Article
Environmental Sciences
Liben Lin, Qiang Xu, Quanxu Chen, Haiqiong Chen, Yingfen Ying, Zengqiang Li, Song Zhang, Feifei Ma, Yige Yu, Ren-Shan Ge
Summary: Triadimefon inhibits the development of fetal Leydig cells in male fetuses by suppressing their differentiation, as well as inducing abnormal aggregation of these cells.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Environmental Sciences
Renata M. P. Freitas, Barbara S. Linhares, Jerusa M. Oliveira, Joao Paulo Leite, Sergio Luis P. da Matta, Reggiani Goncalves, Mariella B. Freitas
Summary: The fungicide TEB induces oxidative stress in Neotropical fruit-eating bats, resulting in liver damage and hormonal imbalance. Co-administration of the F. carica plant extract attenuates some oxidative stress responses and liver damage in exposed bats.
Article
Reproductive Biology
Xiaodan Li, Xiaolei Yao, Haiqiang Xie, Mingtian Deng, Xiaoxiao Gao, Kaiping Deng, Yongjin Bao, Qi Wang, Feng Wang
Summary: This study revealed that SPATA6 is highly expressed in the testis of Hu sheep and localized in LCs and spermatogenic cells. SPATA6 plays a key role in regulating the proliferation, apoptosis, and testosterone biosynthesis of LCs, contributing to sheep spermatogenesis.
Article
Andrology
Lili Tian, Xueyun Li, Yiyan Wang, Quanxu Chen, Xiaoheng Li, Ren-Shan Ge, Xingwang Li
Summary: Oncostatin M stimulates the proliferation of immature Leydig cells while inhibiting the function of immature and adult Leydig cells, affecting cell cycle and testosterone synthesis.
Article
Environmental Sciences
Won-Young Lee, Ran Lee, Hyun-Jung Park
Summary: Tebuconazole (TEB), a triazole fungicide, was found to decrease cell viability and proliferation and induce apoptotic cell death and endoplasmic reticulum (ER) stress in bovine mammary gland epithelial cells (MAC-T cells). TEB activated pro-apoptotic proteins and upregulated ER stress markers, leading to mitochondria-mediated apoptotic cell death. Additionally, TEB caused a reduction in the expression of genes related to milk protein synthesis in MAC-T cells, suggesting potential negative effects on milk production in dairy cows.
Article
Reproductive Biology
Hong Chen, Kexing Chen, Fange Zhao, Yihan Guo, Yue Liang, Zhengrong Wang, Tengfei Liu, Shulin Chen
Summary: This study investigated the steroidogenic activity and autophagy levels of Leydig cells (LCs) in dairy goats at different stages of development. The results showed that the steroidogenic activity and ultrastructural characteristics of LCs changed with age, and autophagy activity was enhanced in the testes at pubertal and adult stages. In addition, the study found that autophagy affects testosterone production in LCs.
Article
Biochemistry & Molecular Biology
Sha Han, Jiaqiang Luo, Shuai Xu, Liangyu Zhao, Chencheng Yao, Junwei Xu, Ruhui Tian, Erlei Zhi, Yuhua Huang, Shujie Xia, Zheng Li, Peng Li
Summary: Aging negatively affects human testicular function, leading to impaired steroidogenesis and decreased testosterone levels. Low-intensity pulsed ultrasound (LIPUS) has been shown to increase testosterone levels in animal models and is a non-invasive treatment option for male erectile dysfunction. This study investigates the effect of LIPUS on human senescent Leydig cells (LCs) and finds that stimulation with LIPUS improves the senescence phenotype and enhances LCs' secretory function, increasing the expression of key pathways in testosterone synthesis. These findings suggest that LIPUS may be a novel treatment for human senescent LCs with decreased testosterone secretion levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Xuan Sun, Yifei Wang, Qingyang Mai, Yixuan Fan, Qunhao Huang, Yingli Du, Hai Huang, El-Samahy Ma, Feng Wang, Guomin Zhang
Summary: The study reveals that Neuromedin S (NMS) and its receptors are mainly expressed in Leydig cells (LCs) of goat testes, with the highest expression detected at age three months. NMS addition significantly enhances testosterone secretion and cell proliferation in vitro cultured goat LCs by modulating mitochondrial morphology, function, and autophagy. These findings provide a novel view of the regulatory mechanisms involved in male sexual maturation.
Article
Food Science & Technology
Huitao Li, Zina Wen, Chaobo Ni, Xianwu Chen, Yong Cheng, Yuxin Liu, Xiaoheng Li, Qiqi Zhu, Ren-Shan Ge
Summary: Short-term exposure to PFDoA in adulthood delayed Leydig cell regeneration by preventing Leydig cells from stem cells through multiple mechanisms.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jinlong Zhang, Rongrong Ye, Jason William Grunberger, Jiaqi Jin, Qianru Zhang, Raziye Mohammadpour, Nitish Khurana, Xianyu Xu, Hamidreza Ghandehari, Fenglei Chen
Summary: This study found that low doses of SNPs can enhance testosterone secretion by activating autophagy in PLCs. SNPs promote autophagy process by altering the expression levels of autophagy-related proteins. Furthermore, SNPs also increase the expression of steroidogenic acute regulatory protein, further enhancing testosterone production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Endocrinology & Metabolism
Indrashis Bhattacharya, Souvik Dey
Summary: Leydig cells (Lc) in the testis play a critical role in male fertility by producing testosterone in response to luteinizing hormone (LH). Recent studies have identified the diverse origins of these cells and their differential transcriptomic and steroidogenic outputs. Fetal Lc (FLc) originate from coelomic epithelium and perivascular cells, and gradually give way to adult Lc (ALc), which are considered to be derived from FLc and perivascular cells. This article summarizes the current understanding of Lc development, their cellular origins, and their functional significance in rodent and primate testes.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ylenia Marino, Alessia Arangia, Ramona D'Amico, Marika Cordaro, Rosalba Siracusa, Daniela Impellizzeri, Enrico Gugliandolo, Roberta Fusco, Salvatore Cuzzocrea, Rosanna Di Paola
Summary: This study investigated the toxic effects of TEB on autoimmune myocarditis (AM). The results showed that TEB exposure aggravated cardiac damage induced by AM, increased inflammation, and increased fibrosis and collagen deposition. It also altered the balance of oxidative stress and antioxidant enzymes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Xueyun Li, Fangyan Meng, Lei Ye, Xinyi Qiao, Jingjing Li, Lili Tian, Ming Su, Liben Lin, Ren-shan Ge, Yiyan Wang
Summary: This study found that in utero exposure to TMBPA can lead to morphological and functional alterations of fetal Leydig cells in male offspring. High dose exposure to TMBPA promotes fetal Leydig cell proliferation via the ESR1-CDK2/4-AKT pathway and inhibits cell function by reducing steroidogenic enzyme activity.
ENVIRONMENTAL TOXICOLOGY
(2022)
Article
Cell Biology
Yabing Chen, Jing Wang, Dihui Xu, Zou Xiang, Jie Ding, Xiaoyu Yang, Dongmei Li, Xiaodong Han
Summary: The study reveals a negative association between m(6)A modification and autophagy in Leydig cells during testosterone synthesis. The regulation of METTL14, ALKBH5, and m(6)A influences AMP-activated protein kinase activity and subsequent autophagy in Leydig cells. Human chorionic gonadotropin treatment modulates m(6)A modification by upregulating ALKBH5 and decreasing METTL14, affecting the synthesis of testosterone.
Article
Environmental Sciences
Xinrui Hao, Xiaoju Guan, Xingxing Zhao, Minpeng Ji, Xin Wen, Panpan Chen, Fenfen Chen, Jianying Yang, Qingquan Lian, Leping Ye, Haolin Chen
Summary: Studies have shown that phthalates, such as MEHP, can significantly inhibit the proliferation and differentiation of stem Leydig cells (SLCs). It also affects the differentiation of SLCs into Leydig cells and adipocytes.
