期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 59, 期 42, 页码 18380-18385出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202007649
关键词
bioimaging; chemiluminescence imaging; inflammation tracing; NIR-II imaging
资金
- National Key R&D Program of China [2017YFA0207303]
- National Natural Science Foundation of China (NSFC) [21725502, 51961145403]
- Key Basic Research Program of Science and Technology Commission of Shanghai Municipality [17JC1400100]
Chemiluminescence (CL) sensing without external excitation by light and autofluorescence interference has been applied to high-contrast in vitro immunoassays and in vivo inflammation and tumor microenvironment detection. However, conventional CL sensing usually operates in the range of 400-850 nm, which limits the performance of in vivo imaging due to serious light scattering effects and signal attenuation in tissue. To address this challenge, a new type of CL sensor is presented that functions in the second near-infrared window (NIR-II CLS) with a deep penetration depth (approximate to 8 mm). Successive CL resonance energy transfer (CRET) and Forster resonance energy transfer (FRET) from the activated CL substrate to two rationally designed donor-acceptor-donor fluorophores BTD540 and BBTD700 occurs. NIR-II CLS can be selectively activated by hydrogen peroxide over other reactive oxygen species (ROSs). Moreover, NIR-II CLS is capable of detecting local inflammation in mice with a 4.5-fold higher signal-to-noise ratio (SNR) than that under the NIR-II fluorescence modality.
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