Article
Biochemistry & Molecular Biology
Fang He, Felix Umrath, Siegmar Reinert, Dorothea Alexander
Summary: This study investigated the effects of mesenchymal stem cells from bone marrow on macrophage polarization, revealing that under 5% hPL supplementation, JPCs effectively induced macrophage polarization. The results showed improved immunomodulatory properties of JPCs under this condition, highlighting the potential for JPCs in regenerative medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Pengfei Li, Zhifang Hao, Jingyu Wu, Chen Ma, Yintai Xu, Jun Li, Rongxia Lan, Bojing Zhu, Pengyu Ren, Daidi Fan, Shisheng Sun
Summary: This study systematically investigated the protein expression profiles of M1 and M2 phenotypes in human THP-1 and mouse RAW264.7 macrophages, revealing significant divergences between the two cell lines while also identifying some common up-regulated proteins in M1 macrophages. These data serve as important resources for further studies of macrophage-associated diseases in experimental pathology using human and mouse cell line models.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Valeria Longo, Noemi Aloi, Elena Lo Presti, Antonino Fiannaca, Alessandra Longo, Giorgia Adamo, Alfonso Urso, Serena Meraviglia, Antonella Bongiovanni, Fabio Cibella, Paolo Colombo
Summary: This study investigates the effects of PBDE-47 treatment on the miRNA cargo of sEVs and their downstream effects on bystander macrophages. The sEVs purified from PBDE-47 treated cells were found to modulate the expression of 12 miRNAs in resting THP-1 cells. Furthermore, PBDE-47 treatment may impair immune activity and down-regulate differentiation markers in macrophages. These findings suggest that PBDE-47 perturbs cellular regulatory pathways and affects macrophage differentiation markers by altering miRNA cargo.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Engineering, Biomedical
Patrick Thomas Coburn, Alexandre Camille Herbay, Mattia Berrini, Nicole Y. K. Li-Jessen
Summary: This study evaluated the immunological impact of varying hydrogel stiffness on anti-inflammatory activity. Results showed that increasing hydrogel stiffness was associated with increased anti-inflammatory activity. The study highlighted the importance of considering hydrogel stiffness from an immunological viewpoint when designing novel vocal fold hydrogels.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Biochemistry & Molecular Biology
Nora Feuerer, Daniel A. Carvajal Berrio, Florian Billing, Soeren Segan, Martin Weiss, Ulrich Rothbauer, Julia Marzi, Katja Schenke-Layland
Summary: THP-1 and MDMs are widely used in vitro model systems for studying innate immunity, wound healing, and tissue regeneration, showing significant molecular changes in activated cells, emphasizing the importance of choosing the right cell type for research outcomes. Raman microspectroscopy is a sensitive tool for investigating cell-specific responses to activation stimuli and monitoring molecular changes in subcellular structures.
Article
Nanoscience & Nanotechnology
Siyu Wu, Jiayi Ma, Jun Liu, Chun Liu, Su Ni, Ting Dai, Yan Wang, Yiping Weng, Hongbin Zhao, Dong Zhou, Xiubo Zhao
Summary: In this study, a nanostructured scaffold loaded with telmisartan was developed to modulate the immune response and promote bone healing. The results showed that telmisartan-loaded scaffolds exhibited anti-inflammatory activity and enhanced bone regeneration by regulating macrophage polarization.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Medicine, Research & Experimental
Zihao Chang, Qiunan Zhang, Qian Hu, Yuqi Liu, Lanzhen Zhang, Runping Liu
Summary: The study shows that the total tannin fraction of Terminalia bellirica (TB-TF) can inhibit hepatocellular carcinoma growth, promote the polarization of tumor-associated macrophages towards an anti-tumor phenotype, increase T cell infiltration, and restore cytotoxic function of CD8+T cells.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Engineering, Biomedical
Sandra Camarero-Espinosa, Maria Carlos-Oliveira, Hong Liu, Joao F. Mano, Nicole Bouvy, Lorenzo Moroni
Summary: The research investigates the immunomodulatory properties of dual-porosity scaffolds with varying mechanical and topographical features, demonstrating the significant impact of the interplay between scaffold stiffness and surface properties on immune response and healing processes.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Rui Medeiros, Bruno Horta, Joana Freitas-Silva, Jani Silva, Francisca Dias, Emilia Sousa, Madalena Pinto, Fatima Cerqueira
Summary: Xanthone derivative 1 has shown strong inhibitory effects on cancer cell metabolic activity, especially on prostate cancer cells. It is also capable of modulating macrophage functions.
Article
Biochemistry & Molecular Biology
Davy Aubeux, Solene Tessier, Fabienne Perez, Valerie Geoffroy, Alexis Gaudin
Summary: This study evaluated the effect of LXA4 on macrophage activity and phenotype modulation of polarized M1 and M2 macrophages. The results showed that LXA4 did not affect the viability and phagocytic activity of macrophages, but exhibited anti-inflammatory properties by reducing pro-inflammatory cytokine expression in M1 and increasing anti-inflammatory cytokine expression in M2 macrophages.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Immunology
Kritsada Pruksaphon, Artid Amsri, Patcharin Thammasit, Joshua D. Nosanchuk, Sirida Youngchim
Summary: Pathogenic eukaryotes, such as fungi, release extracellular vesicles (EVs) that are composed of various bioactive components. These EVs contain virulence-associated molecules and play an important role in disease pathogenesis. Studies on Evans of Talaromyces marneffei show that they contain important virulence factors, stimulate macrophage activation, and enhance antimicrobial activity.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Qian-Qian Wan, Jin-Long Sun, Yu-Xuan Ma, Luke C. Noble, Yan Dong, Zhi-Hong Feng, Jun-Ting Gu, Yi-Rong Wang, Wan-Rong Wang, Brian E. Bergeron, Kai Jiao, Franklin R. Tay, Li-Na Niu
Summary: The study revealed that TSCs modulate bone regeneration by inducing macrophage polarization into the M2 phenotype, enhancing osteogenic potential of mesenchymal stromal cells, and promoting healing of bone defects.
APPLIED MATERIALS TODAY
(2021)
Review
Pharmacology & Pharmacy
Chizumi Abe, Maharshi Bhaswant, Teruo Miyazawa, Taiki Miyazawa
Summary: Cancer is characterized by the rapid growth and spread of aberrant cells, leading to metastasis. Widespread metastases are the main cause of death in cancer patients. Different types of cancer cells vary in their proliferation and response to treatment, and conventional drugs often have harmful side effects. Utilizing exosomes as drug carriers for targeted therapy and regulating macrophage polarization in the tumor microenvironment show promise as natural and harmless approaches to cancer treatment.
Article
Engineering, Biomedical
Yaping Wang, Jingrong Wang, Rui Gao, Xiang Liu, Zujian Feng, Chuangnian Zhang, Pingsheng Huang, Anjie Dong, Deling Kong, Weiwei Wang
Summary: A synthetic fibrous glycopeptide hydrogel was developed and composited with 3D-printed PCL/nHA scaffold for cranial bone regeneration, showing significant promotion of stem cell proliferation and osteogenic differentiation as well as enhanced interaction between macrophages and stem cells. The composite scaffold exhibited superior efficacy in bone regeneration and osseointegration, creating a reparative microenvironment similar to normal cranium with increased anti-inflammatory macrophages and osteoblasts.
Article
Pharmacology & Pharmacy
Yongheng Gao, Xia Huang, Hongwei Lin, Mingxuan Zhao, Wei Liu, Wangping Li, Luyao Han, Qiong Ma, Chuan Dong, Yujuan Li, Ying Hu, Faguang Jin
Summary: PM2.5 exposure leads to elevated levels of ROS in the lungs, causing oxidative stress and inflammation. Treatment with Antioxi-EVs derived from ADSCs can suppress ROS levels and reduce oxidative stress, inflammation, and lung injury induced by PM2.5 through anti-oxidative stress and immunomodulation pathways.