Studies on laccase mediated conversion of lignin from ginseng residues for the production of sugars

The purpose of this study was to determine the production of sugars from ginseng residues treated with laccase. Laccase was used to degrade lignin from ginseng residues in order to increase the yield of sugars. Reaction conditions, including solid loading, pH, enzyme concentration, incubation temperature, and incubation time, were investigated and optimized. The results showed that the optimum conditions were 20% of solid loading (w/v), pH 7, 300 IU/ml, temperature of 40 degrees C and incubation time of 6 h. The minimum residual lignin obtained was 59.89%. The results also showed that 56.58% sugars including 12.04% water soluble polysaccharides (WSP), 16.24% water insoluble polysaccharides (WIP) and 5.08% reducing sugar were afforded from delignify substance. Chemical characters of these sugars were analyzed. Pretreat of laccase delignification for sugars production is expected to be applied to other herbal residues.