Article
Immunology
Jin Li, Qiu Zhong, Mei-Yun Shang, Min Li, Yuan-Su Jiang, Jia-Jun Zou, Shan-Shan Ma, Qing Huang, Wei-Ping Lu
Summary: In this study, a simple and visual device combined with lateral flow strip-based recombinase polymerase amplification (LF-RPA) was developed for the identification of Burkholderia pseudomallei. The LF-RPA assay showed good performance in amplifying target DNA at a temperature range of 30-45 degrees C and completed the entire reaction in a short time. It had a low limit of detection for B. pseudomallei genomic DNA, comparable to real-time PCR. The newly developed LF-RPA system was also successfully used to retrospectively confirm clinical B. pseudomallei isolates.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Medicine, General & Internal
Tingting Jiang, Yacui Wang, Weiwei Jiao, Yiqin Song, Qing Zhao, Tianyi Wang, Jing Bi, Adong Shen
Summary: In this study, a real-time recombinase polymerase amplification (RPA) assay was developed for rapid and accurate detection of Mycoplasma pneumoniae. The assay showed high sensitivity, fast amplification, ease of use, and no cross-reaction with other respiratory pathogens. It has the potential to serve as a promising test for early diagnosis of M. pneumoniae infection.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Immunology
Ruiwen Li, Jinfeng Wang, Xiaoxia Sun, Libing Liu, Jianchang Wang, Wanzhe Yuan
Summary: This study developed and validated isothermal recombinase polymerase amplification (RPA) assays for quick and direct detection of Mycoplasma bovis in bovine milk. The RPA assays showed high specificity and efficiency in detecting M. bovis in milk samples, making them promising tools for rapid response to bovine mastitis caused by M. bovis infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Yuelin Liu, Jialin Xiang, Yaxin Gao, Jinfeng Wang, Libing Liu, Ruiwen Li, Jianchang Wang
Summary: In this study, real-time RPA and LFS RPA assays were developed for the rapid detection of Cryptosporidium spp. The assays showed high specificity and sensitivity, and were able to detect multiple Cryptosporidium species.
Article
Biotechnology & Applied Microbiology
Xiaohan Yang, Panpan Zhao, Yu Dong, Shiqi Chen, Hui Shen, Ge Jiang, Hai Zhu, Jingquan Dong, Song Gao
Summary: A new rapid detection method for V. vulnificus was developed using RPA technology and LFS strip, with good specificity and sensitivity, suitable for on-site detection.
Article
Microbiology
Silvia Goncalves Mesquita, Elena Birgitta Lugli, Giovanni Matera, Cristina Toscano Fonseca, Roberta Lima Caldeira, Bonnie Webster
Summary: Recombinase Polymerase Amplification (RPA) is a simple, rapid, sensitive, and specific method for detecting Schistosoma mansoni. In this study, RPA demonstrated good specificity and sensitivity, allowing for stable detection of S. mansoni DNA in human urine and stool samples.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Yuelin Liu, Libing Liu, Jinfeng Wang, Xiaoxia Sun, Yaxin Gao, Wanzhe Yuan, Jianchang Wang, Ruiwen Li
Summary: In this study, two RT-RPA assays (real-time RT-RPA and LFS RT-RPA) were successfully developed for the rapid detection of bovine rotavirus A (BRVA) in cattle fecal samples. The assays showed high specificity, sensitivity, and diagnostic accuracy. The developed RT-RPA assays have great potential for use in under-equipped diagnostic laboratories and point-of-need diagnosis at quarantine stations and farms, which is important for controlling BRVA-associated diarrhea in cattle herds.
BMC VETERINARY RESEARCH
(2022)
Article
Immunology
Duobao Ge, Fang Wang, Yanyan Hu, Bendi Wang, Xuzhu Gao, Zhenxing Chen
Summary: This study combined isothermal recombinase polymerase amplification (RPA) with nanoparticle-based lateral flow strips (LFS) to establish a method for the rapid detection of Porphyromonas gingivalis. The method showed high specificity and sensitivity, with a completion time of 30 minutes and visual detection of the amplification products. It simplifies the tedious process of detecting P. gingivalis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Analytical
Ting Zheng, Xianming Li, Ya-Ni Xie, Bin Yang, Peng Wu
Summary: This study proposes a dual-gene RPA-LFA method to selectively detect E. coli O157:H7 by recognizing target amplicons and eliminating false positives. The assay shows high sensitivity and specificity for the detection of this pathogen.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Yong Sun, Panzhu Qin, Jun He, Weiwei Li, Yonglin Shi, Jianguo Xu, Qian Wu, Qingqing Chen, Weidong Li, Xinxin Wang, Guodong Liu, Wei Chen
Summary: This work introduces a new detection technology that can simultaneously test for SARS-CoV-2 and influenza viruses, with advantages such as speed, high sensitivity, no cross-reactivity, and false positives. Its clinical application demonstrates high accuracy, and this portable device is expected to meet the global healthcare system's need for online monitoring of serious infectious diseases.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Microbiology
Cassandra Suther, Sloane Stoufer, Yanjiao Zhou, Matthew D. Moore
Summary: Foodborne and enteric viruses impose a significant burden on public health and economy globally. Real time PCR, although considered a gold standard, has limitations in terms of portability, sample processing, and time to result. Isothermal amplification methods have shown promise as portable, sensitive, and rapid alternatives to PCR for detecting these viruses.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Chemistry, Physical
Madalin Alexandru Cobzariu, Maria Jesus Lobo-Castanon, Rebeca Miranda-Castro
Summary: The detection of genetic material is crucial in clinical practice, food quality control, and environmental analysis. Nucleic acid tests involve amplification to detect the low amount of target sequences, with enzyme-assisted isothermal amplification strategies such as HDA, LAMP, and RPA being easier to adapt to electrochemical platforms for point-of-need applications compared to PCR. This review discusses the current state and challenges of coupling these amplification strategies to conductive platforms for electrochemical transduction in real-world point-of-need tests.
CURRENT OPINION IN ELECTROCHEMISTRY
(2023)
Article
Microbiology
Teck-Phui Chua, Jennifer Danielewski, Kaveesha Bodiyabadu, Catriona S. Bradshaw, Dorothy A. Machalek, Suzanne M. Garland, Erica L. Plummer, Lenka A. Vodstrcil, Gerald L. Murray
Summary: This study investigates the association between single nucleotide polymorphisms (SNPs) in the 16S rRNA gene of Mycoplasma genitalium and change in organism load. Though doxycycline is not highly effective at eradicating M. genitalium infections, it can reduce organism load. The study concludes that the low efficacy of doxycycline against M. genitalium is not due to variation in the 16S rRNA gene.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Review
Biotechnology & Applied Microbiology
Alangar I. Bhat, Rashid Aman, Magdy Mahfouz
Summary: Plant diseases caused by viruses have a significant impact on crop production and quality, resulting in substantial losses. The options for managing viruses are limited due to their nature as systemic obligate parasites. In this regard, sensitive, robust, and affordable diagnostic assays are needed to detect the presence of viruses in plant materials and prevent their spread. Traditional methods based on biological and physical properties have limitations in terms of sensitivity and time consumption. On the other hand, assays based on viral proteins and nucleic acids offer more specificity, sensitivity, and rapidity. However, they often require sophisticated equipment and technical skills in laboratory settings. Isothermal-based assays, such as LAMP and RPA, provide a simple and reliable alternative that does not require specialized equipment or skills. These assays can be performed using lateral flow devices, making them suitable for onsite detection or testing in the field. Isothermal amplification assays can also be combined with CRISPR/Cas technology to overcome non-specific amplification and cross-contamination issues. The collateral activity associated with some CRISPR/Cas systems has been successfully utilized for the visual detection of plant viruses.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Microbiology
Feina Li, Jing Xiao, Haiming Yang, Yao Yao, Jieqiong Li, Huiwen Zheng, Qian Guo, Xiaotong Wang, Yuying Chen, Yajie Guo, Yonghong Wang, Chen Shen
Summary: In this study, a rapid and efficient method utilizing RPA-CRISPR/Cas12a technology was proposed for the diagnosis of Mycoplasma pneumoniae. This method eliminates the need for expensive instruments and specialized personnel. The entire process, including sample extraction, RPA reaction, CRISPR/Cas12a detection, and visual observation, can be completed within 1 hour with high sensitivity and specificity.
FRONTIERS IN MICROBIOLOGY
(2022)
