期刊
BIOCHEMICAL JOURNAL
卷 477, 期 15, 页码 2807-2820出版社
PORTLAND PRESS LTD
DOI: 10.1042/BCJ20200408
关键词
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资金
- DPST Royal Government of Thailand Scholarship
- Industrial Biotechnology Catalyst Grant (Innovate UK) [BB/M018261/1]
- Industrial Biotechnology Catalyst Grant (BBSRC) [BB/M018261/1]
- Industrial Biotechnology Catalyst Grant (EPSRC) [BB/M018261/1]
- BBSRC IAA Follow-on-Fund award [BBSRC IAA BB/S506709/1]
- BBSRC [BB/M018261/1, BB/R017689/1]
- BBSRC [BB/M018261/1, BB/R017689/1] Funding Source: UKRI
The Escherichia coli NarX/NarL two-component response-regulator system regulates gene expression in response to nitrate ions and the NarL protein is a global transcription factor, which activates transcript initiation at many target promoters. One such target, the E. coli ogt promoter, which controls the expression of an O-6-alkylguanine-DNA-alkyltransferase, is dependent on NarL binding to two DNA targets centred at positions -44.5 and -77.5 upstream from the transcript start. Here, we describe ogt promoter derivatives that can be activated solely by NarL binding either at position -44.5 or position -77.5. We show that NarL can also activate the ogt promoter when located at position -67.5. We present data to argue that NarL-dependent activation of transcript initiation at the ogt promoter results from a direct interaction between NarL and a determinant in the C-terminal domain of the RNA polymerase alpha subunit. Footprinting experiments show that, at the -44.5 promoter, NarL and the C-terminal domain of the RNA polymerase alpha subunit bind to opposite faces of promoter DNA, suggesting an unusual mechanism of transcription activation. Our work suggests new organisations for activator-dependent transcription at promoters and future applications for biotechnology.
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