Article
Agronomy
Duo Wang, Ru Li, Ya-Xue Wu, Xiang-Yuan Fan, Xiao-Ya Liu, Feng Yang, Tian-Tian Zhang, Jing-Yi Ma, Yong-Hong Hu
Summary: This study reports the molecular characterization of the HK gene and its potential application in vaccines against Haemaphysalis longicornis. The results showed that protein and DNA-based vaccines could increase antibody levels in immunized animals. Vaccination trials on rabbits demonstrated that protein and DNA vaccines reduced tick infestation, egg mass weight, and egg hatching rate. Protein vaccination provided 65.6% protection against adult female ticks, while DNA vaccination provided 51.8% protection. In conclusion, the HK protein and DNA vaccines have potential as effective tick control tools and inhibiting glucose metabolism may be a new strategy for tick control.
PEST MANAGEMENT SCIENCE
(2023)
Article
Immunology
Maki Kuniyori, Nariko Sato, Naoaki Yokoyama, Shin-ichiro Kawazu, Xuenan Xuan, Hiroshi Suzuki, Kozo Fujisaki, Rika Umemiya-Shirafuji
Summary: In this study, the molecular mechanisms of transovarial transmission of the Babesia parasite in ticks were investigated. The expression profiles of key molecules involved in tick oogenesis were analyzed, revealing the impact of Babesia infection on yolk protein precursor synthesis and uptake. The accumulation of HIVg-2 was found to be associated with Babesia infection and transmission in ticks. These findings provide important insights into the transovarial transmission mechanisms of pathogens in tick vectors.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Desmond Onyeka Agwunobi, Miao Zhang, Xinyue Shi, Shiqi Zhang, Meng Zhang, Tongxuan Wang, Abolfazl Masoudi, Zhijun Yu, Jingze Liu
Summary: DNA methylation, mediated by DNA methyltransferases (Dnmts), plays an important role in affecting organism acclimatization and adaptation to environmental changes. In this study, Dnmts in two important vector ticks were cloned and identified, revealing their functions in cold response. The results showed that the expression of Dnmts decreased after cold treatment for 3 days but increased with longer treatment time, with Dnmt1 showing significant fluctuations in both tick species.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Entomology
Ningmei Wang, Aimeng Ji, Abolfazl Masoudi, Shuang Li, Yuhong Hu, Yefei Zhang, Zhijun Yu, Han Wang, Hui Wang, Jingze Liu
Summary: In this study, the protein regulation mechanism of low-temperature tolerance in Haemaphysalis longicornis was investigated. The results showed that maintaining cell homeostasis, cell viability, and enhancing cell tolerance were crucial for the ticks to survive under low temperatures. Furthermore, arylamine N-acetyltransferase, inositol polyphosphate multikinase, and dual-specificity phosphatase were identified as potential key proteins in low-temperature tolerance.
Article
Veterinary Sciences
Hongfei Shi, Bozhen Li, Jie Li, Shiwei Chen, Lulu Wang, Zhenzhen Bai, Li Zhu, Baolong Yan, Lunguang Yao
Summary: This study provides the first molecular evidence of natural Ca. M. haemobos infection in dogs and tick species H. longicornis and R. (B.) microplus from China.
BMC VETERINARY RESEARCH
(2022)
Article
Multidisciplinary Sciences
Kofi Dadzie Kwofie, Emmanuel Pacia Hernandez, Hayato Kawada, Yuki Koike, Sana Sasaki, Takahiro Inoue, Kei Jimbo, Fusako Mikami, Danielle Ladzekpo, Rika Umemiya-Shirafuji, Kayoko Yamaji, Tetsuya Tanaka, Makoto Matsubayashi, Md Abdul Alim, Samuel Kweku Dadzie, Shiroh Iwanaga, Naotoshi Tsuji, Takeshi Hatta
Summary: RNA activation (RNAa) is a growing area of research that involves the upregulation of specific genes through the targeting of promoter sequences and AU-rich elements in mRNA molecules. This study provides the first evidence of RNAa in ticks and highlights its potential as a tool for gene overexpression in future tick biology studies, with the aim of reducing the global burden of tick-borne diseases.
SCIENTIFIC REPORTS
(2023)
Article
Veterinary Sciences
Jin Luo, Wenge Liu, Qiaoyun Ren, Xiaokai Song, Ruofeng Yan, Guangyuan Liu, Xiangrui Li
Summary: Ribosomal protein L12 (RpL12) plays an important role in ovarian development and engorgement in ticks. This study provides further insights into the functional characteristics and expression levels of RpL12 in Haemaphysalis longicornis, and suggests the potential use of RpL12 for the generation of anti-tick vaccines.
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
(2022)
Article
Veterinary Sciences
Jin Luo, Wenge Liu, Qiaoyun Ren, Xiaokai Song, Ruofeng Yan, Guangyuan Liu, Xiangrui Li
Summary: RpL12 plays an important role in ovarian development and engorgement in Haemaphysalis longicornis, and could potentially be used to generate anti-tick vaccines.
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
(2022)
Article
Parasitology
Alec T. Thompson, Seth A. White, Emily E. Doub, Prisha Sharma, Kenna Frierson, Kristen Dominguez, David Shaw, Dustin Weaver, Stacey L. Vigil, Denise L. Bonilla, Mark G. Ruder, Michael J. Yabsley
Summary: This study surveyed ticks collected from animals in 23 different states in the eastern USA, with the primary goal of better characterizing the distribution and host associations of the exotic tick H. longicornis. New distribution data on tick species of veterinary or medical importance were also obtained.
PARASITES & VECTORS
(2022)
Article
Immunology
Jialing Yao, Zhengmao Xu, Zeyu Sun, Keke Zhou, Jinmiao Lu, Rongsheng Mi, Yan Huang, Xiangan Han, Keyi Ren, Zhaoguo Chen, Haiyan Gong
Summary: In this study, a ligand for LRR from Haemaphysalis longicornis (HILRR) was found to recognize BmActin in Babesia microti, and was shown to modulate innate immunity in ticks to influence Babesia growth. This discovery may contribute to the development of anti-Babesia vaccines or drugs.
INFECTION AND IMMUNITY
(2021)
Article
Entomology
Seong Yoon Kim, Choong Won Seo, Hee Il Lee
Summary: In this study, the presence of SFTSV in ticks collected near the residence of a SFTS patient was investigated. Through flagging and dry ice-baited traps, tick samples were collected from the vegetation around the patient's residence in Chuncheon City, Gangwon Province, South Korea. SFTSV was detected in 5.3% of the collected tick samples, and the genetic sequences were highly identical to the SFTSV found in the patient. This study provides the first molecular evidence of the correlation between SFTSV in questing ticks and SFTS patients in South Korea.
EXPERIMENTAL AND APPLIED ACAROLOGY
(2023)
Article
Agronomy
Matthew Bickerton, Julia Gonzalez, Andrea Egizi, Alvaro Toledo
Summary: The study evaluated the susceptibility of the Asian longhorned tick to various acaricides used in tick control. The results showed that the tick species is currently not resistant to the tested acaricides. Responsible integrated management and early detection of resistance are important for long-term efficacy of tick control products.
PEST MANAGEMENT SCIENCE
(2023)
Article
Microbiology
Paul Franck Adjou Moumouni, Souichirou Naomasa, Bumduuren Tuvshintulga, Nariko Sato, Kiyoshi Okado, Weiqing Zheng, Seung-Hun Lee, Juan Mosqueda, Hiroshi Suzuki, Xuenan Xuan, Rika Umemiya-Shirafuji
Summary: In this study, a homologue of Rhipicephalus microplus ATAQ in H. longicornis (HlATAQ) was identified, characterized, and evaluated for its immunogenic potential. HlATAQ was found to be involved in blood-feeding and oviposition processes in the tick's midgut and Malpighian tubules. Silencing of HlATAQ did not result in significant phenotypic changes.
Article
Entomology
Lei Liu, Hao Tang, De-Yong Duan, Jin-Bao Liu, Jie Wang, Li-Li Feng, Tian-Yin Cheng
Summary: The study investigated the AV422 protein in Haemaphysalis flava ticks, revealing its significantly higher expression in salivary glands and cuticles compared to ovaries, along with its anticoagulant activity. Further research on its functional properties is necessary to confirm it as a suitable antigen candidate for the development of broad-spectrum vaccines against ticks.
EXPERIMENTAL AND APPLIED ACAROLOGY
(2021)
Article
Parasitology
Lei Liu, Fen Yan, Lu Zhang, Zhi-feng Wu, De-yong Duan, Tian-yin Cheng
Summary: This study analyzed the tick/host protein components in the hemolymph of Haemaphysalis flava and identified numerous tick- and host-derived proteins. The protein profile revealed the important physiological functions of these proteins in blood coagulation, digestion of blood meal, and innate immunity. Further investigations are needed to characterize tick-derived proteins in hemolymph.
PARASITES & VECTORS
(2022)
Article
Agriculture, Dairy & Animal Science
Kalibixiati Aimulajiang, Man Cao, Shuyi Liao, Muhammad Ali-ul-Husnain Naqvi, Xiaowei Tian, Zehua Li, Mingmin Lu, Shakeel Ahmed Lakho, Xiangrui Li, Lixin Xu, Xiaokai Song, RuoFeng Yan
Article
Immunology
Muhammad Ehsan, Muhammad Haseeb, Ruisi Hu, Haider Ali, Muhammad Ali Memon, Ruofeng Yan, Lixin Xu, Xiaokai Song, Xingquan Zhu, Xiangrui Li
Article
Biochemistry & Molecular Biology
Muhammad Ali-ul-Husnain Naqvi, Muhammad Ali Memon, Tahseen Jamil, Sana Zahra Naqvi, Kalibixiati Aimulajiang, Javaid Ali Gadahi, Lixin Xu, Xiaokai Song, Xiangrui Li, Ruofeng Yan
Article
Microbiology
Yongqian Bu, Caiwen Jia, Xiaowei Tian, Kalibixiati Aimulajiang, Muhammad Ali Memon, Ruofeng Yan, Xiaokai Song, Lixin Xu, Xiangrui Li
Article
Microbiology
Muhammad Ehsan, Javaid Ali Gadahi, Muhammad Waqqas Hasan, Muhammad Haseeb, Haider Ali, Ruofeng Yan, Lixin Xu, Xiaokai Song, Xing-Quan Zhu, Xiangrui Li
Article
Immunology
Zhenchao Zhang, Yuhua Li, Haoran Li, Xiaoxiao Song, Zhongshan Ma, Haoran Lu, Shuyue Liu, Yi Zhao, Mengyao Tan, Shuai Wang, Xiangrui Li
Article
Nanoscience & Nanotechnology
Qiangqiang Wang, Xiaoke Sun, Xin Huang, Jianmei Huang, Muhammad Waqqas Hasan, RuoFeng Yan, Lixin Xu, Xiaokai Song, Xiangrui Li
Summary: The study demonstrated that using nanoparticles as carriers can significantly enhance the immune response of Haemonchus contortus HCA59 protein, especially when using rHCA59-Chitosan-PLGA nanoparticles. This research provides a new direction for the development of nanovaccines in the future.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Pharmacology & Pharmacy
Zhengqing Yu, Wenxi Ding, Muhammad Tahir Aleem, Junzhi Su, Junlong Liu, Jianxun Luo, Ruofeng Yan, Lixin Xu, Xiaokai Song, Xiangrui Li
Summary: The study demonstrated that encapsulation of T. gondii antigens in chitosan nanospheres induced Th1-biased immune responses in mice and chickens, resulting in increased production of antibodies, IFN-gamma, IL-4, and IL-17, and regulated production of IL-10. Furthermore, rTgPSA1/CS nanospheres showed significant protection against acute toxoplasmosis in both mice and chickens.
Article
Immunology
Zhengqing Yu, Yujia Lu, Zhaoyi Liu, Muhammad Tahir Aleem, Junlong Liu, Jianxun Luo, Ruofeng Yan, Lixin Xu, Xiaokai Song, Xiangrui Li
Summary: The study demonstrated that recombinant TgRPP2 (rTgRPP2) could modulate murine macrophages and enhance immune protection in mice against acute toxoplasmosis. Immunization with rTgRPP2 led to increased levels of antibodies, cytokines, and major histocompatibility complex (MHC) molecules, resulting in prolonged survival time. These results suggest that rTgRPP2 may be a potential target for drug design and vaccine development.
Article
Infectious Diseases
Hong Fei, Muhammad Ali-ul-Husnain Naqvi, Sana Zahra Naqvi, Lixin Xu, Xiaokai Song, Xiangrui Li, Ruofeng Yan
Summary: The study cloned the GILT from Trichinella spiralis and confirmed its key role in antigen processing and presentation. Knocking down the expression of Tsp-GILT using siRNA revealed that GILT may play an important role in the development of T. spiralis parasite.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Pharmacology & Pharmacy
Zhengqing Yu, Yujia Lu, Wandi Cao, Muhammad Tahir Aleem, Junlong Liu, Jianxun Luo, Ruofeng Yan, Lixin Xu, Xiaokai Song, Xiangrui Li
Summary: A novel DNA vaccine encoding T. gondii histone deacetylase SIR2 was designed, with chitosan or PLGA nanospheres to enhance efficacy, inducing a mixed Th1/Th2 immune response and reducing parasite burden in immunized animals.
Article
Immunology
Di Tian, Xiaoqian Liu, Xiangrui Li, Lixin Xu, Ruofeng Yan, Xiaokai Song
Summary: In this study, a recombinant protein and a DNA plasmid of E. maxima ROM5 were expressed and used as subunit and DNA vaccines, respectively. Vaccination with these vaccines significantly increased the proportion of T lymphocytes, cytokine levels, and antibody levels, and reduced the damage caused by the disease. The results showed that these vaccines provided moderate protection against E. maxima infection.
Article
Microbiology
Xuefang Mei, Changwei Su, Weijuan Wang, Bo Zhang, Lai Wei, Zhenchao Zhang, Xiaowei Tian, Zhenke Yang, Xiangrui Li, Aijun Duan, Shuai Wang
Summary: This study investigated the infection and genotype distribution of Blastocystis hominis (B. hominis) in two cities of Henan Province, China. The infection rates of B. hominis were similar between the two cities, but the incidence of hematochezia differed significantly from other symptoms. Five subtypes were identified, with ST3 being the most prevalent subtype.
JOURNAL OF EUKARYOTIC MICROBIOLOGY
(2023)
Article
Immunology
Muhammad Tahir Aleem, Zhaohai Wen, Zhengqing Yu, Cheng Chen, Mingmin Lu, Lixin Xu, Xiaokai Song, Xiangrui Li, Ruofeng Yan
Summary: This study investigates the inhibitory effects of different ratios of antibodies against Ts-MAPRC2 on the development of Trichinella spiralis larvae and found that rTs-MAPRC2-Ab greatly inhibited the activity of Ts-MAPRC2 and reduced larval infectivity and survival. siRNA-Ts-MAPRC2 effectively silenced the Ts-MAPRC2 gene and significantly reduced larval survival and infectivity. The results suggest that Ts-MAPRC2 may be a potential molecular target for vaccines against Trichinella spiralis infection.
Article
Veterinary Sciences
Lixin Xu, Quanjia Xiang, Mongqi Li, Xiaoting Sun, Mingmin Lu, Ruofeng Yan, Xiaokai Song, Xiangrui Li
Summary: This study evaluated the effects of Eimeria mitis on Eimeria necatrix or Eimeria tenella infection and found that the presence of E. mitis exacerbated the outcome of co-infection with E. tenella. However, it had little impact on the infection outcome of E. necatrix. These results suggest the importance of considering the relationships among different Eimeria species when using mixed infection models.
VETERINARY SCIENCES
(2022)