Article
Chemistry, Analytical
Yujie Liu, Hui Liu, Guanliu Yu, Wenbo Sun, Muhammad Aizaz, Guiwen Yang, Lei Chen
Summary: A highly efficient single-tube detection platform based on RPA and CRISPR reaction system has been developed to rapidly and accurately detect MRSA infection. The platform demonstrates satisfactory selectivity and can achieve detection within 30 minutes. It shows outstanding performance in sensitivity, specificity, and resistance identification.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Dan Zhao, Jiutang Tang, Qin Tan, Xiaohong Xie, Xin Zhao, Dingpei Xing
Summary: In this study, a dual-amplified biosensing method based on CRISPR/Cas13a-triggered Cas12a, namely Cas13a-12a amplification, was developed for miRNA-155 detection. The detection limit of this amplification strategy was as low as 0.35 fM, which was much lower than that of the only Cas13a-based assay. Accurate quantification of miRNA-155 expression levels in different cancer patients was achieved using this strategy. Hence, the developed method provides a supersensitive and highly specific miRNA sensing platform for clinical applications.
Article
Chemistry, Analytical
Xiaoying Cai, Dan Zhao, Xingrong Li, Qingyuan Zheng, Xiaojia Shu, Shijia Ding, Decai Zhang, Yurong Yan
Summary: A fluorometric biosensor based on CRISPR/Cas12a collateral cleavage has been developed for ultrasensitive and specific detection of FEN1 activity. The biosensing platform demonstrates excellent analytical performance and has been successfully applied for FEN1 detection in serums and cell lysates, suggesting potential clinical applications.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biophysics
Wenjun Liu, Xuena Zhu, Ling Li, Shanshan Wang, Chen-Zhong Li, Tingbo Liang
Summary: Activation of Cas13 is dependent on crRNA-target RNA hybridization. This study develops a controlled activation system for Cas13 using N-terminus tagging, which suppresses target dependent activation through a composite SUMO tag. This discovery not only enables Cas13-based protease detection, but also provides a novel strategy for precise multi-component activation of Cas13.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Andrea Bonini, Noemi Poma, Federico Vivaldi, Denise Biagini, Daria Bottai, Arianna Tavanti, Fabio Di Francesco
Summary: This study developed a label-free biosensing assay for the detection of Escherichia coli and Staphylococcus aureus using a CRISPR/Cas12a system coupled with electrochemical impedance spectroscopy. The Cas12a/gRNA system was able to specifically recognize amplicons from different clinical isolates of E. coli and S. aureus with a limit of detection of 3 nM and a short turnaround time approximately of 1.5 h. This is the first biosensing device based on CRISPR/Cas12a label-free impedance assay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Immunology
Jiewei Cui, Minjuan Zhou, Ying Li, Zhixin Liang, Yanqin Li, Ling Yu, Yang Liu, Yuan Liang, Liangan Chen, Changxi Yang
Summary: A quantum dots immunofluorescence biosensor was successfully developed for S. aureus detection, demonstrating high specificity and sensitivity. The biosensor has a low detection limit and can effectively distinguish other pathogens.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Xiaoling Liu, Xinyue Kang, Chao Lei, Wei Ren, Chenghui Liu
Summary: This study demonstrates that a simple ssDNA probe can efficiently block the trans-cleavage activity of the CRISPR-Cas13a system without any modification, under low Mg2+ concentration. The blocked activity can be recovered by specific release of the DNA blocker from crRNA in a target-responsive manner, providing a convenient method for quantifying various biomarkers. This discovery enriches the CRISPR-Cas13a sensing toolbox and greatly expands its application scope.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Pengfei Song, Pengbo Zhang, Ke Qin, Fengxia Su, Kejian Gao, Xin Liu, Zhengping Li
Summary: This study utilizes CRISPR/Cas13a to recognize the unique junction sequence of circRNA and develops a CRISPR/Cas13a induced exponential amplification assay for sensitive and specific detection of circRNA. The designed primers and high amplification efficiency allow for the detection of low concentrations of circRNA in real biological samples.
Article
Chemistry, Analytical
Yao Han, Fan Li, Lan Yang, Xudong Guo, Xue Dong, Mengwei Niu, Yaxuan Jiang, Lin Li, Hao Li, Yansong Sun
Summary: This study presents an immunocapture magnetic bead-enhanced electrochemical biosensor for ultrasensitive SARS-CoV-2 detection based on CRISPR-Cas13a technology. The biosensor uses low-cost and immobilization-free carbon electrodes for electrochemical signal measurement, streptavidin-coated immunocapture magnetic beads to reduce background noise and enhance detection ability, and isothermal amplification methods in the CRISPR-Cas13a system for nucleic acid detection. The biosensor showed a two orders of magnitude increase in sensitivity with the use of magnetic beads. It can detect SARS-CoV-2 with an ultrasensitive ability as low as 1.66 aM and can be flexibly applied to other viruses.
Article
Biophysics
Tian Tian, Zhiqiang Qiu, Yongzhong Jiang, Debin Zhu, Xiaoming Zhou
Summary: This study introduces a highly efficient dual-gene detection technique based on the orthogonal DNA/RNA collateral cleavage mechanism of the Cas12a/Cas13a system. The technique allows simultaneous detection of multiple amplified products and can be implemented on a portable device for accurate infectious disease screening in resource-limited settings.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Microbiology
Zihao Song, Yue Yu, Xinpeng Bai, Yiguo Jia, Jiayi Tian, Kui Gu, Mengyu Zhao, Changyu Zhou, Xiangyu Zhang, Hongning Wang, Yizhi Tang
Summary: The emergence of antibiotic-resistant bacteria poses a threat to public health. The use of broad-spectrum antibiotics can disrupt beneficial gut microbiota and lead to secondary diseases. Therefore, there is a need to develop a new strategy that can specifically kill pathogens without affecting the resident microbiota. In this study, researchers developed a precise bactericidal system using CRISPR-Cas13a targeting endogenous transcripts of Salmonella enterica serovar Typhimurium. The system demonstrated specific killing, growth inhibition, and clearance of S. Typhimurium in mixed microbial flora in vitro. In a mouse infection model, the system significantly reduced S. Typhimurium colonization in the intestinal tract when delivered by a donor Escherichia coli strain. These findings demonstrate the feasibility and efficacy of the designed CRISPR-Cas13a system in selective killing of pathogens and broaden the utility of conjugation-based delivery of bactericidal approaches.
MICROBIOLOGY SPECTRUM
(2022)
Article
Chemistry, Analytical
Decai Zhang, Yurong Yan, Xiaoxue Cheng, Tiantian Yang, Xingrong Li, Shijia Ding, Xiuming Zhang, Wei Cheng
Summary: This study investigated the trans-cleavage activity of CRISPR-Cas12a and revealed the influence of PAM nucleotides on its activation. The enhanced strand displacement mechanism by Cas12a to promote crRNA invasion was also demonstrated. Additionally, a modular CRISPR-Cas12a-based signal reporting system was developed for universal biosensing, enabling the detection of various analytes with high sensitivity and specificity.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biophysics
Woong Heo, Kyungyeon Lee, Sunyoung Park, Kyung-A Hyun, Hyo-Il Jung
Summary: A electrochemical biosensor based on CRISPR/Cas13a was developed for the rapid and sensitive detection of SARS-CoV-2 without the need for nucleic acid amplification. The biosensor showed high sensitivity and a wide detection range, making it suitable for on-site rapid diagnostic testing.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Jae Hoon Jeung, Hyogu Han, Chang Yeol Lee, Jun Ki Ahn
Summary: This study presents a technique using CRISPR/Cas12a for detecting 3'-5' exonuclease activity. The technique allows sensitive detection of Exo III activity and can be used for screening inhibitors.
Article
Biochemistry & Molecular Biology
Young-hoon Kim, Nahye Kim, Ikenna Okafor, Sungchul Choi, Seonwoo Min, Joonsun Lee, Seung-Min Bae, Keunwoo Choi, Janice Choi, Vinayak Harihar, Youngho Kim, Jin-Soo Kim, Benjamin P. Kleinstiver, Jungjoon K. Lee, Taekjip Ha, Hyongbum Henry Kim
Summary: Researchers have developed an improved version of Sniper-Cas9, called Sniper2L, which combines high specificity with retained high activity for efficient and specific genome editing at a large number of target sequences.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)
