期刊
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
卷 1868, 期 1, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.bbamcr.2020.118823
关键词
Microfluidics; Biomolecular condensates; Liquid-liquid phase separation; Proteins and RNAs; Membraneless organelles; Droplet compartments
资金
- Swiss National Science Foundation [205321_179055]
- Synapsis Foundation for Alzheimer's Disease
- ETH Research Grant [ETH-33 17-2]
- Swiss National Science Foundation (SNF) [205321_179055] Funding Source: Swiss National Science Foundation (SNF)
The increasing evidence shows that membraneless organelles are essential in cellular organization, and the physico-chemical rules underlying their assembly and functions still need further exploration. Microfluidic technologies are attractive tools for analyzing biomolecular phase transitions, providing high-throughput measurements and precise control over self-assembly in time and space.
An increasing body of evidence shows that membraneless organelles are key components in cellular organization. These observations open a variety of outstanding questions about the physico-chemical rules underlying their assembly, disassembly and functions. Some molecular determinants of biomolecular condensates are challenging to probe and understand in complex in vivo systems. Minimalistic in vitro reconstitution approaches can fill this gap, mimicking key biological features, while maintaining sufficient simplicity to enable the analysis of fundamental aspects of biomolecular condensates. In this context, microfluidic technologies are highly attractive tools for the analysis of biomolecular phase transitions. In addition to enabling high-throughput measurements on small sample volumes, microfluidic tools provide for exquisite control of self-assembly in both time and space, leading to accurate quantitative analysis of biomolecular phase transitions. Here, with a specific focus on droplet-based microfluidics, we describe the advantages of microfluidic technology for the analysis of several aspects of phase separation. These include phase diagrams, dynamics of assembly and disassembly, rheological and surface properties, exchange of materials with the surrounding environment and the coupling between compartmentalization and biochemical reactions. We illustrate these concepts with selected examples, ranging from simple solutions of individual proteins to more complex mixtures of proteins and RNA, which represent synthetic models of biological membraneless organelles. Finally, we discuss how this technology may impact the bottom-up fabrication of synthetic artificial cells and for the development of synthetic protein materials in biotechnology.
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