期刊
ANALYTICA CHIMICA ACTA
卷 1126, 期 -, 页码 106-113出版社
ELSEVIER
DOI: 10.1016/j.aca.2020.06.009
关键词
Lateral-flow immunoassays; Nanozyme; Streptomycin; Visualization
资金
- National Natural Science Foundation of China [31502118, 31502114]
- Scientific Research Funds in Jiangsu University [13JDG016]
- Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment
Because of the advantages of simplicity, cost-effectiveness and visibility, lateral-flow immunoassays (LFAs) have been widely used in the food safety field. However, the low sensitivity of LFAs needs to be solved. Nanozymes have amazing potential for application in biosensors due to their excellent and abundant enzyme-like characteristics. In this study, an Au@Pt nanozyme synthesized by a one-step method showed the higher affinity with TMB/H2O2 and higher catalytic efficiency than that of horse-radish peroxidase (HRP). For the detection of streptomycin (STR), a typical aminoglycoside antibiotic, a novel LFA based on Au@Pt as a visual tag and an enhanced LFA based on the enzyme-like activity of Au@Pt by addition of the chromogenic substrate 3-amino-9-ethyl-carbazole (AEC) were established and compared with conventional LFA based on AuNPs. The qualitative limit of detection (LOD) was 1 ng mL(-1) for the LFA based on Au@Pt as the visual tag and 0.1 ng mL(-1) for the enhanced LFA based on the activity of Au@Pt, in comparison to 8 ng mL(-1) for LFA based on AuNPs. Furthermore, the level of streptomycin in milk samples from Zhenjiang City was successfully evaluated by the novel LFA based on Au@Pt nanozyme. These results suggest that LFAs based on nanozymes are a promising and effective tool for food safety. (C) 2020 Elsevier B.V. All rights reserved.
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