期刊
ANALYTICA CHIMICA ACTA
卷 1134, 期 -, 页码 96-105出版社
ELSEVIER
DOI: 10.1016/j.aca.2020.08.004
关键词
Cu/Au/Pt trimetallic nanoparticles; Microcystin-LR; DNA hydrogel; Signal amplification; Detection
资金
- National Key R&D Program of China [2018YFC1603500, 2017YFC1601101]
- NationalNatural Science Foundation of China [21676305]
Sensitive and reliable analytical methods for monitoring of microcystin-LR (MC-LR) are urgently necessary due to its great harm to human health and aquatic organisms. In this work, a novel Cu/Au/Pt trimetallic nanoparticles (Cu/Au/Pt TNs)-encapsulated DNA hydrogel was prepared for colorimetric detection of MC-LR. The Cu/Au/Pt TNs were captured and released with precise control by the target-responsive 3D DNA hydrogels, which combined dual advantages of the target responsive DNA hydrogel and Cu/Au/Pt TNs of enhanced peroxidase-like activity. The DNA hydrogel network was constructed by hybridizing MC-LR aptamer with two complementary DNA strands on linear polyacrylamide chains. As long as MC-LR presented, the aptamer competitively binds with the MC-LR, causing the hydrogel to dissolve and release the preloaded Cu/Au/Pt TNs which could catalyze the reaction between H2O2 and TMB to produce color changes. In view of this sensitive strategy, this Cu/Au/Pt TNs-encapsulated DNA hydrogel-based colorimetric biosensor can achieve quantitative determination of MC-LR. The results showed that as-proposed colorimetric biosensor could sensitively detect MC-LR with a linear range of 4.0 -10000 ng L-1 and a detection limit of 3.0 ng L-1. This work proved that the sensor had great potential to be applied in MC-LR detection and also provided the opportunity to develop colorimetric biosensor for other targets using this target-responsive and signal-amplification strategy. (C) 2020 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据