期刊
CELL CYCLE
卷 19, 期 14, 页码 1777-1785出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15384101.2020.1779470
关键词
Meiosis; fission yeastSchizosaccharomyces pombe; silac; short open reading frames
类别
资金
- Slovak Grant Agency VEGA [1/0450/18, 2/0039/19, 2/0034/19, 2/0026/18]
- Slovak Research and Development Agency [APVV-17-0130, APVV-18-0219, APVV-16-0120]
- Biotechnology and Biological Sciences Research Council [BB/M021483/1]
- Austrian Science Fund (FWF) [P30516]
- Agentura na Podporu Vyskumu a Vyvoja [APVV-17-0130, APVV-18-0219, APVV-16-0120]
- Austrian Science Fund [P30516]
- Vedecka Grantova Agentura MSVVaS SR a SAV [1/0450/18, 2/0039/19, 2/0026/18]
- Austrian Science Fund (FWF) [P30516] Funding Source: Austrian Science Fund (FWF)
- BBSRC [BB/M021483/1] Funding Source: UKRI
Meiosis is the process by which haploid gametes are produced from diploid precursor cells. We used stable isotope labeling by amino acids in cell culture (SILAC) to characterize the meiotic proteome in the fission yeastSchizosaccharomyces pombe. We compared relative levels of proteins extracted from cells harvested around meiosis I with those of meiosis II, and proteins from premeiotic S phase with the interval between meiotic divisions, when S phase is absent. Our proteome datasets revealed peptides corresponding to short open reading frames (sORFs) that have been previously identified by ribosome profiling as new translated regions. We verified expression of selected sORFs by Western blotting and analyzed the phenotype of deletion mutants. Our data provide a resource for studying meiosis that may help understand differences between meiosis I and meiosis II and how S phase is suppressed between the two meiotic divisions.
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