Article
Hematology
Hannah Kinoshita, Mamatha Mandava, Mariah Jensen-Wachspress, Haili Lang, Elisabeth Joy, Jay Tanna, Chase D. McCann, Samuel O'Brien, Sianna Burnett, Abeer Shibli, Fahmida Hoq, Monica Bhatia, Patrick J. Hanley, Blachy Davila Saldana, Kris M. Mahadeo, Catherine M. Bollard, Michael D. Keller, Allistair Abraham
Summary: Hematopoietic stem cell transplantation (HSCT) is increasingly used to treat sickle cell disease (SCD). However, this treatment increases the risk of viral infection. This study explores the use of virus-specific T-cell (VST) therapies after HSCT to prevent or treat viral infections in SCD patients.
Article
Biophysics
Alice Pievani, Valentina Granata, Giacomo Desantis, Laura Antolini, Sara Ornaghi, Antonio Galleu, Andrea Biondi, Bernhard Gentner, Francesco Dazzi, Marta Serafini
Summary: Monocytes are effective at improving engraftment in HSPC transplantation, particularly in cases of limited cell doses such as CB transplantation and HSC gene therapy. Co-culturing monocytes increases survival and stemness of CB-CD34(+) cells. Soluble factors and direct-cell contact interactions play critical roles in the HSC-monocyte crosstalk.
BONE MARROW TRANSPLANTATION
(2022)
Article
Medicine, General & Internal
Alice Bertaina, Paul C. Grimm, Kenneth Weinberg, Robertson Parkman, Karen M. Kristovich, Giulia Barbarito, Elizabeth Lippner, Girija Dhamdhere, Vasavi Ramachandran, Jordan M. Spatz, Sahar Fathallah-Shaykh, T. Prescott Atkinson, Amira Al-Uzri, Geraldine Aubert, Kim van der Elst, Sean G. Green, Rajni Agarwal, Priscila F. Slepicka, Ami J. Shah, Maria G. Roncarolo, Amy Gallo, Waldo Concepcion, David B. Lewis
Summary: This study aimed to find a new approach to eliminate immune rejection and the need for post-transplantation immunosuppression. By performing bone marrow and kidney transplantation on three patients with a specific genetic condition, the researchers successfully achieved the goal of normal renal function without the need for immunosuppression.
NEW ENGLAND JOURNAL OF MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Daniel E. Maidana, Lucia Gonzalez-Buendia, Joan W. Miller, Demetrios G. Vavvas
Summary: Recent evidence suggests that modulating the RIPK3 pathway can improve the survival of transplanted cells and aid in the repair of degenerated retinas. Simultaneous inhibition of RIPK3 in both the donor and host can maximize the survival of transplanted cells. Additionally, targeting RIPK3 in the immune system can protect both donor and host retinal cells. These findings highlight the potential of immunomodulatory and neuroprotective strategies targeting RIPK3 in the regenerative therapy of photoreceptor transplantation.
Article
Endocrinology & Metabolism
Vicente F. C. Andrade, Debora Besen, Domingos C. Chula, Victoria Z. C. Borba, David Dempster, Carolina Aguiar Moreira
Summary: This study found that poor glycemic control is associated with hyperplasia and hypertrophy of BMAs and lower BV/TV. The new BMA parameter Ad.N/BS is correlated with HbA1c and negatively with O.Th. The use of insulin seems to stimulate the expansion of BMA while metformin has the opposite effect.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2021)
Article
Endocrinology & Metabolism
Vicente F. C. Andrade, Debora Besen, Domingos C. Chula, Victoria Z. C. Borba, David Dempster, Carolina Aguiar Moreira
Summary: This study found that poor glycemic control is associated with hyperplasia and hypertrophy of BMAs and with lower BV/TV. A new BMA parameter, Ad.N/BS, is positively correlated with HbA1c and negatively with O.Th. The use of insulin seems to stimulate the expansion of BMA while that of metformin has the opposite effect.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2021)
Article
Biochemistry & Molecular Biology
Zahra Eydian, Alaleh Mohammad Ghasemi, Samira Ansari, Ali Naghi Kamali, Maryam Khosravi, Sima Momtaz, Sanaz Riki, Laleh Rafighdoost, Reza Entezari Heravi
Summary: This study demonstrates the differentiation of mesenchymal stem cells (MSCs) from human adipose tissue and bone marrow into functional beta-islet cells. It suggests that adipose stem cells may be a better choice for the treatment of diabetes mellitus due to their higher potency.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Microbiology
Shiela McCollam, James S. Lewis, Joseph Bubalo, Amber Diaz
Summary: This study aimed to evaluate the efficacy and safety of 300-mg once-monthly intravenous (IV) pentamidine prophylaxis in 702 adult allogeneic hematopoietic stem cell transplant (HSCT) patients. The results showed that IV pentamidine administration effectively prevented Pneumocystis jirovecii pneumonia (PJP), but breakthrough Nocardia and Toxoplasma infections were observed in a certain percentage of patients.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Cell & Tissue Engineering
Liem Thanh Nguyen, Duc M. Hoang, Kien T. Nguyen, Duc M. Bui, Hieu T. Nguyen, Hong T. A. Le, Van T. Hoang, Hue T. H. Bui, Phuong T. M. Dam, Xuan T. A. Hoang, Anh T. L. Ngo, Hang M. Le, Nhi Y. Phung, Duc M. Vu, Trung T. Duong, Tu D. Nguyen, Lien T. Ha, Hoa T. P. Bui, Hoa K. Nguyen, Michael Heke, Anh Bui
Summary: The study suggests that autologous administration of bone marrow-derived mesenchymal stem/stromal cells (BM-MSCs) is safe in patients with type 2 diabetes mellitus (T2DM), with treatment efficacy dependent on the quality of the stem cell population and administration routes. Short-term therapeutic effects were observed in patients with shorter T2DM duration and lower body weight.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Clinical Neurology
Antonio Uccelli, Alice Laroni, Rehiana Ali, Mario Alberto Battaglia, Morten Blinkenberg, Lou Brundin, Michel Clanet, Oscar Fernandez, James Marriot, Paolo Muraro, Seyed Massood Nabavi, Roberto S. Oliveri, Ernst Radue, Cristina Ramo Tello, Irene Schiavetti, Johann Sellner, Per Soelberg Sorensen, Maria Pia Sormani, Jens Thomas Wuerfel, Mark S. Freedman
Summary: The MESEMS study aimed to evaluate the safety and activity of autologous bone marrow-derived MSCs in patients with active multiple sclerosis. The results showed that MSC treatment did not affect GELs and does not support its use in treating multiple sclerosis.
Review
Cell & Tissue Engineering
Gian Paolo Fadini, Mattia Albiero
Summary: During antenatal development, HSPCs arise and migrate to the fetal liver. In adulthood, HSPCs retain migratory activity and circulate to peripheral tissues. Diabetes mellitus jeopardizes HSPC traffic, reducing circulating HSPC levels and increasing the risk for multi-organ damage. The impaired traffic of HSPCs and enhanced generation of pro-inflammatory cells contribute to tissue damage and inflammation.
Article
Multidisciplinary Sciences
Laura Breda, Tyler E. Papp, Michael P. Triebwasser, Amir Yadegari, Megan T. Fedorky, Naoto Tanaka, Osheiza Abdulmalik, Giulia Pavani, Yongping Wang, Stephan A. Grupp, Stella T. Chou, Houping Ni, Barbara L. Mui, Ying K. Tam, Drew Weissman, Stefano Rivella, Hamideh Parhiz
Summary: This study developed a nanoparticle system that can target hematopoietic stem cells in vivo and achieved correction of diseased cells through mRNA delivery. Furthermore, this system enables non-genotoxic conditioning for stem cells, providing a new approach for HSCT.
Article
Medicine, General & Internal
Jeremy Epah, Gabriele Spohn, Kathrin Preiss, Markus M. Mueller, Johanna Doerr, Rainer Bauer, Shabnam Daqiq-Mirdad, Joachim Schwaeble, Stefanie N. Bernas, Alexander H. Schmidt, Erhard Seifried, Richard Schaefer
Summary: This study found that the collection efficiency of bone marrow (BM) samples varies greatly worldwide. The implementation of a small volume aspirate policy improved the total nucleated cell yields in BM products. The impact of the BM collection technique on the quality of hematopoietic progenitor cell-manufacture was assessed.
Article
Medicine, Research & Experimental
Simona Pagliuca, Carmelo Gurnari, Sanghee Hong, Ran Zhao, Sunisa Kongkiatkamon, Laila Terkawi, Misam Zawit, Yihong Guan, Hassan Awada, Ashwin Kishtagari, Cassandra M. Kerr, Thomas LaFramboise, Bhumika J. Patel, Babal K. Jha, Hetty E. Carraway, Valeria Visconte, Navneet S. Majhail, Betty K. Hamilton, Jaroslaw P. Maciejewski
Summary: TCR repertoire diversification is crucial for successful immune reconstitution after allo-HCT. Analyses of deep TCR V beta sequencing revealed that rarefied and hyperexpanded clonotypic patterns are hallmarks of T cell reconstitution, influenced by donor and pretransplant TCR diversity. The expansion of pathogen- and tumor-associated clonotypes was predominant in the late post-allo-HCT phase, while autoreactive clones were more expanded in cases of graft-versus-host disease.
Article
Biology
Zheting Liao, Yu Jin, Yuhao Chu, Hansen Wu, Xiaoyu Li, Zhonghao Deng, Shuhao Feng, Nachun Chen, Ziheng Luo, Xiaoyong Zheng, Liangxiao Bao, Yongqing Xu, Hongbo Tan, Liang Zhao
Summary: A study using single-cell RNA sequencing explored the transcriptomic landscape of alcohol-induced osteonecrosis of the femoral head (ONFH). The study revealed the relevance of stromal and endothelial cells to the pathogenesis of the disease and provided resources for developing cell type-specific therapeutic strategies.
COMMUNICATIONS BIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Chelsea L. Organ, Zhen Li, Thomas E. Sharp, David J. Polhemus, Nilaksh Gupta, Traci T. Goodchild, W. H. Wilson Tang, Stanley L. Hazen, David J. Lefer
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2020)
Review
Physiology
Thomas E. Sharp, David J. Lefer
Summary: Heart failure is a global pandemic with challenges in treatment, particularly in patient tolerance to neurohormonal pharmacotherapies. Renal denervation as a novel therapeutic strategy provides a potential possibility to attenuate the progression of HF.
ANNUAL REVIEW OF PHYSIOLOGY, VOL 83
(2021)
Article
Cardiac & Cardiovascular Systems
Huijing Xia, Zhen Li, Thomas E. Sharp, David J. Polhemus, Jean Carnal, Karl H. Moles, Ya-Xiong Tao, John Elrod, Josef Pfeilschifter, Karl-Friedrich Beck, David J. Lefer
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2020)
Editorial Material
Cardiac & Cardiovascular Systems
Zhen Li, David J. Lefer
CIRCULATION RESEARCH
(2021)
Review
Cardiac & Cardiovascular Systems
Rajesh Kumari, Prabhat Ranjan, Zainab Gbongbo Suleiman, Sumanta Kumar Goswami, Jing Li, Ram Prasad, Suresh Kumar Verma
Summary: m(6)A methylation is a highly studied RNA modification that plays a crucial role in cellular functions during development, differentiation, and disease. It is found in different RNA species and is regulated by various enzymes and proteins. In cardiovascular diseases, m(6)A methylation is associated with cardiac hypertrophy, myocardial infarction, and atherosclerosis.
CARDIOVASCULAR RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Kyle B. LaPenna, David J. Polhemus, Jake E. Doiron, Hunter A. Hidalgo, Zhen Li, David J. Lefer
Summary: Hydrogen sulfide (H2S) is an endogenous gaseous signaling molecule that plays a critical role in cardiac and vascular biology by regulating vascular tone, oxidant defenses, and exerting cytoprotective effects. Recent studies have shown that H2S modulates various components of metabolic syndrome and exhibits cardioprotective signaling in heart failure.
Article
Pharmacology & Pharmacy
Sumanta Kumar Goswami, Prabhat Ranjan, Roshan Kumar Dutta, Suresh Kumar Verma
Summary: Cardiovascular disease is the leading cause of morbidity and mortality worldwide, with inflammation playing a key role in its development. Immune cells and cytokines are crucial in regulating cardiac inflammation, with IL-10 playing a significant role in reducing cardiovascular inflammation and protecting the cardiovascular system.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Cell Biology
Praveen K. Dubey, Mallikarjun Patil, Sarojini Singh, Shubham Dubey, Paras Ahuja, Suresh Kumar Verma, Prasanna Krishnamurthy
Summary: Endotoxemia-induced m6A methylation in the myocardium is associated with increased expression of inflammatory cytokine genes and reduced cardiac function, suggesting a potential role of m6A methylation in regulating myocardial inflammation and dysfunction during endotoxemia.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2022)
Editorial Material
Cardiac & Cardiovascular Systems
Jake E. Doiron, David J. Lefer
CIRCULATION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Eric Goetzman, Zhenwei Gong, Dhivyaa Rajasundaram, Ishan Muzumdar, Traci Goodchild, David Lefer, Radhika Muzumdar
Summary: Acute myocardial infarction is a leading cause of death worldwide. This study investigated the metabolic changes in serum during ischemia and reperfusion injury in a swine model, aiming to identify metabolomic biomarkers for myocardial injury at different phases. The results showed distinct profiles of serum metabolites during ischemia and early and late reperfusion, with impaired mitochondrial function and amino acid modifications during ischemia, and changes in branched-chain amino acid metabolites and bile acid pathway derivatives during reperfusion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Neurosciences
Prabhat Ranjan, Karen Colin, Roshan Kumar Dutta, Suresh Kumar Verma
Summary: The article introduces the concept of exosomes and their applications in disease treatment. Current technological advances provide better ways for monitoring exosomes, but further research is needed to ensure effective clinical applications.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Meeting Abstract
Cardiac & Cardiovascular Systems
Maria Cimini, Venkata Garikipati, Andrea Elia, Chunlin Wang, May Truongcao, Anna Maria Lucchese, Grace Huang, Vandana Mallaredy, Carolina Gonzalez, Cindy Benedict, Raj Kishore
CIRCULATION RESEARCH
(2021)
Meeting Abstract
Cardiac & Cardiovascular Systems
Ajit Magadum, Vandana Mallaredy, Grace Grace, Chunlin Wang, Rajika Roy, Darukeshwara Joladarashi, Charan Thej Gurrala, Zhongjian Cheng, Maria Cimini, May Truongcao, Anna Maria Lucchese, Carolina Gonzalez, Cindy Benedict, Raj Kishore
CIRCULATION RESEARCH
(2021)
Letter
Cardiac & Cardiovascular Systems
Thomas E. Sharp, David J. Lefer, Traci T. Goodchild
JACC-BASIC TO TRANSLATIONAL SCIENCE
(2021)
Article
Cardiac & Cardiovascular Systems
Thomas E. Sharp, Amy L. Scarborough, Zhen Li, David J. Polhemus, Hunter A. Hidalgo, Jeffery D. Schumacher, Timothy R. Matsuura, J. Stephen Jenkins, Daniel P. Kelly, Traci T. Goodchild, David J. Lefer
Summary: This study developed a novel large animal model of HFpEF that replicates the multiple comorbidities associated with human HFpEF, providing a valuable tool for investigating the pathogenesis of the disease and testing new therapeutic strategies.
JACC-BASIC TO TRANSLATIONAL SCIENCE
(2021)