Article
Chemistry, Analytical
Mousumi Baruah, Anal Jana, Niharika Pareek, Shikha Singh, Animesh Samanta
Summary: This article discusses the crucial role of mitochondria in redox signaling and their involvement in the generation of reactive oxygen species (ROS) and oxidative stress. It emphasizes the importance of simultaneous monitoring of multiple organelles and lipid droplets in cells, and introduces a molecular probe that can detect mitochondrial ROS and monitor lysosomal lipid droplets.
Article
Chemistry, Inorganic & Nuclear
Hong Wang, Changli Zhang, Zhiyong Jiang, Li Xu, Zhipeng Liu
Summary: In this study, phenothiazine-fused boron complexes were identified as promising scaffolds for ratiometric fluorescent hypochlorite probes. The synthesized complexes demonstrated ultrafast and ratiometric absorption/fluorescence changes for hypochlorite. Additionally, an endoplasmic reticulum-targeting probe was developed and showed excellent real-time imaging ability for both endogenous and exogenous hypochlorite in living cells.
DALTON TRANSACTIONS
(2023)
Article
Chemistry, Multidisciplinary
Beidou Feng, Kui Wang, Zhe Wang, Huiyu Niu, Ge Wang, Yuehua Chen, Hua Zhang
Summary: A mitochondrial-targeted probe MBI-OMe was developed to specifically detect changes in ferroptosis-induced ClO-. It showed a ratiometric signal in living cells in response to ferroptosis-induced ClO-.
FRONTIERS IN CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yaheng Li, Shankun Yao, Hongbao Fang, Weijiang He, Yuncong Chen, Zijian Guo
Summary: Zn2+ is essential for physiological processes in the human body and interacts with oxidative stress in pathological tissues. A ratiometric fluorescent Zn2+ probe was designed to detect fluctuations in Zn2+ levels in cells undergoing oxidative stress.
Article
Spectroscopy
Wenjun Bai, Yixuan Li, Li Zhao, Ruxin Li, Jiahou Geng, Yang Lu, Yufen Zhao, Jinhui Wang
Summary: In this study, a ratiometric fluorescent probe based on extended 7C-7C conjugation of a triphenylamine unit was designed for the detection of NTR and lysosomes imaging. The probe showed high selectivity, rapid response, and a wide pH range towards NTR. It also exhibited low cytotoxicity in both normal and tumor cells, as well as excellent lysosomes colocalization.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Lei Wen, Xinyu Ma, Jing Yang, Minmin Jiang, Chao Peng, Zhongyun Ma, Huan Yu, Yinhui Li
Summary: This study proposed a new design strategy based on pi-conjugation modulation for fluorescent probes, which can generate different emission bands in the presence and absence of the target. The newly designed fluorescence probe, Rhod-DCM-B, exhibited outstanding sensitivity and excellent selectivity towards ONOO-. It was successfully employed to determine endogenous and exogenous ONOO- in living cells, and effectively monitored the ONOO- in vivo.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Ting Liu, Lixing Fu, Chenhui Yin, Meng Wu, Ligang Chen, Na Niu
Summary: A smartphone platform utilizing ratiometric fluorescence signals was developed for fast, sensitive, and on-site visual detection of silver ions. By constructing a ratiometric fluorescent probe with FITC and CdTe QDs, the color changes of the system were captured on a portable smartphone platform, enabling the visual analysis of Ag+ in environmental water samples at low detection limits.
MICROCHEMICAL JOURNAL
(2022)
Article
Biophysics
Lijuan Gui, Jun Yan, Junyuan Zhao, Shiya Wang, Yingying Ji, Ji Liu, Jinsheng Wu, Kang Yuan, Heng Liu, Dawei Deng, Zhenwei Yuan
Summary: This study developed a novel ratiometric probe Rx-NCE to detect hypochlorite levels in foam cells and outline the boundaries of atherosclerotic plaques. The innovative application of this probe in distinguishing atherosclerotic blood vessels is also demonstrated. This research has significant value in studying ER stress and atherosclerosis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Wen-Li Jiang, Wen-Xin Wang, Guo-Jiang Mao, Ling Yan, Yan Du, Yongfei Li, Chun-Yan Li
Summary: A ratiometric near-infrared fluorescent probe CP-CO based on a coumarin-benzopyran fluorophore for imaging CO was developed with high sensitivity and enhancement ratio. The probe was successfully applied for ratiometric monitoring of cellular endogenous and exogenous CO levels, as well as fluorescence imaging in zebrafish using two-photon excitation.Moreover, CP-CO visualized the upregulation of CO under lipopolysaccharide-induced oxidative stress in a zebrafish model, showcasing its excellent ability in elucidating CO function in related biological events.
ANALYTICAL CHEMISTRY
(2021)
Article
Engineering, Environmental
Hui Liu, Haoyuan Li, Siyuan Xia, Shanshan Yu, Yujie Duan, Lei Wang, Hongxiang Zhu, Hui He
Summary: A cellulose nanocrystal-based upconversion ratiometric fluorescent nanoprobe was developed for pH monitoring and imaging by chemically bonding upconversion nanoparticles, fluorescein isothiocyanate, and rhodamine B acceptors using cellulose nanocrystals as a bridge. The probe addressed issues such as aggregation-caused quenching of dye, long donor-to-acceptor distance, and poor water dispersibility. It exhibited excellent selectivity, sensitivity, and visualization for intracellular pH levels of 5.0-7.4 with 980 nm excitation. The biocompatible probe inspired by chameleons enhanced penetration depth and showed promising results for pH monitoring and imaging in living cells and in vivo.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Analytical
Tonghui Huang, Shirong Yan, Yongbo Yu, Yunsheng Xue, Yanyan Yu, Cuiping Han
Summary: In this study, a dual-responsive ratiometric fluorescent probe PTZ-H was designed and synthesized for the separate and simultaneous detection of hypochlorite and peroxynitrite. The probe exhibited high sensitivity, specificity, and biocompatibility, allowing for targeted imaging of the two species in living cells, zebrafish, and tissues.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Jisoo Kang, Jong Min An, Eun Woo Seo, Jaehui Lee, Youngbuhm Huh, Chan Park, Jaehoon Kim, Dokyoung Kim
Summary: In this study, a new fluorescent probe for hydrazine detection was developed. The probe showed high stability and could detect hydrazine quickly and accurately.
Article
Chemistry, Applied
Wenyi Liao, Shuwei Zhang, Xuewen Wang, Yuyuan Sun, Ting Wang, Huaqian Xu, Yu Yuan, Gang Chen, Xiaodong Jia
Summary: The abnormality of Cys concentration in organism could lead to a series of diseases, so its effective detection is of great importance in disease diagnosis. Here we developed a novel ESIPT fluorescent probe HBT-IDAc based on the classic ESIPT fluorophore 2-(2'-hydroxyphenyl)benzothiazole (HBT) and indanone, which could selectively and sensitively detect Cys with a large Stokes shift up to 210 nm and the detection limit to 0.89 μM. In addition, the probe exhibited good anti-interference and low cytotoxicity, and has been successfully applied to monitor Cys in cells.
Article
Chemistry, Analytical
Feicheng Bai, Wei Du, Xia Liu, Lichao Su, Zhi Li, Tao Chen, Xiaoguang Ge, Qingqing Li, Huanghao Yang, Jibin Song
Summary: Researchers have developed a NO-responsive fluorescent nanoprobe for quantitative detection and monitoring of drug-induced liver injury. This nanoprobe can selectively detect NO in vivo, monitor DILI, and screen antidotes for acetaminophen, showing promise for early diagnosis and visualization of liver injury caused by drugs.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Ya-Nan Wei, Bo Lin, Yang Shu, Jian-Hua Wang
Summary: A novel fluorescent probe ANET was designed to target mitochondria for detecting Cys, showing advantages of ratiometric fluorescence, high selectivity, and a large Stokes shift. Imaging of Cys was confirmed in HepG2 cells using this probe.
Article
Microbiology
Farhana Mosaddeque, Shusaku Mizukami, Mohamed Gomaa Kamel, Awet Alem Teklemichael, Truong Van Dat, Satoshi Mizuta, Dinh Van Toan, Ali Mahmoud Ahmed, Nguyen Lam Vuong, Mohamed Tamer Elhady, Hoang Thi Nam Giang, Tran Ngoc Dang, Michiko Fukuda, Lam K. Huynh, Yoshimasa Tanaka, Timothy J. Egan, Osamu Kaneko, Nguyen Tien Huy, Kenji Hirayama
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2018)
Article
Chemistry, Medicinal
Satoshi Mizuta, Juliann Nzembi Makau, Ayako Kitagawa, Kanami Kitamura, Hiroki Otaki, Kodai Nishi, Ken Watanabe
Article
Chemistry, Physical
Takeshi Ishikawa, Satoshi Mizuta, Osamu Kaneko, Kazuhide Yahata
JOURNAL OF PHYSICAL CHEMISTRY B
(2018)
Article
Chemistry, Medicinal
Satoshi Mizuta, Mohammed S. O. Tagod, Masashi Iwasaki, Yoichi Nakamura, Hiroaki Senju, Hiroshi Mukae, Craig T. Morita, Yoshimasa Tanaka
Article
Biochemical Research Methods
Mohammed S. O. Tagod, Satoshi Mizuta, Yuki Sakai, Masashi Iwasaki, Kengo Shiraishi, Hiroaki Senju, Hiroshi Mukae, Craig T. Morita, Yoshimasa Tanaka
JOURNAL OF IMMUNOLOGICAL METHODS
(2019)
Article
Chemistry, Organic
Kosuke Yamamoto, Keiko Ishimaru, Satoshi Mizuta, Daishirou Minato, Masami Kuriyama, Osamu Onomura
Article
Chemistry, Multidisciplinary
Nana Nakada-Motokawa, Taiga Miyazaki, Satoshi Mizuta, Yoshimasa Tanaka, Tatsuro Hirayama, Takahiro Takazono, Tomomi Saijo, Kazuko Yamamoto, Yoshifumi Imamura, Koichi Izumikawa, Katsunori Yanagihara, Koichi Makimura, Kohsuke Takeda, Shigeru Kohno, Hiroshi Mukae
Article
Virology
Juliann Nzembi Makau, Ken Watanabe, Hiroki Otaki, Satoshi Mizuta, Takeshi Ishikawa, Yuji O. Kamatari, Noriyuki Nishida
Article
Clinical Neurology
Daisuke Ishibashi, Takeshi Ishikawa, Satoshi Mizuta, Hiroya Tange, Takehiro Nakagaki, Tsuyoshi Hamada, Noriyuki Nishida
Article
Multidisciplinary Sciences
Makoto Sumiyoshi, Taiga Miyazaki, Juliann Nzembi Makau, Satoshi Mizuta, Yoshimasa Tanaka, Takeshi Ishikawa, Koichi Makimura, Tatsuro Hirayama, Takahiro Takazono, Tomomi Saijo, Hiroyuki Yamaguchi, Shintaro Shimamura, Kazuko Yamamoto, Yoshifumi Imamura, Noriho Sakamoto, Yasushi Obase, Koichi Izumikawa, Katsunori Yanagihara, Shigeru Kohno, Hiroshi Mukae
SCIENTIFIC REPORTS
(2020)
Article
Chemistry, Medicinal
Yuki Sakai, Satoshi Mizuta, Asuka Kumagai, Mohammed S. O. Tagod, Hiroaki Senju, Tatsufumi Nakamura, Craig T. Morita, Yoshimasa Tanaka