4.7 Article

Survey of dissimilatory nitrate reduction to ammonium microbial community at national wetland of Shanghai, China

期刊

CHEMOSPHERE
卷 250, 期 -, 页码 -

出版社

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2020.126195

关键词

Dissimilatory nitrate reduction to ammonium; Functional gene nrfA; Microorganism diversity; Wetland sediments; Yangtze river estuary

资金

  1. Open Research Fund of State Key Laboratory of Estuarine and Coastal Research [SKLEC-KF201909]
  2. National Natural Science Foundation of China [21777086]
  3. Taishan Scholar Youth Expert Program of Shandong Province [tsqn201909005]
  4. Key Research & Developmental Program of Shandong Province [2019JZZY020308]
  5. Natural Science Foundation for Distinguished Young Scholars of Shandong Province [JQ201809]
  6. Young Scholars Program of Shandong University [2016WLJH16, 2020QNQT012]
  7. Shandong Provincial Water Conservancy Research and Technology Promotion Project [SDSLKY201802]
  8. Open Project Program of State Key Laboratory of Petroleum Pollution Control [PPC2018007]
  9. CNPC Research Institute of Safety and Environmental Technology
  10. China Association of Marine Affairs (CAMA)
  11. Association of Ocean of China (AOC) [CAMAJJ201808]

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Dissimilatory nitrate reduction to ammonia (DNRA) process is an important nitrate reduction pathway in the environment. Numerous studies focused on the DNRA, especially in various natural habitats. However, little is known about the envrionmental parameters driving the DNRA process in anthropogenic ecosystem. Human activities put forward significant influence on nitrogen cycle and bacterial communities of sediment. This study aimed to assess the DNRA potential rates, nrfA gene abundance, DNRA bacterial community's diversity and influencing factors in a national wetland park near the Yangtze River estuary, Shanghai. The results of N-15 isotope tracer experiments showed that DNRA potential rates from 0.13 to 0.44 mu mol N/kg/h and contribution of nitrate reduction varied from 1.56% to 7.47%. The quantitative real-time PCR results showed that DNRA functional gene nrfA abundances ranged from 9.87E+10 to 1.98E+11 copies/g dry weight. The results of nrfA gene pyrosequencing analysis showed that Lacunisphaera (10.4-13.4%), Sorangium (7.1-10.7%), Aeromonas (4.2-6.8%), Corallococcus (1.8-6.9%), and Geobacter (3.3-6.6%) showed higher relative abundances in their genus levels. Combined with environmental parameters of sediments, redundancy analysis indicated that the nrfA functional gene was positively correlated with moisture content, the concentration of NO (2) over bar -N and NO(3) over barN; the DNRA rates was positively correlated with sediment organic carbon (SOC), C/NO(3) over bar ratio and salinity (ranked by explains %). This study is the first simultaneous determination of nitrate reduction pathways including denitrification, anammox and DNRA rates to assess the role of DNRA in a national wetland park and revealed the community abundance, diversity of DNRA bacteria and its relationship with environmental factors. (C) 2020 Elsevier Ltd. All rights reserved.

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