Article
Plant Sciences
Anza-Tshilidzi Ramabulana, Paul A. Steenkamp, Ntakadzeni E. Madala, Ian A. Dubery
Summary: Plant cell culture can be manipulated using plant growth regulators to induce metabolic changes for the production of specialized metabolites. This study investigated different combination ratios of PGRs to induce metabolic changes in Bidens pilosa callus, revealing distinct metabolomic profiles dominated by chlorogenic acids. While organogenesis was not achieved, the results demonstrate the potential of using exogenous PGRs to manipulate plant metabolism for in vitro production of specialized metabolites.
Article
Environmental Sciences
Xiong Li, Liyan Tian, Boqun Li, Huafang Chen, Gaojuan Zhao, Xiangshi Qin, Yuanyuan Liu, Yongping Yang, Jianchu Xu
Summary: The green soil chelator polyaspartic acid (PASP) can enhance heavy metal phytoextraction efficiency. PASP addition improves plant growth and promotes cadmium (Cd) uptake in Bidens pilosa by improving soil-available nutrients and activating plant growth-promoting rhizobacteria. It also enhances rhizosphere-available Cd and activates rhizobacteria involved in immobilizing/mobilizing Cd, leading to increased Cd uptake by plants.
Article
Food Science & Technology
Yu-Chen Liao, Taejo Kim, Juan L. Silva, Wu-Yueh Hu, Bang-Yuan Chen
Summary: This study investigated the antioxidant activity and phenolic composition changes in coffee beans from different geographic origins and roasting degrees. The results showed that roasting degree had minimal impact on antioxidant activity and total phenolic content, but affected the levels of different phenolic compounds. Roasting could enhance the formation of purpurogallin and other phenolic compounds, compensating for the decrease in antioxidant activity due to the breakdown of chlorogenic acid.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Agricultural Engineering
Sara Alvarez-Rodriguez, Eleonora Spinozzi, Adela M. Sanchez-Moreiras, David Lopez-Gonzalez, Marta Ferrati, Giorgio Lucchini, Filippo Maggi, Riccardo Petrelli, Fabrizio Araniti
Summary: In this study, the phytotoxic potential of essential oil (EO) extracted from Carlina acaulis L. roots was tested against the weed Bidens pilosa L. The EO showed strong toxic effects on the plant metabolism, causing leaf necrosis, reduction in water content and leaf area, and an increase in dry weight/fresh weight ratio. It also damaged the photosynthetic machinery and altered the concentration of certain metabolites, indicating a reduction in photosynthetic efficiency. These findings suggest that C. acaulis EO has potential as a natural herbicide against weeds and requires further investigation.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Plant Sciences
Khuliso Mathatha, Adivhaho Khwathisi, Anza-Tshilidzi Ramabulana, Imah Mwaba, Lutendo Michael Mathomu, Ntakadzeni Edwin Madala
Summary: Bidens pilosa is an edible plant with highly sought-after nutraceutical properties. This study reports the putative identification of genes related to the accumulation of metabolites in B. pilosa, providing a basis for further research on its nutraceutical values.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Biotechnology & Applied Microbiology
Danchun Zhang, Jiajun Tu, Xiaoxia Ding, Wan Guan, Lu Gong, Xiaohui Qiu, Zhihai Huang, He Su
Summary: The chloroplast genomes of three endemic Chinese Bidens plants were sequenced, assembled, and annotated in this study. The results revealed a typical quadripartite structure and gene loss, and also suggested positive and purifying selection for certain chloroplast genes in Bidens. Phylogenetic analysis showed that these Bidens plants clustered together and formed a monophyletic clade with other Bidens species, indicating radiation adaptive selection. Additionally, inter-genic regions were identified as potential molecular markers for Bidens plant identification.
Article
Agricultural Engineering
Bai-Lin Li, Jin-Dan Xie, Fu-Da Xie, Hai-Yan Li, Juan-Juan Hu, Qian-Ran Li, Hui-Jun Liang, Zhuo-Yi Ao, Yu-Wen Fan, Wei-Jie Zhang, Jie Yuan, Jie-Wei Wu
Summary: This study found that four polyacetylenes isolated from Bidens pilosa exhibited potent insecticidal activities against various pests, with compound 1 showing activity comparable to the commercial pesticide pyrethrins. These compounds also displayed inhibitory effects on GSTs and AChE in Plutella xylostella, indicating their potential as skin-safe insecticidal agents.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Food Science & Technology
Agnieszka Micek, Justyna Godos, Achille Cernigliaro, Raffaele Ivan Cincione, Silvio Buscemi, Massimo Libra, Fabio Galvano, Giuseppe Grosso
Summary: The intake of polyphenol-rich beverages is associated with metabolic status, with individuals consuming more of these beverages less likely to have hypertension, type 2 diabetes, and dyslipidemia, particularly with a significant association with hypertension. Current scientific evidence suggests that such beverages may have a positive impact on cardio-metabolic risk factors.
Article
Environmental Sciences
Huiping Dai, Shuhe Wei, Lidia Skuza, Qing Zhang
Summary: This study compared the accumulative properties of two ecotype hyperaccumulators of Bidens pilosa L. from different climate zones, revealing significant variations in Cd concentration and biomass between the ecotypes. The differences in Cd accumulation among ecotypes may be useful for identifying genes relevant to plant hyperaccumulation.
Article
Plant Sciences
Ndamulelo Nengovhela, Dakalo T. Mbedzi, Ashwell R. Ndhlala, Lutendo M. Mathomu, Msizi I. Mhlongo, Ntakadzeni E. Madala
Summary: A comparative study was conducted on the leaves of Coccinia grandis and Coccinia rehmannii using metabolite profiling, which revealed differences in the conjugation of hydroxycinnamic acids (HCAs). The findings suggest that conjugation and isomerization processes can serve as chemotaxonomic markers and provide insights for future research on the pharmacological potency of these plants.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Environmental Sciences
Shuting Tang, Yue Xu, Kaiyue Zeng, Xin Liang, Xinwei Shi, Kehui Liu, Jiangming Ma, Fangming Yu, Yi Li
Summary: A field study compared the effects of irrigation and spraying on the phytoremediation of cadmium-contaminated soil by Bidens pilosa L. The results showed that FM-1 inoculation improved the rhizosphere soil environment and increased the extraction of Cd from the soil. Iron (Fe) and phosphorous (P) played vital roles in promoting plant growth when FM-1 was inoculated by irrigation, while Fe in leaves and stems played a vital role when FM-1 was inoculated by spraying. FM-1 inoculation also affected soil pH, dehydrogenase, oxalic acid, and promoted Cd uptake by Bidens pilosa L.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Agricultural Engineering
Micaela Chacon, Ellen Percival, Timothy D. H. Bugg, Neil Dixon
Summary: This study developed synthetic co-cultivation technology for the consolidated hydrolysis and valorization of corn cob hydroxycinnamic acids. Multiple aromatic products were isolated in high purity using cellulolytic pre-treatment, extractive fermentation, and in situ product recovery methods.
BIORESOURCE TECHNOLOGY
(2024)
Article
Engineering, Chemical
Paola Angelini, Florentina Matei, Giancarlo Angeles Flores, Roberto Maria Pellegrino, Lydie Vuguziga, Roberto Venanzoni, Bruno Tirillini, Carla Emiliani, Giustino Orlando, Luigi Menghini, Claudio Ferrante
Summary: The study confirmed the effectiveness of B. pilosa as a phytotherapy remedy against infectious disease. Higher concentrations of oligosaccharides, disaccharides, and fatty acids were found in the roots compared to other plant parts, while monoglycerides were more abundant in the stems. Peptides and diterpenoids were prominent in the leaves and roots, respectively.
Review
Pharmacology & Pharmacy
Xandy Melissa Rodriguez-Mesa, Leonardo Andres Contreras Bolanos, Antonio Mejia, Luis Miguel Pombo, Geison Modesti Costa, Sandra Paola Santander Gonzalez
Summary: Bidens pilosa L. has been traditionally used for treating immune response disorders, and its medicinal properties are attributed to its chemical components. This review focuses on the pre-clinical scientific evidence of the immunomodulatory properties of B. pilosa. The results suggest that compounds or extracts of Bidens can modulate immune cells through the presence of phenolic compounds and flavonoids. The potential use of B. pilosa as an immune response modulator for various diseases is supported, but more specialized clinical trials are needed to confirm its effectiveness.
Article
Pharmacology & Pharmacy
Simin Emamzadeh Yazdi, Heino Martin Heyman, Gerhard Prinsloo, Thomas Klimkait, Jacobus Johannes Marion Meyer
Summary: This study identified biomarkers for anti-HIV activity in Helichrysum species using metabolomics, and revealed the differences in chemical composition associated with anti-HIV activity.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Efficient N. Ncube, Kgama Mathiba, Lucia H. Steenkamp, Ian A. Dubery
Summary: In this study, the filamentous yeast Hyphozyma roseoniger was used for biocatalytic conversion of sclareol to ambradiol, a key intermediate in the perfume industry. Metabolite profiling revealed the biochemical steps involved in this conversion process, showing a decrease in sclareol concentration and an increase in ambradiol concentration over time. Although unidentified peaks were observed in gas chromatography profiles, no new intermediates were discovered in the sclareol to ambradiol conversion pathway.
BIOCATALYSIS AND BIOTRANSFORMATION
(2022)
Article
Plant Sciences
Dylan R. Zeiss, Paul A. Steenkamp, Lizelle A. Piater, Ian A. Dubery
Summary: The study discovered that Ralstonia solanacearum-derived cold shock protein csp22 can elicit an immune response in tomato plants and investigated its effects on the plant's metabolome. The results show that csp22 pre-treatment can enhance tomato's resistance against R. solanacearum infection, contributing to the understanding of plant immune system operation at an integrative level.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Physical
Efficient N. Ncube, Paul A. Steenkamp, Chris W. van der Westhuyzen, Lucia H. Steenkamp, Ian A. Dubery
Summary: This study investigates the biocatalytic conversion of sclareol to ambradiol using whole-cell biotransformation by the yeast Hyphozyma roseoniger. Metabolomics tools, including NMR spectroscopy and LC-MS, were used to identify and quantify the intermediates involved in this bioconversion. The study highlights the transformative capabilities of H. roseoniger as a biocatalyst and its potential utility in the fragrance industry.
Article
Biology
Manamele D. Mashabela, Lizelle A. Piater, Ian A. Dubery, Fidele Tugizimana, Msizi Mhlongo
Summary: Plant growth-promoting rhizobacteria (PGPR) are beneficial bacteria that promote plant growth and protect against environmental stresses through chemical communication in the rhizosphere. However, there is still limited knowledge about these interactions. Metabolomics can help uncover the complex chemical communications and their effects on plant metabolism.
Article
Multidisciplinary Sciences
Motseoa Lephatsi, Lerato Nephali, Vanessa Meyer, Lizelle A. Piater, Nombuso Buthelezi, Ian A. Dubery, Hugo Opperman, Margaretha Brand, Johan Huyser, Fidele Tugizimana
Summary: A study revealed that a microbial-based biostimulant can promote maize growth and enhance drought tolerance by inducing metabolic reconfigurations. Morphophysiological measurements and targeted metabolomics approach uncovered the impact of the biostimulant on maize plants under different water conditions (well-watered, mild and severe drought), which was further supported by gene expression and DNA methylation profiles.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Benedict C. Offor, Msizi Mhlongo, Ian A. Dubery, Lizelle A. Piater
Summary: This study investigates the receptors and co-receptor complexes involved in the perception of lipopolysaccharides (LPS) and triggering defense responses in Arabidopsis. Through protein identification and data analysis, LBP and LBR2 were found to recognize LPS and activate defense responses, while BAK1 acts as a co-receptor. The study also suggests the involvement of multiple receptors and co-receptors in LPS perception and signaling in Arabidopsis defense responses.
Article
Microbiology
Manamele D. Mashabela, Fidele Tugizimana, Paul A. Steenkamp, Lizelle A. Piater, Ian A. Dubery, Msizi I. Mhlongo
Summary: This study examines the effects of seed bio-priming with Paenibacillus and Bacillus subtilis strains on the rhizosphere metabolome of wheat cultivars and on the metabolism of above-ground tissues. The analysis reveals differential accumulation of various metabolites in the rhizosphere soil, and metabolic perturbations in the leaf tissue of bio-primed plants. These findings shed light on the biochemical nature of plant-microbe interactions and provide insights for potential metabolome engineering strategies to improve plant growth and defense.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Physical
Efficient N. Ncube, Lungile Sitole, Paul A. Steenkamp, Lucia H. Steenkamp, Ian A. Dubery
Summary: In this study, metabolomics was used to investigate the basic metabolic capabilities of H. roseonigra. The results showed that the identified discriminatory ions fell predominantly in the primary metabolism class, and no terpene synthesis was flagged. This study provides novel insights into the biochemistry of H. roseonigra and has important implications for further research.
Article
Biochemistry & Molecular Biology
Msizi Mhlongo, Lizelle A. Piater, Ian A. Dubery
Summary: The rhizosphere microbiome plays a crucial role in plant health, and the volatile organic compounds (VOCs) secreted by plant-beneficial rhizobacteria (PGPR) have potential as eco-friendly substitutes for agrochemicals. In this study, the VOCs secreted by four PGPR strains were analyzed, revealing species-specific and strain-specific compounds that could be used as biomarkers for classification and agricultural selection.
Article
Plant Sciences
Thabiso E. Letseka, Ntjana J. Sepheka, Ian A. Dubery, Mosotho J. George
Summary: This study reports a simple, rapid, and robust technique called headspace bubble-in-drop microextraction (BID-SPME) for screening essential oils in oil-bearing plants. The method only requires a small amount of plant material and can extract at least 80% of the oil constituents. The extracted compounds can be easily annotated using gas chromatography-mass spectrometry.
Article
Biochemistry & Molecular Biology
Claude Y. Hamany Y. Djande, Paul A. A. Steenkamp, Lizelle A. A. Piater, Fidele Tugizimana, Ian A. A. Dubery
Summary: Designing innovative biological crop protection strategies using eco-friendly alternatives to conventional biocidal agrochemicals is important. This study aimed to investigate the metabolic reprogramming in barley plants after treatment with three proposed dichlorinated inducers of acquired resistance. The results showed changes in the levels of both primary and secondary metabolites, including the activation of the phenylpropanoid pathway. Furthermore, the study highlighted the role of dichlorinated small molecules as inducers of plant immunity. This report provides valuable insights for metabolomics-guided plant improvement programs.
Review
Plant Sciences
Chanel J. Pretorius, Ian A. Dubery
Summary: Avenanthramides are phytoalexins produced in oat plants in response to pathogen attack. These compounds have multifunctional properties as plant defense substances, antimicrobial agents, and antioxidants, and also exhibit medicinal and pharmaceutical uses important for human health.
Article
Plant Sciences
Nikita da Camara, Ian A. Dubery, Lizelle A. Piater
Summary: Proteins play a crucial role in regulating innate immune response in plants under biotic or abiotic stresses. Isonitrosoacetophenone (INAP), an unusual stress metabolite, can induce plant defense responses. Transcriptomic and metabolomic studies of INAP-treated plant systems have provided insight into its defense-inducing and priming capabilities. In this study, a proteomic approach was used to examine time-dependent responses to INAP in Nicotiana tabacum cell suspensions. Proteome analysis revealed changes in defense-related proteins, as well as proteins involved in biosynthesis, transport, DNA transcription, metabolism, energy, translation, signaling, and response regulation. These findings highlight the importance of proteomic changes in priming induced by INAP treatment.
Article
Plant Sciences
Babra Moyo, Nikita Tawanda Tavengwa, Ian Dubery, Ntakadzeni Edwin Madala
Summary: In this study, the chemical interactions between V. combreticola and two of its host plants were investigated using UHPLC-q-TOF-MS profiling and molecular networking. The phytochemistry of V. combreticola was found to be independent and distinct from that of the host plants, and a unique chemistry of phenolic acid esterification to quinic acid was observed.
JOURNAL OF PLANT INTERACTIONS
(2023)
Article
Biochemistry & Molecular Biology
Claude Y. Hamany Djande, Fidele Tugizimana, Paul A. Steenkamp, Lizelle A. Piater, Ian A. Dubery
Summary: (English Summary:)
This study investigates the biochemical processes involved in the response of barley plants pre-treated with 3,5-dichloroanthranilic acid (3,5-DCAA) to Pyrenophora teres f. teres (Ptt). The results show that 3,5-DCAA delays the onset and reduces the severity of symptoms caused by Ptt. Metabolomic analyses reveal potential markers of the primed and naive plant responses to Ptt, including organic acids, amino acids, and hydroxycinnamic acids.