Stimulus intensity-dependent recruitment of Nav1 subunits in action potential initiation in nerve terminals of vagal C-fibers innervating the esophagus

We investigated voltage-gated sodium channel (Na(v)1) subunits that regulate action potential initiation in the nerve terminals of vagal nodose C-fibers innervating the esophagus. Extracellular single fiber recordings were made from the nodose C-fibers, with mechanically sensitive nerve terminals in the isolated innervated guinea pig esophagus. Na(v)1 inhibitors were selectively delivered to the tissue-containing nerve terminals. Graded esophageal distention was used for mechanical stimulation. The Na(v)1.7 inhibitor PF-05089771 nearly abolished action potential initiation in response to low levels of esophageal distention but only partially inhibited the response to higher levels of esophageal distention. The PF-05089771-insensitive component of the response progressively increased (up to approximate to 50%) with increasing esophageal distention and was abolished by tetrodotoxin (TTX). In addition to Na(v)1.7, nodose C-fiber [transient receptor potential channel-vanilloid subfamily member 1 (TRPV1)positive] neurons retrogradely labeled from the esophagus expressed mRNA for multiple TTX-sensitive Na(v)1s. The group Na(v)1.1, Na(v)1.2, and Na(v)1.3 inhibitor ICA-121431 inhibited but did not abolish the PF-05089771-insensitive component of the response to high level of esophageal distention. However, combination of ICA121431 with compound 801, which also inhibits Na(v)1.7 and Na(v)1.6, nearly abolished the response to the high level of esophageal distention. Our data indicate that the action potential initiation in esophageal nodose C-fibers evoked by low (innocuous) levels of esophageal distention is mediated by Na(v)1.7. However. the response evoked by higher (noxious) levels of esophageal distention has a progressively increasing Na(v)1.7-independent component that involves multiple TTX-sensitive Na(v)1s. The stimulus intensity-dependent recruitment of Na(v)1s may offer novel opportunities for strategic targeting of Na(v)1 subunits for inhibition of nociceptive signaling in visceral C-fibers. NEW & NOTEWORTHY We report that pharmacologically distinguishable voltage-gated sodium channels (Na(v)1) mediate action potential initiation at low (innocuous) versus high (noxious) intensity of esophageal distention in nerve terminals of vagal nodose C-fibers. Action potential initiation at low intensity is entirely dependent on Na(v)1.7; however, additional tetrodotoxin (TTX)-sensitive Na(v)1s are recruited at higher intensity of distention. This is the first demonstration that Na(v)1s underlying action potential initiation in visceral C-fibers depend on the intensity of the stimulus.