves phosphorylated Smad2/3 nuclear accumulation and differential immunoreactivities of various proteins in vascular endothelial and smooth muscle layers.
Article
Cardiac & Cardiovascular Systems
Rachel Walters, Eleni Vasilaki, Jurjan Aman, Chien-Nien Chen, Yukyee Wu, Olin D. Liang, Ali Ashek, Olivier Dubois, Lin Zhao, Farah Sabrin, Ines Cebola, Jorge Ferrer, Nicholas W. Morrell, James R. Klinger, Martin R. Wilkins, Lan Zhao, Christopher J. Rhodes
Summary: Pulmonary arterial hypertension (PAH) is a rare disease characterized by remodeling of the pulmonary arteries, increased vascular resistance, and right-sided heart failure. This study found that genetic risk variants in the enhancer region upstream of transcription factor SOX17 are associated with PAH, and these variants impair the binding and expression of SOX17, leading to disturbed endothelial cell function and PAH development. The results also showed that existing drug compounds can reverse the abnormal transcriptomic signature of SOX17 in pulmonary endothelial cells.
Letter
Respiratory System
Fu-Chian Yeh, Chien-Nien Chen, Chong-Yang Xie, Nicoleta Baxan, Lin Zhao, Ali Ashek, Farah Sabrin, Allan Lawrie, Martin Wilkins, Lan Zhao
EUROPEAN RESPIRATORY JOURNAL
(2023)
Article
Pediatrics
Saisujani Rasiah, Peter Jueni, Michael D. Sgro, Kevin E. Thorpe, Jonathon Maguire, Sloane J. Freeman
Summary: This study examined whether a school-based health center model improved academic achievement. The results showed that the model did not have a significant impact on academic achievement, but the wait time was shorter compared to the provincial wait time.
PEDIATRIC RESEARCH
(2023)
Article
Multidisciplinary Sciences
Gary Leggatt, Guo Cheng, Sumit Narain, Luis Briseno-Roa, Jean-Philippe Annereau, Christine Gast, Rodney D. Gilbert, Sarah Ennis
Summary: Autosomal recessive whole gene deletions of NPHP1 result in abnormal cilia structure and function, leading to kidney, retinal, and neurological diseases. This study suggests that SNV mutations have been under-reported in NPHP1-related diseases and may be more common than previously appreciated.
SCIENTIFIC REPORTS
(2023)
Article
Cardiac & Cardiovascular Systems
Eliano Pio Navarese, Antonio Landi, Angelo Oliva, Raffaele Piccolo, Victor Aboyans, Dominick Angiolillo, Dan Atar, Davide Capodanno, Keith A. A. Fox, Sigrun Halvorsen, Stefan James, Peter Juni, Vijay Kunadian, Sergio Leonardi, Roxana Mehran, Gilles Montalescot, Josef Niebauer, Susanna Price, Robert F. Storey, Heinz Voller, Pascal Vranckx, Stephan Windecker, Marco Valgimigli
Summary: Two network meta-analyses were conducted to evaluate all available antithrombotic treatments within or after 12 months following coronary revascularization and/or acute coronary syndrome. Ticagrelor monotherapy showed lower cardiovascular mortality risk within 12 months, and P2Y(12) monotherapy, especially ticagrelor, was associated with lower risk of myocardial infarction beyond 12 months. Aspirin and rivaroxaban 2.5 mg were most effective in reducing stroke risk, with a more acceptable bleeding risk than VKA.
EUROPEAN HEART JOURNAL-CARDIOVASCULAR PHARMACOTHERAPY
(2023)
Article
Critical Care Medicine
Chien-Nien Chen, Nabil Hajji, Fu-Chiang Yeh, Sunniyat Rahman, Souad Ali, John Wharton, Nicoleta Baxan, Lin Zhao, Chong-Yang Xie, Yi-Guan Chen, Maria G. Frid, Prakash Chelladurai, Soni Savai Pullamsetti, Kurt R. Stenmark, Martin R. Wilkins, Lan Zhao
Summary: This study reveals the potential of light control in biological function remodeling and disease diagnosis and treatment, providing important theoretical basis for the development of phototherapy methods in the future.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Article
Genetics & Heredity
Carrie L. Welch, Micheala A. Aldred, Srimmitha Balachandar, Dennis Dooijes, Christina A. Eichstaedt, Stefan Graef, Arjan C. Houweling, Rajiv D. Machado, Divya Pandya, Matina Prapa, Memoona Shaukat, Laura Southgate, Jair Tenorio-Castano, Wendy K. Chung
Summary: The Chinese Society of Medical Genetics has proposed a scoring system for evaluating the strength of evidence for genetic causality in pulmonary arterial hypertension (PAH). The results indicate that 12 genes have definitive evidence for a link to PAH, 3 genes have moderate evidence, 6 genes have limited evidence, and 1 gene has no known relationship to PAH. The recommendation is to include genes with definitive evidence in genetic testing and to interpret variants in genes with moderate or limited evidence with caution, while excluding genes with no evidence or controversy.
GENETICS IN MEDICINE
(2023)
Article
Immunology
Aaron Campigotto, Allison Chris, Julia Orkin, Lynette Lau, Christian Marshall, Ari Bitnun, Sarah A. Buchan, Liane MacDonald, Nisha Thampi, Janine McCready, Peter Juni, Rulan S. Parekh, Michelle Science
Summary: This study demonstrates the use of whole genome sequencing to investigate SARS-CoV-2 transmission within schools. The results show genetically distinct viruses within different clinical cohorts in the same school. This approach provides valuable insights into the timing of transmission, the effectiveness of mitigation measures, and can help avoid unnecessary school closures.
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2023)