Article
Cardiac & Cardiovascular Systems
Hanqiang Deng, Martin A. Schwartz
Summary: High FSS inhibits endothelial Smad2/3 activation and EndMT in response to inflammatory mediators, resulting in selective EndMT at athero-susceptible, low FSS regions of arteries.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2022)
Article
Hematology
Yeping Li, Huiqi Zhu, Wanghao Xin, Jiaoyan Wang, Chao Yan, Kejing Ying
Summary: In this study, it was found that GDF15 promotes the occurrence of EndMT through the Smad2/p-smad2/snail pathway and reduces the anticoagulation and antifibrinolytic ability of HUVECs. Inhibition of GDF15 expression can alleviate the EndMT process, further improving the coagulation and fibrinolytic function of endothelial cells and reducing the fiber deposition of venous thrombi.
THROMBOSIS JOURNAL
(2023)
Article
Medicine, Research & Experimental
Mingwei Zhu, Jiamei Niu, Jian Jiang, Tianxiu Dong, Yaodong Chen, Xiuhua Yang, Pengfei Liu
Summary: This study found that CHE can effectively inhibit the proliferation, stemness, migration, and invasion of GBM cells and induce apoptosis. Furthermore, CHE can inhibit the progression of GBM by suppressing the TGFB1-ERK1/2/Smad2/3-Snail/ZEB1 signaling pathway.
Article
Oncology
Jingyuan Chen, Shengbo Han, Jinhuang Chen, Ping Hu, Zhu Zeng, Yuhang Hu, Hewei Xiong, Zunxiang Ke, Ya Zhang, Fengyu Xu, Gang Zhao
Summary: Research findings suggest that the acidic microenvironment promotes EndMT of HUVECs through the miR-548ac/YB-1/Snail axis, potentially contributing to pancreatic cancer metastasis. Under acidic conditions, HUVECs underwent mesenchymal transition with increased migration, permeability, and expression of alpha-SMA and Vimentin, while the expression of VE-cadherin and CD31 decreased. Furthermore, miRNA-548ac expression significantly decreased in acidity-treated HUVECs.
CANCER CELL INTERNATIONAL
(2021)
Article
Cell Biology
Rafael Cierpka, Ralf Weiskirchen, Anastasia Asimakopoulos
Summary: Understanding the molecular mechanisms of hepatic fibrogenesis is crucial for treatment development, with hepatic stellate cells (HSC) and Perilipin 5 (PLIN5) playing important roles in the pathophysiology. PLIN5 overexpression inhibits HSC activation by suppressing TGF-β1 signaling pathways, indicating its potential as a therapeutic target.
Article
Biochemistry & Molecular Biology
Yujie Li, Yufeng Zhang, Hongshuo Shi, Xuemei Liu, Zifa Li, Jiayi Zhang, Xiuge Wang, Wenbo Wang, Xiaolin Tong
Summary: This study demonstrated that CRTC2 activates the EMT process via the CREB-Smad2/3 signaling pathway in diabetic renal tubules.
MOLECULAR MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Lihong Lu, Ziwen Zhong, Jiahui Gu, Ke Nan, Minmin Zhu, Changhong Miao
Summary: The study indicated that ets1 cooperated with KMT5A to transcribe PFN2, contributing to hyperglycemia-induced EndMT in the glomerular endothelial cells of DN patients and rats.
MOLECULAR MEDICINE
(2021)
Article
Plant Sciences
Ruixue Dong, Xi Zhang, Yadi Liu, Tingting Zhao, Zhongyan Sun, Peiyu Liu, Qian Xiang, Jianfeng Xiong, Xinwen Du, Xifei Yang, Dingkun Gui, Youhua Xu
Summary: This study aimed to investigate the renoprotective effect of Rutin in diabetic kidney disease (DKD) and explore its potential molecular mechanisms. The results showed that Rutin can improve kidney function and structure in DKD by restoring autophagy through inhibiting HDAC1 via the PI3K/AKT/mTOR pathway, thereby alleviating endothelial-to-mesenchymal transition (EndMT). These findings have important implications for the treatment and prevention of DKD.
Article
Pharmacology & Pharmacy
Ye Tian, Zhenhua Bi, Shuaina Ge, Bo Ye, Wenjie Han
Summary: This study reveals the involvement of endothelial-to-mesenchymal transition (EndMT) in diabetic nephropathy (DN) and its connection with epithelial-to-mesenchymal transition. By modulating the levels of ELTD1, STAT5A participates in high glucose-mediated EndMT in DN.
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Ning Zhu, Liuyan Zhu, Bingwu Huang, Wenjun Xiang, Xuyong Zhao
Summary: This study found that Gal-3 inhibition can improve myocardial apoptosis, oxidative stress, release of inflammatory cytokines, macrophage infiltration, and fibrosis, thereby reducing cardiac dysfunction in DCM mice. In addition, silencing Gal-3 can also suppress macrophage infiltration and release of inflammatory cytokines induced by high glucose. Finally, a Gal-3/NF-kappa B p65 regulatory network was clarified in the pathogenesis of DCM.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Cell Biology
Liwen Zhang, Zijing Zhu, Huiwen Yan, Wen Wang, Zhenzhen Wu, Fei Zhang, Qixiang Zhang, Guizhi Shi, Junfeng Du, Huiyun Cai, Xuanxuan Zhang, David Hsu, Pu Gao, Hai-long Piao, Gang Chen, Pengcheng Bu
Summary: The study found that creatine promotes colorectal and breast cancer metastasis and shortens mouse survival. By inhibiting GATM or MPS1, cancer metastasis can be reduced and mouse survival can be extended.
Article
Chemistry, Multidisciplinary
Shu Song, Yue Ding, Guo-liang Dai, Yue Zhang, Meng-ting Xu, Jie-ru Shen, Ting-ting Chen, Yun Chen, Guo-liang Meng
Summary: The study showed that reduced expression of SIRT3 in diabetic cardiomyopathy worsens cardiac dysfunction, myocardial injury, ROS accumulation, necroptosis, and inflammation, ultimately exacerbating the condition. This suggests SIRT3 could be a potential molecular target for preventing and treating diabetic cardiomyopathy.
ACTA PHARMACOLOGICA SINICA
(2021)
Article
Pharmacology & Pharmacy
Shengchuan Cao, Qiuhuan Yuan, Qianqian Dong, Xilong Liu, Weikang Liu, Xiaoxuan Zhai, Chuanxin Zhang, Han Liu, Mengxiong Tang, Shujian Wei, Yuguo Chen
Summary: This study reveals the important role of the ALK7-Smad2/3 pathway in vascular smooth muscle cells apoptosis in diabetic atherosclerosis and suggests it as a potential therapeutic target.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Fang-fang Wang, Jia-li Zhang, Yuan Ji, Xue-jiao Yana, Ling Sun, Yi Zhu, Hong Jin
Summary: "DOM induction leads to EndMT and osteogenic differentiation in HUVECs, which can be alleviated by the BDNF/TrkB pathway. DOM enhances glycolysis and inhibits the BDNF/TrkB pathway in HUVECs. BDNF preserves KLF2 and downregulates HK1 in HUVECs after DOM treatment. Mechanistically, increased KLF2 mitigates HK1-mediated glucose metabolism abnormalities. Cell experiments demonstrate that HK1 knockdown or targeted glycolysis inhibitors can suppress EndMT, apoptosis, inflammation, and vascular calcification in HUVECs after DOM exposure. These findings suggest that KLF2 mediates the inhibitory effect of BDNF on diabetic vascular calcification by inhibiting HK1-induced glucose metabolism reprogramming and the EndMT process."
CELLULAR SIGNALLING
(2022)
Article
Cardiac & Cardiovascular Systems
Leigang Jin, Leiluo Geng, Lei Ying, Lingling Shu, Kevin Ye, Ranyao Yang, Yan Liu, Yao Wang, Yin Cai, Xue Jiang, Qin Wang, Xingqun Yan, Boya Liao, Jie Liu, Fuyu Duan, Gary Sweeney, Connie Wai Hong Woo, Yu Wang, Zhengyuan Xia, Qizhou Lian, Aimin Xu
Summary: This study investigates the role of FGF21 signaling in mediating the benefits of exercise on diabetic cardiomyopathy (DCM). The results suggest that the FGF21-SIRT3 axis plays a crucial role in preserving mitochondrial integrity and may serve as a potential therapeutic target for DCM.
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)