Article
Microbiology
Yunpu Jia, Nadia A. Samak, Xuemi Hao, Zheng Chen, Qifeng Wen, Jianmin Xing
Summary: This study introduces a multigene fusion strategy to enhance the accessibility and degradation performance of PETase in degrading PET. The hydrophobic cell surface display system constructed in Escherichia coli shows improved PETase interaction with PET, leading to higher degradation rate and stability. The HCSD system also modifies the PET surface, making it rougher and more hydrophilic, enhancing the overall degradation process.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Engineering, Environmental
Jing Ji, Tianpeng Gao, El-Sayed Salama, Marwa M. El-Dalatony, Liang Peng, Yuxing Gong, Pu Liu, Xiangkai Li
Summary: This study developed a novel whole-cell biocatalyst to accelerate the degradation of antibiotics in pharmaceutical wastewater. By displaying β-lactamase on the cell surface, the biocatalyst showed high enzyme activity and stable performance, significantly improving antibiotic removal and overall reactor performance.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
Wenhan Yang, Mingjia Li, Meiqi Wu, Shiqin Yu, Jingwen Zhou
Summary: The purified (R)-Carbonyl reductase (RCR) can be immobilized on the surface of E. coli using the Lpp-OmpA and EhaA anchors, and the Lpp-OmpA shows better efficacy in displaying the target molecules. By deleting the NAD+ degrading enzyme UshA, the whole-cell biocatalytic activity is enhanced. The created biocatalyst can retain a high catalytic activity after multiple recycling.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Environmental Sciences
Shuang Wang, Zuodeng Sun, Chuanbo Ren, Fei Li, Yingjiang Xu, Huifeng Wu, Chenglong Ji
Summary: This study explored the time- and dose-dependent effects of TBBPA on detoxification and reproductive endocrine disruption in Mytilus galloprovincialis. The results showed that the accumulation of TBBPA increased linearly with exposure time and dose. The cytochrome P450 family 3 (CYP3A1-like) and glutathione-S-transferase (GST) were involved in the biotransformation of TBBPA. The up-regulation of sulfotransferases (SULTs) and steroid sulfatase (STS) suggested their involvement in TBBPA detoxification and reproductive endocrine disruption.
MARINE ENVIRONMENTAL RESEARCH
(2023)
Article
Agricultural Engineering
Xijun Wang, Xiao Feng, Guixian Chen, Baofeng Lin, Haisong Qi
Summary: This study investigated the features and potential application of sulfated cellulose nanofibrils in nanoparticle dispersion. The results showed that the obtained sulfated cellulose nanofibrils had high yield and specific width and length. The properties of the nanofibrils could be tailored by adjusting the reaction conditions. Furthermore, the sulfated cellulose nanofibrils exhibited excellent dispersion properties for nanoparticles.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Engineering, Environmental
Xuhui Bian, Daoyuan Chen, Lei Han
Summary: The study introduced a portable assay device for on-site pesticides detection with functions of synchronous pretreatment, rapid colorimetric assay, and detoxication, by utilizing microbial surface display technology and biomineralization techniques. The device showed promising performance in real-time detection of organophosphorus pesticides with enhanced hydrolase activity and catalytic reduction activity of M-OPH, demonstrating its potential for practical applications in pesticide detection.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Nanoscience & Nanotechnology
Alexander J. Winton, Mark A. Allen
Summary: Phage display is used to identify polypeptides binding to lithium-ion battery materials. Output/input assays and transmission electron microscopy are employed for quantitative measure and verification. Isothermal titration calorimetry and alanine scanning determine binding residues and affinity, and a bifunctional peptide is validated for stability and potential application in lithium-ion battery electrodes.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biotechnology & Applied Microbiology
Tea Martinic Cezar, Mateja Lozancic, Ana Novacic, Ana Maticevic, Dominik Matijevic, Beatrice Vallee, Vladimir Mrsa, Renata Teparic, Bojan Zunar
Summary: By studying yeast surface display, we have developed a new tool that can efficiently convert enzymes of interest into their surface-displayed forms and anchor them near the cell wall. Through rational engineering, we have designed multiple fusion proteins and discovered guidelines for efficient surface display. Additionally, we have developed a small peptide tag that promotes efficient surface display.
MICROBIAL CELL FACTORIES
(2023)
Article
Microbiology
Shuo Yang, Junfeng Shen, Jiliang Deng, Hongxing Li, Jianzhi Zhao, Hongting Tang, Xiaoming Bao
Summary: This study focuses on improving the production of recombinant proteins in yeast cell factories by modifying cell polarization. Overexpression of genes related to cell polarization can enhance the surface display and secretion of recombinant proteins. Combinatorial modification of gene expression can increase protein production in a dose-dependent manner.
Article
Chemistry, Analytical
Yuan Ding, He Chen, Jiao Li, Lianrun Huang, Guangyue Song, Zhenfeng Li, Xiude Hua, Gualberto Gonzalez-Sapienza, Bruce D. Hammock, Minghua Wang
Summary: By optimizing the phage display system and introducing covalent attachment of tracer molecules, a dual display system utilizing phages was successfully developed for phage bioluminescent immunoassay (P-BLEIA) for detecting 2,4-D. The method showed a significant improvement in detection sensitivity and accuracy compared to traditional enzyme-linked immunosorbent assay (ELISA), with 16-fold and 8-fold enhancements in limit of detection and 50% inhibition concentration, respectively.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Mengmeng Zheng, Jianmin Gao
Summary: Peptides, as a therapeutic modality between small molecules and antibodies, are gaining attention for their ability to combine the benefits of both. This study presents a novel phage library that incorporates two distinct designer groups, allowing the screening of peptides with diverse and non-natural functional motifs. The modification of phage-displayed peptides through sequential chemical reactions resulted in the discovery of potent peptide inhibitors against TEV protease.
ACS CHEMICAL BIOLOGY
(2023)
Article
Microbiology
Dongping Zhao, Chan Li, Haoqiang Jiang, Yuqing Yin, Changjing Zhou, Haiming Huang, Yunkun Qi, Lei Li
Summary: Tyrosine sulfation plays a vital role in biochemical reactions, but the lack of effective probes has hindered its study. This study combined a novel selection scheme and next-generation sequencing (NGS) data analysis to successfully identify sTyr superbinders, using the SH2 domain as a template.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Mariana Vendrasco Tacin, Tales A. Costa-Silva, Ariela Veloso de Paula, Jose M. Palomo, Valeria de Carvalho Santos-Ebinuma
Summary: The study focused on the immobilization of lipase produced by Aspergillus sp. through hydrophobic interaction, which showed increased stability and reusability. Immobilization on octyl-sepharose (C-8) resulted in 22% initial activity, with 151.32% yield and 136% increase in specific activity after 4 consecutive cycles of immobilization. The immobilized lipase maintained 75% of initial activity after 20 cycles, demonstrating its potential for industrial applications.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2021)
Article
Veterinary Sciences
Qiu Xu, Wei Zhou, Shihao Ding, Yao Lu, Songyan Liu, Jun Cao, Zhuo Zhang, Siguo Liu, Shenye Yu
Summary: Johne's disease is a chronic granulomatous enteritis of ruminants caused by Mycobacterium avium subsp. paratuberculosis, resulting in significant economic losses in the livestock industry globally. Developing a surface-display vaccine can stimulate high levels of antibodies in mice and increase CD4+ and CD8+ T cell counts, effectively providing protective immunity against MAP.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Plant Sciences
Yuqi Wang, Shaohua Yang, Chune Li, Taijiao Hu, Siyu Hou, Qing Bai, Xiyue Ji, Feng Xu, Chongdai Guo, Min Huang, Yanfei Cai, Jiping Liu
Summary: In this study, the function of OsNIP1;2, a gene in rice, in aluminum detoxification was characterized. OsNIP1;2 was found to be specifically induced by aluminum stress in the roots and played a role in internal aluminum detoxification by regulating aluminum accumulation in the cell wall, cytosol, and distribution between roots and above-ground tissues.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)