期刊
AQUACULTURE
卷 531, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aquaculture.2020.735780
关键词
Flavobacterium columnare; RPA; LFD; On-site detection
资金
- Chulalongkorn University Ratchadaphiseksomphot Endowment Fund for Postdoctoral Fellowship, Chulalongkorn University Ratchadaphiseksomphot Endowment Fund [CUGR_ 61_013_31_003]
- Fish Infectious Diseases Research Unit (FID RU), Faculty of Veterinary Science, Chulalongkorn University
- CGIAR Research Program on Fish Agri-Food Systems (FISH) led by WorldFish
A new method for detecting F. columnare, using a combination of RPA and LFD technologies, has been developed with high efficiency, speed, and accuracy. The method shows no cross-reactivity with other bacteria and is suitable for field application and low-resource settings.
Flavobacterium columnare is the causative agent of columnaris, a serious disease affecting numerous freshwater fish species worldwide. Although several molecular protocols have been established to detect this pathogen, there still remains a need to develop simpler on-field applicable techniques. Here, we report a more practical and efficient assay to detect F. columnare based on the combination of recombinase polymerase amplification (RPA) and a lateral flow dipstick (LFD). The assay, as performed for 30 min at 37 degrees C for RPA, followed by 2 min at ambient temperature for LFD, revealed a comparable detection limit (200 fg total DNA and 0.4 CFU) to the PCR assay developed by Mabrok et al. in 2020 but required only similar to 35 min to complete tests (i.e. similar to four times faster). Cross-reactivity against other bacteria and false results on 20 clinical samples were not observed, indicating its extremely high specificity and accuracy. The robust performance of the assay to crude tissue samples and clinical specimens reflect its potential use in field application/low-resource settings. The use of a fast and affordable DNA extraction kit is imperative as part of our assay to make the detection of F. columnare more feasible.
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