Article
Spectroscopy
Yifeng Lan, Qiang He, Yingqi Ma, Yanli Wei, Zhiwen Wei, Chuan Dong
Summary: In this study, a simple and sensitive fluorescent aptasensing system was developed for ATP detection. The system showed desirable sensitivity and detection performance.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Chemistry, Analytical
Nuo Duan, Changxin Li, Mingqian Song, Kexin Ren, Zhouping Wang, Shijia Wu
Summary: Aptamers with high affinity to DON were selected and used to fabricate a sensitive aptasensor for DON detection. The practical application of this method in maize flour samples showed satisfactory recovery.
Article
Energy & Fuels
Yong Sun, Cheng Xu, Yigang Jin, Mengsu Zhu, Ze Li, Zixin Chang, Yimeng Sun, Wei Xu, Daoben Zhu
Summary: A reasonable combination of different materials can produce hybrids with significantly improved properties based on synergistic effects. In this study, highly conductive amorphous Cu3BHT (A-CuBHT) is grown on the surface of 1T(H) phase MoS2 by in situ polymerization, resulting in hybrids with significantly improved electrical conductivity. Furthermore, the 1T phase in MoS2 is stabilized with the cooperation of A-CuBHT. Particularly, the hybrid material containing 50 wt % A-CuBHT exhibits a specific capacitance of 329.9 F g(-1), which is 2.3 times that of pure 1T(H) phase MoS2 (143.7 F g(-1)) and 1.5 times that of A-CuBHT (220.6 F g(-1)). Additionally, the asymmetric supercapacitor device constructed by the material exhibits a power density of 349.7 W kg(-1) and an energy density of 13.2 W h kg(-1).
Article
Chemistry, Physical
Bo Pang, Huiru Yang, Liying Wang, Jiaqi Chen, Lihong Jin, Bingjun Shen
Summary: The key problem in tumor photothermal therapy research is to control the size of nanosheets and reduce the potential toxicity of photothermal nanosheets. MoS2 nanosheets around 10 nm in size were prepared and enhanced biocompatibility was achieved by combining BSA with MoS2 for high specific recognition. The successfully prepared MoS2-BSA-Apt nanosheets demonstrated excellent photostability and photothermal effect, with in vitro and in vivo studies showing good biological safety, efficient tumor cell targeting, and tumor growth inhibitory effects in mice.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2021)
Article
Engineering, Electrical & Electronic
Afsaneh Abareshi, Nasrin Salehi
Summary: A nanoplatform based on MoS2 nanoflakes decorated with super-paramagnetic iron oxide magnetic nanoparticle (Fe3O4) was reported in this paper. The nanoplatform has high surface area and excellent photothermal properties, making it a suitable photothermal agent for biomedical applications.
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
(2022)
Article
Electrochemistry
Amina Rhouati, Jean-Louis Marty, Alina Vasilescu
Summary: The review discusses the powerful tool of combining aptamers' specific recognition abilities and enzymes' catalytic power for highly selective and sensitive detection in bioanalysis. The use of enzymes and DNAzymes as amplification methods, along with the integration of nucleic acid catalysts into aptazymes, are highlighted as preferred approaches in electrochemical aptasensors.
ELECTROCHIMICA ACTA
(2021)
Article
Chemistry, Multidisciplinary
Yiyang Zhao, Wenhao Luo, Huiqing Luo, Xiaodi Liu, Wenjun Zheng
Summary: By preparing a 3D multilevel heterostructure VANS-MoS2-CNTs, the disadvantages of the MoS2 anode for LIBs in terms of low intrinsic conductivity, poor mechanical stability, and adverse reaction with electrolytes are overcome. VANS-MoS2-CNTs exhibit good conductivity, high structured stability, and excellent Li+/electron transport, resulting in high discharge capacity, excellent rate capacity, and good cyclic stability.
Article
Chemistry, Physical
Peng Chen, Pablo Rafael Brito-Parada, Shaoxian Song, Feifei Jia
Summary: The photocatalytic activity of semiconductors can be enhanced by surface decoration of precious metal particles. In this study, a MoS2-based Ohmic contact was fabricated by depositing Ag nanoparticles on MoS2 nanosheets, which improved the photoreduction of Au(I) from a low concentration Au(S2O3)23- solution. Efficient interface charge transfer and higher electron concentration on the surface of Ag/MoS2 NSs resulted in a significantly higher gold recovery rate compared to pristine MoS2 NSs.
APPLIED SURFACE SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Chuangui Sheng, Jian Zhao, Fangzhi Yu, Lele Li
Summary: The enzymatically triggerable, aptamer-based signal amplification (ETA-SA) strategy enables inflammatory cell-specific imaging of ATP through spatially-resolved signal amplification, leading to improved sensitivity and spatial specificity for inflammation-specific ATP imaging in vivo.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Food Science & Technology
Zhiping Yu, Chengqiang Li, Jiashuai Sun, Xia Sun, Guodong Hu, Thierry Noguer
Summary: The accumulation of pesticide residues poses a significant threat to human health and ecological systems. In this study, an electrochemiluminescence aptasensor was developed for the rapid detection of malathion residues. The sensor showed excellent specificity, repeatability, and stability, and was successfully applied to detect pesticide residues in authentic samples.
Article
Nanoscience & Nanotechnology
Gaojian Yang, Yuan Liu, Yan Deng, Zhu Chen, Hui Chen, Song Li, Nongyue He
Summary: This study successfully identified a high-affinity aptamer Cd-1 for the recognition of cadmium ions using a modified SELEX method. Cd-1 showed the highest affinity for cadmium ions and exhibited certain specificity for detecting other heavy metal ions. The strategy of immobilizing an ssDNA library on a matrix is believed to be applicable for the discovery of other ions in a reproducible manner.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2021)
Article
Spectroscopy
Nuo Duan, Changxin Li, Mingqian Song, Zhouping Wang, Changqing Zhu, Shijia Wu
Summary: An aptamer-based optical assay was developed for analyzing Pb2+, a hazardous heavy metal in the food chain harmful to human health, with higher sensitivity and successful detection results.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Analytical
Mingming Xu, Yu Peng, Hualin Yang, Yu Zhou
Summary: In this study, a highly sensitive biosensor based on a Cd2+-specific DNA aptamer and HCR was developed for detecting Cd2+ ions. The biosensor showed excellent selectivity and recovery rates, and had a low detection limit for Cd2+.
Article
Spectroscopy
Yonghui Xuan, Xiang Li, Changling Yan, Gongke Wang
Summary: Due to the complexity and cost of traditional methods for detecting heavy metal pollution in water, there is a need for rapid and simple methods. In this study, a nanobiosensor based on molybdenum disulphide (MoS2) nanosheets and fluorophore labeled oligonucleotides was proposed for detecting Hg2+ or Ag+ ions in water using fluorescence spectroscopy. The sensor works by forming stable double-stranded DNA (dsDNA) structures through the mismatching of single-stranded DNA (ssDNA) rich in thymine (T) or cytosine (C) with Hg2+ or Ag+. The sensor showed high sensitivity and selectivity, with minimum detection limits of 6.8 nM and 8.9 nM for Hg2+ and Ag+ ions, respectively.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Materials Science, Composites
Pengxiang Gao, Jiehao Lei, Jisuan Tan, Guan Wang, Hongxia Liu, Li Zhou
Summary: A simple method for preparing a stable and efficient composite adsorbent for Hg2+ removal is reported. The composite adsorbent exhibited convenient magnetic separation capability, high adsorption rate and capacity, as well as reusability.
COMPOSITES COMMUNICATIONS
(2021)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)