期刊
BIORESOURCE TECHNOLOGY
卷 307, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2020.123208
关键词
Aspergillus terreus; Bamboo residues; Itaconic acid; Undetoxified enzymatic hydrolysate; High titer
资金
- Biomass Energy and Material, Jiangsu Province [JSBEM-S202011]
- National Natural Science Foundation of China [31770636]
Itaconic acid (IA) production by fermentation of undetoxified hydrolysate of bamboo residues by Aspergillus terreus was demonstrated. Monosaccharides were obtained by pretreatment and enzymatic hydrolysis of bamboo residues. A. terreus could not grow and synthesize IA in the hydrolysate. The buffer was confirmed to be an inhibitor, and was successfully replaced by deionized water as the suspension, to release equivalent sugar and eliminate the inhibition. Corn steep liquor significantly improved the adaptability of A. terreus to the hydrolysate at 2.0 g/L. The IA titer obtained (19.35 g/L IA) was the highest to be reported for IA production from lignocellulose without detoxification. Simultaneous saccharification and fermentation and fed-batch fermentation increased the titer to 22.43 g/L and 41.54 g/L, respectively. Meanwhile, economic assessment proved that bamboo residues were potential substrates for IA production with economic effectiveness.
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