Catalytically-active inclusion bodies for biotechnology—general concepts, optimization, and application

Detailed small-scale characterization and scale-up of active YFP inclusion body production with Escherichia coli induced by a tetrameric coiled coil domain

(2020) Robin Lamm et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Comparison of Enzymes Immobilised on Immobeads and Inclusion Bodies: A Case Study of a Trehalose Transferase

(2020) Luuk Mestrom et al.   ChemCatChem

Tailoring the properties of (catalytically)-active inclusion bodies

(2019) V. D. Jäger et al.   Microbial Cell Factories

Broadening the Scope of Biocatalysis in Sustainable Organic Synthesis

(2019) Roger A. Sheldon et al.   ChemSusChem

New trends in aggregating tags for therapeutic protein purification

(2018) Xiaofeng Yang et al.   BIOTECHNOLOGY LETTERS

The limits to biocatalysis: pushing the envelope

(2018) Roger A. Sheldon et al.   CHEMICAL COMMUNICATIONS

Comparative study of the insoluble and soluble Ulp1 protease constructs as Carrier free and dependent protein immobilizates

(2018) Li Jiang et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Catalytically active inclusion bodies of L-lysine decarboxylase from E. coli for 1,5-diaminopentane production

(2018) Ramona Kloss et al.   Scientific Reports

Magnetization of active inclusion bodies: comparison with centrifugation in repetitive biotransformations

(2018) Romana Koszagova et al.   Microbial Cell Factories

Direct production of a genetically-encoded immobilized biodiesel catalyst

(2018) Bradley S. Heater et al.   Scientific Reports

A new approach to obtain pure and active proteins from Lactococcus lactis protein aggregates

(2018) L. Gifre-Renom et al.   Scientific Reports

A Synthetic Reaction Cascade Implemented by Colocalization of Two Proteins within Catalytically Active Inclusion Bodies

(2018) Vera D. Jäger et al.   ACS Synthetic Biology

Catalytically-active inclusion bodies—Carrier-free protein immobilizates for application in biotechnology and biomedicine

(2017) Ulrich Krauss et al.   JOURNAL OF BIOTECHNOLOGY

Cadaverine Production by Using Cross-Linked Enzyme Aggregate of Escherichia coli Lysine Decarboxylase

(2017) Se Hyeon Park et al.   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

A review on Lactococcus lactis: from food to factory

(2017) Adelene Ai-Lian Song et al.   Microbial Cell Factories

Fibril structure of amyloid-β(1–42) by cryo–electron microscopy

(2017) Lothar Gremer et al.   SCIENCE

Bacterial Inclusion Bodies: Discovering Their Better Half

(2017) Ursula Rinas et al.   TRENDS IN BIOCHEMICAL SCIENCES

A cleavable self-assembling tag strategy for preparing proteins and peptides with an authentic N-terminus

(2017) Qing Zhao et al.   Biotechnology Journal

Functional protein-based nanomaterial produced in microorganisms recognized as safe: A new platform for biotechnology

(2016) Olivia Cano-Garrido et al.   Acta Biomaterialia

Recombinant production of medium- to large-sized peptides in Escherichia coli using a cleavable self-aggregating tag

(2016) Qing Zhao et al.   Microbial Cell Factories

Recombinant production of influenza hemagglutinin and HIV-1 GP120 antigenic peptides using a cleavable self-aggregating tag

(2016) Wanghui Xu et al.   Scientific Reports

Biotransformation of lysine into cadaverine using barium alginate-immobilized Escherichia coli overexpressing CadA

(2015) Shashi Kant Bhatia et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Fusion of a Coiled-Coil Domain Facilitates the High-Level Production of Catalytically Active Enzyme Inclusion Bodies

(2015) Martin Diener et al.   ChemCatChem

New ways to boost molecular dynamics simulations

(2015) Elmar Krieger et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

Development of engineered Escherichia coli whole-cell biocatalysts for high-level conversion of l-lysine into cadaverine

(2015) Young Hoon Oh et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Production of functional inclusion bodies in endotoxin-free Escherichia coli

(2014) Fabián Rueda et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Soni-removal of nucleic acids from inclusion bodies

(2014) Muniasamy Neerathilingam et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

YASARA View—molecular graphics for all devices—from smartphones to workstations

(2014) Elmar Krieger et al.   BIOINFORMATICS

Characterization of an extremely thermostable but cold-adaptive β-galactosidase from the hyperthermophilic archaeon Pyrococcus furiosus for use as a recombinant aggregation for batch lactose degradation at high temperature

(2014) Qing Dong et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

From zero to hero – Production of bio-based nylon from renewable resources using engineered Corynebacterium glutamicum

(2014) Stefanie Kind et al.   METABOLIC ENGINEERING

The myxobacterial metabolite ratjadone A inhibits HIV infection by blocking the Rev/CRM1-mediated nuclear export pathway

(2014) Eric Fleta-Soriano et al.   Microbial Cell Factories

Minireactor-based high-throughput temperature profiling for the optimization of microbial and enzymatic processes

(2014) Martin Kunze et al.   Journal of Biological Engineering

Enzyme immobilisation in biocatalysis: why, what and how

(2013) Roger A. Sheldon et al.   CHEMICAL SOCIETY REVIEWS

Self-assembling amphipathic alpha-helical peptides induce the formation of active protein aggregates in vivo

(2013) Zhanglin Lin et al.   FARADAY DISCUSSIONS

Active inclusion bodies of acid phosphatase PhoC: aggregation induced by GFP fusion and activities modulated by linker flexibility

(2013) Ziliang Huang et al.   Microbial Cell Factories

Rational Engineering of Recombinant Picornavirus Capsids to Produce Safe, Protective Vaccine Antigen

(2013) Claudine Porta et al.   PLoS Pathogens

Functional Inclusion Bodies Produced in Bacteria as Naturally Occurring Nanopills for Advanced Cell Therapies

(2012) Esther Vázquez et al.   ADVANCED MATERIALS

Active inclusion body formation using Paenibacillus polymyxa PoxB as a fusion partner in Escherichia coli

(2012) Soo-Young Park et al.   ANALYTICAL BIOCHEMISTRY

Production, Characterization, and Application of an Organic Solvent-Tolerant Lipase Present in Active Inclusion Bodies

(2012) Suxia Li et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Production of soluble recombinant proteins in Escherichia coli: Effects of process conditions and chaperone co-expression on cell growth and production of xylanase

(2012) Kamna Jhamb et al.   BIORESOURCE TECHNOLOGY

Small surfactant-like peptides can drive soluble proteins into active aggregates

(2012) Bihong Zhou et al.   Microbial Cell Factories

Computer-aided high-throughput cloning of bacteria in liquid medium

(2011) Tuval Yehezkel et al.   BIOTECHNIQUES

Generation of catalytic protein particles in Escherichia coli cells using the cellulose-binding domain from Cellulomonas fimi as a fusion partner

(2011) Su-Lim Choi et al.   BIOTECHNOLOGY AND BIOPROCESS ENGINEERING

Pre-expression of a sulfhydryl oxidase significantly increases the yields of eukaryotic disulfide bond containing proteins expressed in the cytoplasm of E.coli

(2011) Van Nguyen et al.   Microbial Cell Factories

Computational protein design with explicit consideration of surface hydrophobic patches

(2011) Ron Jacak et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Characterization of a synthetic bacterial self-destruction device for programmed cell death and for recombinant proteins release

(2011) Lorenzo Pasotti et al.   Journal of Biological Engineering

Phosphorothioate-based ligase-independent gene cloning (PLICing): An enzyme-free and sequence-independent cloning method

(2010) Milan Blanusa et al.   ANALYTICAL BIOCHEMISTRY

Automated seamless DNA co-transformation cloning with direct expression vectors applying positive or negative insert selection

(2010) Natacha Olieric et al.   BMC BIOTECHNOLOGY

Isolation of cell-free bacterial inclusion bodies

(2010) Escarlata Rodríguez-Carmona et al.   Microbial Cell Factories

Guidelines and Cost Analysis for Catalyst Production in Biocatalytic Processes

(2010) Pär Tufvesson et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

Structure of Concatenated HAMP Domains Provides a Mechanism for Signal Transduction

(2010) Michael V. Airola et al.   STRUCTURE

Surface Cell Growth Engineering Assisted by a Novel Bacterial Nanomaterial

(2009) Elena García-Fruitós et al.   ADVANCED MATERIALS

Robo-Lector – a novel platform for automated high-throughput cultivations in microtiter plates with high information content

(2009) Robert Huber et al.   Microbial Cell Factories

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Enzymatic synthesis of sialylation substrates powered by a novel polyphosphate kinase (PPK3)

(2009) Jozef Nahálka et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Fourier transform infrared spectroscopy analysis of the conformational quality of recombinant proteins within inclusion bodies

(2008) Silvia Maria Doglia et al.   Biotechnology Journal

A crosslinked inclusion body process for sialic acid synthesis

(2008) Jozef Nahálka et al.   JOURNAL OF BIOTECHNOLOGY

Size characterization of inclusion bodies by sedimentation field-flow fractionation

(2008) Gerd Margreiter et al.   JOURNAL OF BIOTECHNOLOGY

Engineering inclusion bodies for non denaturing extraction of functional proteins

(2008) Špela Peternel et al.   Microbial Cell Factories

A One Pot, One Step, Precision Cloning Method with High Throughput Capability

(2008) Carola Engler et al.   PLoS One

Production of Nonclassical Inclusion Bodies from Which Correctly Folded Protein Can Be Extracted

(2005) Simona Jevševar et al.   BIOTECHNOLOGY PROGRESS