Article
Polymer Science
Karina Oliveira Lima, Cristian Mauricio Barreto Pinilla, Ailen Aleman, M. Elvira Lopez-Caballero, M. Carmen Gomez-Guillen, Pilar Montero, Carlos Prentice
Summary: Chitosan nanoparticles (CNPs) were prepared using the ionic gelation technique and utilized as carriers for a fish protein hydrolysate (CNPH) with bioactive properties. The CNPH exhibited good stability, antioxidant capacity, and antimicrobial activity, and was able to serve as a stabilizer in a food emulsion system.
Article
Food Science & Technology
Fernando Rivero-Pino, Maria C. Millan-Linares, Sergio Montserrat-de-la-Paz
Summary: Bioactive peptides obtained from different food sources have been proven to have various bioactivities, but characterization of these peptides within a protein hydrolysate is challenging. Prediction tools based on molecular features are being developed to assess the likelihood of new sequences being bioactive. While these tools show promise, in vitro/in vivo validation is still necessary before considering a peptide as bioactive.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2023)
Article
Food Science & Technology
Liudmila Sorokina, Anne Rieder, Shiori Koga, Nils Kristian Afseth, Rita De Cassia Lemos Lima, Steven Ray Wilson, Sileshi Gizachew Wubshet
Summary: This study investigated the correlation between chemical fingerprints and bioactivity of poultry by-product protein hydrolysates. Chemical fingerprints were obtained using Fourier-transform infrared spectroscopy and size exclusion chromatography, and the bioactivities were measured in vitro. Regression models based on the fingerprints showed good prediction performance for bioactivity, providing promising tools for quality control in the production of bioactive peptides.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Food Science & Technology
Lucie G. Moens, Elien De Laet, Jean-Claude Van Ceunebroeck, Ann M. Van Loey, Marc E. G. Hendrickx
Summary: The thermal inactivation of PME was studied in three different potato cultivars, revealing the presence of both thermostable and thermolabile enzyme fractions in each cultivar. Innovator may have a lower level of thermostable PME compared to the other cultivars. The inactivation kinetics of PME from Fontane were investigated in detail and represented by a first order model.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemical Research Methods
Marco Mangiagalli, Diletta Ami, Marcella de Divitiis, Stefania Brocca, Tiziano Catelani, Antonino Natalello, Marina Lotti
Summary: This study investigates the effects of short-chain alcohols on the functional and structural responses of immobilized enzyme N-435. It is found that the inactivation process is highly dependent on alcohol concentration and occurs through two different mechanisms.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Chemistry, Physical
Daniel A. Grajales-Hernandez, Eleftheria Diamanti, Riccardo Moro, Susana Velasco-Lozano, Elisabet Pires, Fernando Lopez-Gallego
Summary: In this study, a multienzymatic system was designed to synthesize drugs, and the reaction efficiency and product purity were improved by controlling the spatial organization of enzymes. The results showed that optimal assembly of biocatalysts can alleviate inhibition and enhance reaction rate. Furthermore, integrating a coenzyme regeneration system with the biocatalyst allowed large-scale synthesis of the substrate.
Article
Biochemical Research Methods
Andreia Monique Lermen, Naiara Jacinta Clerici, Dienefer Borchartt Maciel, Daniel Joner Daroit
Summary: Bacillus sp. CL14 crude protease was partially characterized and used to obtain antioxidant whey protein isolate (WPI) hydrolysates. The optimal activity of the protease was observed at pH 9.0 and 60 degrees C, and it was influenced by various cations and anions.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2023)
Article
Engineering, Environmental
Meng Wang, Valerie A. Kickhoefer, Esther H. Lan, Bruce S. Dunn, Leonard H. Rome, Shaily Mahendra
Summary: The study presents a highly efficient method of embedding proteins in silica using vault protein nanocapsules as templates, achieving nearly 100% immobilization efficiency and maintaining high protein activity during the silicification process.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biochemical Research Methods
Jessica Gathercole, Evelyne Maes, Ancy Thomas, Robert Wieliczko, Anita Grosvenor, Stephen Haines, Stefan Clerens, Santanu Deb-Choudhury
Summary: Understanding the functional attributes of meat proteins is crucial for determining their nutritional benefits. Liquid chromatography-mass spectrometry (LC-MS) was used to compare the molecular peptide features during the digestion process of powdered meat and its enzymatically produced hydrolysate. The results showed that the hydrolysate did not undergo extensive digestion, while the powdered meat was digested in both gastric and intestinal phases.
JOURNAL OF PROTEOMICS
(2023)
Article
Food Science & Technology
Ying Sun, Yan Zhou, Yanmei Ren, Jianan Sun
Summary: In order to make full use of Chinese mitten crab processing by-products, sauces were prepared using different parts of the crab. Two processing methods were employed to prepare these sauces. The taste, odor, amino acid content, and volatile compounds of the sauces were analyzed. The results showed that the Maillard reaction enhanced the umami and saltiness of the sauces and produced more volatile compounds with unique flavors and antioxidant capabilities.
Article
Chemistry, Physical
Luisa M. Merz, Luuk M. van Langen, Per Berglund
Summary: This study investigated the effect of different conditions on the stability of SpATA and analyzed the causes of these effects. The results showed that samples exposed to light had a higher tendency for inactivation compared to samples kept in darkness, with TRIS buffer protecting PLP from degradation.
Article
Engineering, Chemical
Qian Zhang, Ru Liu, Margret Geirsdottir, Shiyu Li, Tumi Tomasson, Shanbai Xiong, Xiuchen Li, Maria Gudjonsdottir
Summary: The objective of this study is to effectively denature the autolysis enzymes of C. frondosa while avoiding quality deterioration. The results showed that boiling and steaming could efficiently inactivate the enzymes, but overheating reduced cooking yield and texture quality. Therefore, boiling at 100 degrees C for 45 minutes was suitable for pre-treatment, while steaming at 100 degrees C for at least 30 minutes was preferable for long-term storage and instant food.
Article
Biochemistry & Molecular Biology
Yakov Kipnis, Anissa Ouald Chaib, Anastassia A. Vorobieva, Guangyang Cai, Gabriella Reggiano, Benjamin Basanta, Eshan Kumar, Peer R. E. Mittl, Donald Hilvert, David Baker
Summary: This study explores the customization of the backbone and sequence of a de novo designed protein to create catalysts for a retro-aldolase model reaction. The results show that active and specific catalysts can be designed in this fold and further optimized using directed evolution. This supports the idea that different folds have different amenability to evolution and may explain the distribution of natural enzymes among different folds.
Article
Biochemistry & Molecular Biology
Yakov Kipnis, Anissa Ouald Chaib, Anastassia A. Vorobieva, Guangyang Cai, Gabriella Reggiano, Benjamin Basanta, Eshan Kumar, Peer R. E. Mittl, Donald Hilvert, David Baker
Summary: By customizing the backbone and sequence of a de novo designed protein, active and specific catalysts can be created, with further optimization of activity and stereoselectivity using directed evolution. Different folds have varying inherent amenability to evolution, which could partially explain the distribution of natural enzymes among different folds.
Article
Pharmacology & Pharmacy
Oliwia Degorska, Daria Szada, Agata Zdarta, Wojciech Smulek, Teofil Jesionowski, Jakub Zdarta
Summary: In this study, lipase immobilized from Aspergillus niger by physical immobilization and mixed physical immobilization and ion exchange was used for kinetic resolution of the ketoprofen racemic mixture. The immobilized lipase showed improved activity and stability, and phosphate buffer at pH 7 was found to be the best solvent.
Article
Environmental Sciences
Jaqueline Lessa Maciel, Patricia Orosco Werlang, Daniel Joner Daroit, Adriano Brandelli
WASTE AND BIOMASS VALORIZATION
(2017)
Article
Environmental Sciences
Lisiane Sobuckil, Rodrigo Ferraz Ramosl, Daniel Joner Daroitl
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2017)
Article
Food Science & Technology
Juliana Both Engel, Caroline Heckler, Eduardo Cesar Tondo, Daniel Joner Daroit, Patricia da Silva Malheiros
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2017)
Article
Biochemical Research Methods
Roberta Fontoura, Daniel J. Daroit, Ana Paula F. Correa, Karla S. Moresco, Lucelia Santi, Walter O. Beys-da-Silva, John R. Yates, Jose Claudio F. Moreira, Adriano Brandelli
Article
Biochemistry & Molecular Biology
Kelly Callegaro, Nicoly Welter, Daniel Joner Daroit
PROCESS BIOCHEMISTRY
(2018)
Review
Engineering, Environmental
Kelly Callegaro, Adriano Brandelli, Daniel Joner Daroit
Article
Environmental Sciences
Leandro Pellenz, Fernando Henrique Borba, Daniel Joner Daroit, Manoel Francisco Mendes Lassen, Suzymeire Baroni, Camila Fernanda Zorzo, Raissa Engroff Guimaraes, Fernando Rodolfo Espinoza-Quinones, Daiana Seibert
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2020)
Article
Food Science & Technology
Caroline Heckler, Caroline Marques Maders Silva, Fabiola Ayres Cacciatore, Daniel Joner Daroit, Patricia da Silva Malheiros
LWT-FOOD SCIENCE AND TECHNOLOGY
(2020)
Article
Green & Sustainable Science & Technology
Vera Analise Schommer, Bruno Munchen Wenzel, Daniel Joner Daroit
Article
Environmental Sciences
Lisiane Sobucki, Rodrigo Ferraz Ramos, Elci Gubiani, Gustavo Brunetto, Douglas Rodrigo Kaiser, Daniel Joner Daroit
INTERNATIONAL JOURNAL OF RECYCLING OF ORGANIC WASTE IN AGRICULTURE
(2019)
Article
Biology
Bernardete da Silva Bernardo, Rodrigo Ferraz Ramos, Kelly Callegaro, Daniel Joner Daroit
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
(2019)
Article
Agriculture, Dairy & Animal Science
T. J. Rieger, C. T. de Oliveira, J. Q. Pereira, A. Brandelli, D. J. Daroit
BRITISH POULTRY SCIENCE
(2017)
Article
Biotechnology & Applied Microbiology
Caroline Torres de Oliveira, Tiago Joel Rieger, Daniel Joner Daroit
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2017)
Article
Biology
Caroline T. De Oliveira, Jamile Q. Pereira, Adriano Brandelli, Daniel J. Daroit