期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 104, 期 18, 页码 7915-7925出版社
SPRINGER
DOI: 10.1007/s00253-020-10800-y
关键词
Yeast; Saccharomyces cerevisiae; Mitochondrial branched-chain amino acid aminotransferase Bat1; Branched-chain amino acids; Fusel alcohols; Beer brewing
资金
- Japan Society for the Promotion of Science (JSPS) [19K21144]
- Project of the NARO Bio-oriented Technology Research Advancement Institution (Research program on development of innovative technology) [30017B]
- NIH [T32 HG00035]
- National Science Foundation [DBI-0939454, 1516330]
- Faculty Scholar grant from the Howard Hughes Medical Institute
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1516330] Funding Source: National Science Foundation
In the yeastSaccharomyces cerevisiae, the mitochondrial branched-chain amino acid (BCAA) aminotransferase Bat1 plays an important role in the synthesis of BCAAs (valine, leucine, and isoleucine). Our upcoming study (Large et al. bioR chi iv. 10.1101/2020.06.26.166157, Large et al.2020) will show that the heterozygous tetraploid beer yeast strain, Wyeast 1056, which natively has a variant causing one amino acid substitution of Ala234Asp in Bat1 on one of the four chromosomes, produced higher levels of BCAA-derived fusel alcohols in the brewer's wort medium than a derived strain lacking this mutation. Here, we investigated the physiological role of the A234D variant Bat1 inS. cerevisiae. Bothbat1 increment andbat1(A234D)cells exhibited the same phenotypes relative to the wild-type Bat1 strain-namely, a repressive growth rate in the logarithmic phase; decreases in intracellular valine and leucine content in the logarithmic and stationary growth phases, respectively; an increase in fusel alcohol content in culture medium; and a decrease in the carbon dioxide productivity. These results indicate that amino acid change from Ala to Asp at position 234 led to a functional impairment of Bat1, although homology modeling suggests that Asp234 in the variant Bat1 did not inhibit enzymatic activity directly.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据