期刊
BIOMOLECULES
卷 10, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/biom10040512
关键词
antioxidant peptides; Salamandra salamandra; portuguese biodiversity; bioactive molecules
资金
- FCT-Fundacao para a Ciencia e a Tecnologia, I.P. [PTDC/BII_BIO/31158/2017]
- Brazilian National Council for Scientific and Technological Development (CNPq) [420449/2018-3, 428211/2018-6]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-Brasil (CAPES) [001]
- Fundação para a Ciência e a Tecnologia [PTDC/BII-BIO/31158/2017] Funding Source: FCT
Amphibian skin is a multifunctional organ that plays key roles in defense, breathing, and water balance. In this study, skin secretion samples of the fire salamander (Salamandra salamandra) were separated using RP-HPLC and de novo sequenced using MALDI-TOF MS/MS. Next, we used an in silico platform to screen antioxidant molecules in the framework of density functional theory. One of the identified peptides, salamandrin-I, [M + H](+) = 1406.6 Da, was selected for solid-phase synthesis; it showed free radical scavenging activity against DPPH and ABTS radicals. Salamandrin-I did not show antimicrobial activity against Gram-positive and -negative bacteria. In vitro assays using human microglia and red blood cells showed that salamandrin-I has no cytotoxicity up to the concentration of 100 mu M. In addition, in vivo toxicity tests on Galleria mellonella larvae resulted in no mortality at 20 and 40 mg/kg. Antioxidant peptides derived from natural sources are increasingly attracting interest. Among several applications, these peptides, such as salamandrin-I, can be used as templates in the design of novel antioxidant molecules that may contribute to devising strategies for more effective control of neurological disease.
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