Article
Medicine, Research & Experimental
Zhong-Yan Zhou, Wen-Ting Shi, Jing Zhang, Wai-Rong Zhao, Ying Xiao, Kai-Yu Zhang, Jie Ma, Jing-Yi Tang, Yu Wang
Summary: Sodium tanshinone IIA sulfonate (STS) exhibits potent endothelial protective effect against Hcy-induced cell injury, which is related to the inhibition of oxidative stress and mitochondrial dysfunction, as well as activation of AKT/MAPKs, SIRT1/NRF2/HO-1, and NNMT/MNA signaling pathways.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Ankita Sarkar, Sourav Dutta, Malinki Sur, Semanti Chakraborty, Puja Dey, Piyali Mukherjee
Summary: This study reveals the regulation of Sarm1 activation by endogenous NAD(+) levels, showing that the early loss of endogenous NAD(+) levels acts as a "biological trigger" for Sarm1 activation following rotenone treatment. Moreover, replenishing NAD(+) through PARP inhibition restores mitochondrial complex I activity and prevents Sarm1 activation in rotenone-treated cells.
Article
Nanoscience & Nanotechnology
Mingzhou Ye, Yi Zhao, Yuyuan Wang, Ruosen Xie, Yao Tong, John-Demian Sauer, Shaoqin Gong
Summary: Researchers have developed NAD-loaded nanoparticles that can effectively replenish energy, suppress inflammation, and improve multiorgan injuries, providing an innovative and translational approach to treat sepsis efficiently.
NATURE NANOTECHNOLOGY
(2022)
Article
Neurosciences
Chen Shen, Cong Chen, Tong Wang, Tong-Yao Gao, Min Zeng, Yun-Bi Lu, Wei-Ping Zhang
Summary: Nicotinamide phosphoribosyltransferase (NAMPT) is a key enzyme in the salvaging synthesis pathway of nicotinamide adenine dinucleotide (NAD). Depletion of NAMPT can lead to cognitive deficiency in hippocampus neurons and increase the risk of neurodegeneration due to mitochondrial dysfunction.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Min Li, Lydie Plecita-Hlavata, Evgenia Dobrinskikh, B. Alexandre McKeon, Aneta Gandjeva, Suzette Riddle, Aya Laux, Ram Raj Prasad, Sushil Kumar, Rubin M. Tuder, Hui Zhang, Cheng-Jun Hu, Kurt R. Stenmark
Summary: The study found that abnormal SIRT3 activity is related to mitochondrial dysfunction in adventitial fibroblasts from patients with pulmonary hypertension. Restoration of SIRT3 activity can reduce mitochondrial protein acetylation and restore mitochondrial function.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Peter Tsvetkov, Julia Adler, Romano Strobelt, Yaarit Adamovich, Gad Asher, Nina Reuven, Yosef Shaul
Summary: This passage demonstrates the interaction between SIRT1 and NQO1, indicating that SIRT1 can regulate the expression of NQO1 and exert a protective role under mitochondrial stress.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Chemistry, Analytical
Jie Zhang, Zengchen Liu, Fengshou Tian, Yahong Chen
Summary: A fluorescent compound derived from coumarin and hemicyanine was synthesized and characterized in this paper, showing higher ratiometric fluorescence response activity toward NAD(P)H compared to other commonly coexisting compounds in the cell microenvironment. The fluorescence identification mechanism was deduced to be a redox reaction between the sensor and NAD(P)H. This ratiometric fluorescent probe provides a theoretical basis for sensing NAD(P)H in vitro and in vivo, and was used to build a sensitive detection platform of NAD(P)H-dependent enzyme activity based on the fluorescence method.
ANALYTICAL METHODS
(2021)
Article
Cardiac & Cardiovascular Systems
Xin Liu, Yabing Zhang, Yan Deng, Lin Yang, Wei Ou, Maodi Xie, Lin Ding, Chunling Jiang, Hai Yu, Qian Li, Tao Li
Summary: This study identified a distinct protein acetylation pattern in the hearts of patients with HFpEF, and proposed nicotinamide riboside (NR) as a potential agent to lower acetylation levels and improve HFpEF phenotypes.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2022)
Article
Immunology
Yao Zhao, Jiawei Zhang, Yaling Zheng, Yaxuan Zhang, Xiao Jie Zhang, Hongmei Wang, Yu Du, Jian Guan, Xiuzhe Wang, Jianliang Fu
Summary: NAD(+) administration showed protective effects on cognitive impairment and neuroinflammation in chronic cerebral hypoperfusion models in vivo and in vitro, by activating the Sirt1/PGC-1 alpha pathway for mitochondrial protection and ROS inhibition.
JOURNAL OF NEUROINFLAMMATION
(2021)
Article
Pharmacology & Pharmacy
Fei Liu, Longhui Yuan, Lan Li, Jingchao Yang, Jingping Liu, Younan Chen, Jie Zhang, Yanrong Lu, Yujia Yuan, Jingqiu Cheng
Summary: Senescence of bone marrow mesenchymal stem cells (BMSCs) is a leading cause of osteoporosis. SIRT3, an essential NAD-dependent histone deacetylase, is associated with BMSC senescence-mediated bone degradation and mitochondrial/heterochromatic disturbance. S-sulfhydration of cysteine residues enhances SIRT3 activity. The underlying mechanism of SIRT3 S-sulfhydration on mitochondrial/heterochromatic homeostasis in BMSC senescence is unknown. The study demonstrates the role of SIRT3 S-sulfhydration in stabilizing heterochromatin and mitochondrial homeostasis in counteracting BMSC senescence, providing a potential target for the treatment of degenerative bone diseases.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Endocrinology & Metabolism
Keke Wu, Biao Li, Yingxu Ma, Tao Tu, Qiuzhen Lin, Jiayi Zhu, Yong Zhou, Na Liu, Qiming Liu
Summary: This study aimed to investigate the role of NMN in regulating HIF-1 alpha and fibrosis in hypoxic adipose tissue. It was found that NMN attenuated hypoxia-induced adipose fibrosis and inflammation by restoring the compromised NAD(+)/SIRT1 axis and inhibiting HIF-1 alpha activation. These findings suggest that NMN may be a potential therapeutic agent for the treatment of adipose fibrosis and inflammation.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Editorial Material
Cell Biology
Lucia Sedlackova, Tetsushi Kataura, Sovan Sarkar, Viktor I. Korolchuk
Summary: Age-related human pathologies present with a multitude of molecular and metabolic phenotypes, which contribute to tissue degeneration. Lack of understanding of the interdependence of these molecular pathologies limits therapeutic intervention strategies. In this study, we investigate the molecular events underlying cellular viability loss in autophagy deficiency associated with aging and age-related disease. We discover a novel axis linking autophagy and a metabolite, NAD, which mediates cellular demise. By elucidating the steps from mitochondrial recycling to NAD maintenance, we identify targets for therapeutic interventions in autophagy dysfunction.
Article
Biochemistry & Molecular Biology
Feng Zhang, Xinying Zhu, Ping Yu, Tingna Sheng, Yuqing Wang, Yilu Ye
Summary: Crocin ameliorates depression in mice, possibly by regulating the NAMPT-NAD+-SIRT1 pathway.
NEUROCHEMISTRY INTERNATIONAL
(2022)
Article
Cell Biology
Tibor Kristian, Arman J. Karimi, Adam Fearnow, Jaylyn Waddell, Mary C. McKenna
Summary: The knockout of SIRT3 leads to reduced activity in mitochondrial and TCA cycle metabolism in the brain, impacting both neuronal and astrocytic energy metabolism and neurotransmitter synthesis. This is likely due to hyperacetylation of mitochondrial enzymes, resulting from suppressed SIRT3 activity in the brain.
Article
Cell Biology
Joanna Ruszkiewicz, Ylea Papatheodorou, Nathalie Jaeck, Jasmin Melzig, Franziska Eble, Annika Pirker, Marius Thomann, Andreas Haberer, Simone Rothmiller, Alexander Buerkle, Aswin Mangerich
Summary: In this study, the role of NAD(+) depletion and elevation in the genotoxic stress response to sulfur mustard derivatives was investigated. The results revealed that NAD(+) is an important molecule in the pathomechanism of sulfur mustard derivatives, exhibiting compound-specificity and cell line-dependent effects.
Review
Cell & Tissue Engineering
Eun Seong Hwang, Jeong Soo Ok, SeonBeom Song
STEM CELL REVIEWS AND REPORTS
(2016)
Review
Cell & Tissue Engineering
Eun Seong Hwang, Jeong Soo Ok, SeonBeom Song
STEM CELL REVIEWS AND REPORTS
(2016)
Article
Cell Biology
Seon Beom Song, Eun Seong Hwang
Review
Endocrinology & Metabolism
Seon Beom Song, Jin Sung Park, Gu June Chung, In Hye Lee, Eun Seong Hwang
Article
Biochemistry & Molecular Biology
Seon Beom Song, Eun Seong Hwang
Article
Cell Biology
Seon Beom Song, Jin Sung Park, So Young Jang, Eun Seong Hwang
Summary: Research has shown that mitochondrial autophagy is activated through SIRT1 upon treatment with nicotinamide, leading to short mitochondrial fragments. This activation is attributed to the induction of mitochondrial fission, at least in part by the activation of dynamin-1-like protein (Drp1) and the suppression of its inhibitory phosphorylation. Additionally, the NAM-induced cycle of mitochondrial fragmentation and cytosolic Ca2+-mediated Drp1 dephosphorylation may contribute to sustained mitochondrial fragmentation.
Article
Biochemistry & Molecular Biology
Seon Beom Song, Woosung Shim, Eun Seong Hwang
Summary: This study examined the accumulation of lipofuscins in human fibroblasts under various stress conditions. The results showed that lipofuscins predominantly appear as granules that co-localize with autolysosomes in glucose-starved or replicative senescence cells, while they rarely manifest in the cytoplasm under oxidative stress induced by a doxorubicin pulse and chase. The findings suggest that autophagy activation triggers lipofuscin accumulation, and future treatments for lipofuscin-related diseases should consider autophagy activation.
MOLECULES AND CELLS
(2023)
Review
Biochemistry & Molecular Biology
Eun Seong Hwang, Seon Beom Song
Summary: Autophagy dysfunction is associated with various human diseases and conditions, with factors such as down-regulation of autophagy components and activators, oxidative damage, microinflammation, and impaired autophagy flux playing a role in its decline. Glucose deprivation and replicative senescence induce autophagy flux impairment primarily due to lysosomal acidity failure. Elevated levels of reactive oxygen species (ROS) affect lysosomal acidification, and mitochondrial alterations contribute to ROS generation and reduced lysosomal acidity. Understanding the molecular and cellular pathways of autophagy flux impairment in glucose-deprived cells sheds light on potential strategies for managing this condition, including modulating mitochondrial function for therapeutic interventions.
MOLECULES AND CELLS
(2023)
Article
Cell & Tissue Engineering
Jeong Su Ok, Seon Beom Song, Eun Seong Hwang
INTERNATIONAL JOURNAL OF STEM CELLS
(2018)
Article
Communication
Eun Seong Hwang
Article
Communication
Rae Seong Hong, Eun Seong Hwang
Review
Biochemistry & Molecular Biology
Eun Seong Hwang, Seon Beom Song
CELLULAR AND MOLECULAR LIFE SCIENCES
(2017)
Review
Communication
Jeong-Wook Seo, Hosik Chung, Tae-Sul Seo, Youngim Jung, Eun Seong Hwang, Cheol-Heui Yun, Hyungsun Kim
Article
Biochemistry & Molecular Biology
Seon Beom Song, So-Young Jang, Hyun Tae Kang, Bie Wei, Un-woo Jeoun, Gye Soon Yoon, Eun Seong Hwang
MOLECULES AND CELLS
(2017)
