Article
Medicine, Research & Experimental
Xingwang Zhao, Longlong Zhang, Juan Wang, Min Zhang, Zhiqiang Song, Bing Ni, Yi You
Summary: The study identified IFI27 as a potential key player in the pathogenesis of SLE, and highlighted the important role of immune cell infiltration in the progression of the disease. The research sheds light on new insights into the molecular mechanisms of SLE and provides potential markers for diagnosis and progression monitoring.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Yun Yu, Liang Liu, Long-Long Hu, Ling-Ling Yu, Jun-Pei Li, Jing-an Rao, Ling-Juan Zhu, Qian Liang, Rong-Wei Zhang, Hui-Hui Bao, Xiao-Shu Cheng
Summary: Systemic lupus erythematosus (SLE) is a chronic autoimmune disease that affects multiple organs. This study used bioinformatics methods to identify potential therapeutic targets for SLE, revealing tissue-specific expression differences in hematological markers and increased expression of CCL2, MMP9, and RSAD2 in SLE samples. These key genes could serve as potential therapeutic targets for treating SLE.
Review
Biochemistry & Molecular Biology
Haitao Yu, Yasuo Nagafuchi, Keishi Fujio
Summary: Systemic lupus erythematosus (SLE) is a clinically heterogeneous disease characterized by immune dysfunction. Clinical and immunological biomarkers are crucial for diagnosing, assessing, and controlling SLE, including novel biomarkers discovered through omics research.
Article
Genetics & Heredity
Zhihang Jiang, Mengting Shao, Xinzhu Dai, Zhixin Pan, Dongmei Liu
Summary: This study aimed to identify key genes associated with systemic lupus erythematosus (SLE) and develop potential diagnostic biomarkers. By analyzing gene expression data from the Gene Expression Omnibus (GEO) database, 11 differentially expressed genes (DEGs) were found to be functionally associated with the immune system. Additionally, transcription factor-diagnostic biomarker-microRNA network and drug-diagnostic biomarker network were constructed. Machine learning methods determined IFI44 as the optimal diagnostic biomarker for SLE, which was validated by quantitative real-time PCR (qRT-PCR). These findings have implications for the diagnosis and treatment of SLE patients.
FRONTIERS IN GENETICS
(2022)
Article
Immunology
Julius Lindblom, Lorenzo Beretta, Maria Orietta Borghi, Marta E. Alarcon-Riquelme, Ioannis Parodis
Summary: The study identified CCL8, CXCL13, and IL-1RA as potential serum biomarkers for activity in SLE, correlating with SLE Disease Activity Index 2000 (SLEDAI-2K) scores. Autoantibodies showed similar occurrence across organ domains in SLE patients, with weak correlations to activity in different organ domains.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Shingo Nakayamada, Yoshiya Tanaka
Summary: The treatment of rheumatoid arthritis was revolutionized with the use of molecular-targeted drugs that target immunoregulatory molecules. The success of treatment with these drugs prompted the development of molecular-targeted drugs for systemic lupus erythematosus. However, systemic lupus erythematosus is a disease with high heterogeneous immune abnormalities, and diverse cells or molecules can be treatment targets. Thus, the identification of subpopulations based on immune abnormalities is essential for the development of effective treatment.
Article
Genetics & Heredity
Haihong Zhang, Yanli Wang, Jinghui Feng, Shuya Wang, Yan Wang, Weisi Kong, Zhiyi Zhang
Summary: Systemic lupus erythematosus (SLE) is a complex autoimmune disease with high morbidity and mortality rates due to challenges in diagnosis. An integrated analysis of two RNA-seq datasets identified 790 common differentially expressed genes (DEGs) that are significantly enriched in immune-related processes and pathways. This analysis provides new insights into the pathogenic mechanisms and potential biomarkers for SLE.
FRONTIERS IN GENETICS
(2021)
Review
Biochemistry & Molecular Biology
James Greenan-Barrett, Georgia Doolan, Devina Shah, Simrun Virdee, George A. Robinson, Varvara Choida, Nataliya Gak, Nina de Gruijter, Elizabeth Rosser, Muthana Al-Obaidi, Maria Leandro, Michael S. Zandi, Ruth J. Pepper, Alan Salama, Elizabeth C. Jury, Coziana Ciurtin
Summary: Juvenile systemic lupus erythematosus (JSLE) is a form of lupus that occurs before 18 years of age and is associated with more severe disease phenotype, increased morbidity, and mortality compared to adult-onset SLE. Identifying biomarkers specific to JSLE clinical phenotype can lead to better patient management and outcomes. Research into various traditional and novel biomarkers is important for predicting organ involvement and improving treatment strategies for JSLE.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Lu Xiao, Feng Zhan, Shudian Lin
Summary: This study identified biomarkers and mechanisms associated with systemic lupus erythematosus (SLE) at a transcriptome level, demonstrating their clinical value in SLE patients.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Ji-Won Kim, Ju-Yang Jung, Sang-Won Lee, Wook-Young Baek, Hyoun-Ah Kim, Chang-Hee Suh
Summary: This study demonstrates that the expression of S100A8 in serum, urine, and saliva is significantly higher in patients with SLE than in healthy controls and is correlated with disease activity markers. Therefore, S100A8 protein could be a potential biomarker for SLE.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Ping Yang, Rui Xu, Fei Chen, Shanshan Chen, Adeel Khan, Liang Li, Xiaoshan Zhang, Yanbo Wang, Zhipeng Xu, Han Shen
Summary: This study investigates the gut fungal dysbiosis and ecology in patients with SLE and demonstrates the applicability of fungal species as diagnostic tools for SLE. It suggests that the interaction between the gut fungal mycobiome and the host may contribute to the pathogenesis of the disease.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Immunology
Mengmeng Xiang, Yilun Wang, Zhanyan Gao, Jie Wang, Qian Chen, Zhan Sun, Jun Liang, Jinhua Xu
Summary: This study used Mendelian randomization to assess the causal correlations between 41 inflammatory cytokines and systemic lupus erythematosus (SLE). The results suggested that CTACK and IL-17 may be associated with the risk of SLE, while several other inflammatory cytokines may be consequences of SLE development.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Yue Xin, Zhenghao He, Yang Mei, Xi Li, Zhidan Zhao, Mingming Zhao, Ming Yang, Haijing Wu
Summary: In this study, higher expression levels of RSAD2 were observed in CD4(+) T-cell subsets from the peripheral blood of SLE patients, suggesting its regulatory role in the pathogenesis of SLE. The expression of RSAD2 in CD4(+) T cells may be regulated by IFN-alpha and significantly affects the differentiation of Th17 and Tfh cells.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Immunology
Yamei Zhang, Lingling Gan, Jie Tang, Dan Liu, Gang Chen, Bei Xu
Summary: This study utilized a metabolomics approach to identify five serum metabolites that have the potential to serve as indicators for the differential diagnosis of SLE and LN. Glycerolphospholipid metabolism may play a crucial role in the development of SLE into LN.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Wei Sun, Pengchong Li, Min Wang, Yue Xu, Dan Shen, Xuan Zhang, Yudong Liu
Summary: This study identified key PANoptosis-related genes (PRGs) involved in immune dysregulation in SLE. These genes were associated with memory B cells, neutrophils, and CD8+ T cells, and enriched in interferon and IL-6-JAK-STAT3 signaling pathways. The expression levels of these genes were validated in peripheral blood mononuclear cells of SLE patients. These findings suggest that PANoptosis may be implicated in the immune dysregulation of SLE by regulating interferons and JAK-STAT signaling pathways in memory B cells, neutrophils, and CD8+ T cells.
CLINICAL IMMUNOLOGY
(2023)
Article
Medical Laboratory Technology
Heba Almaghrbi, Roberta Giordo, Gianfranco Pintus, Hatem Zayed
Summary: Non-coding RNAs (ncRNAs) are RNA molecules that have tissue-specific expression and lack protein-coding potential. Aberrant expression of ncRNAs is critical in disease onset and development, making them promising biomarkers for prognosis and diagnosis. Compared to standard biomarkers, ncRNAs, such as miRNAs and lncRNAs, provide better sensitivity and specificity for diagnosing acute myocardial infarction (AMI), leading to earlier treatment and better prognosis for patients. This review summarizes and discusses the potential clinical use of circulating ncRNAs as MI biomarkers, highlighting their specificity and sensitivity, limitations, and possible solutions.
CLINICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Fatemeh Rahimi Gharemirshamloo, Ranabir Majumder, S. Udhaya Kumar, C. George Priya Doss, Kourosh Bamdad, Fateme Frootan, Cemal Un
Summary: The human prion protein gene (PRNP) is located on chromosome 20 and mutations in this gene are associated with prion disease. The G127V mutation increases protein stability, while the E200K mutation, which has the highest mutation rate, causes Creutzfeldt-Jakob disease (CJD) and induces protein aggregation. This study aimed to identify the structural mechanisms of these mutations. Bioinformatic algorithms were used to predict the association of the E200K mutation with prion disease. Molecular dynamics simulations were performed to confirm the stability of the protein structures. The results showed that the E200K mutation led to increased fluctuations in the protein structure, while the G127V mutation stabilized the protein structure.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Hephzibah Cathryn R, Udhaya Kumar S, R. Gnanasambandan, P. Jeyaprakash Naayanan, P. Sathiyarajeswaren, M. S. Shree Devi, Satish Srinivas K, George Priya Doss C
Summary: This study investigated the cfDNA of MBC patients and identified mutations in PIK3CA and TP53 genes. The impact of these mutations on protein structure and function was studied using molecular docking and dynamics simulation.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Leepica Kapoor, S. Udhaya Kumar, Sourav De, Sujithra Vijayakumar, Nitin Kapoor, S. K. Ashok Kumar, C. George Priya Doss, Siva Ramamoorthy
Summary: This study evaluated the potential of bixin as an anti-skin aging agent and investigated its inhibitory mechanism on skin aging-related enzymes. Through a series of experiments, it was found that bixin possesses antioxidant activity and inhibitory effects on enzyme activity, while also having the ability to combat UVA-induced photoaging. Furthermore, the findings of this study lay a foundation for exploring the pharmaceutical applications of bixin in other biochemical pathways.
Review
Biochemistry & Molecular Biology
Anirban Goutam Mukherjee, Uddesh Ramesh Wanjari, Sandra Kannampuzha, Reshma Murali, Arunraj Namachivayam, Raja Ganesan, Abhijit Dey, Achsha Babu, Kaviyarasi Renu, Balachandar Vellingiri, Gnanasambandan Ramanathan, C. George Priya Doss, Nehal Elsherbiny, Amira M. Elsherbini, Alsamman M. Alsamman, Hatem Zayed, Abilash Valsala Gopalakrishnan
Summary: Polycystic ovarian syndrome (PCOS) is a complex endocrine and metabolic condition that affects women's reproductive health. The causes of PCOS are still unknown, but alterations in gut microbiota composition and disruptions in secondary bile acid production appear to be involved. PCOS is closely related to both enteric and vaginal bacteria, and patients with PCOS have changed microbiome compositions and decreased microbial diversity. The gut microbiota can interact with the environment to improve metabolic and immunological function, but dysbiosis in the gut microbiome may contribute to inflammation and insulin resistance in PCOS.
Article
Endocrinology & Metabolism
Sudharsana Sundarrajan, Arthi Venkatesan, S. Udhaya Kumar, Mohanraj Gopikrishnan, Iftikhar Aslam Tayubi, M. Aditya, Gowrishankar Bychapur Siddaiah, C. George Priya Doss, Hatem Zayed
Summary: Alzheimer's disease is a leading cause of dementia in elderly patients, with a duration of 3 to 11 years after diagnosis. The incidence of AD is increasing significantly in East Asian countries. The disease involves memory loss, behavioral issues, and cognitive impairment. The study investigates the whole exome sequences of LOAD patients of Korean origin to identify rare genetic variants that may contribute to the disorder.
METABOLIC BRAIN DISEASE
(2023)
Article
Multidisciplinary Sciences
Gheyath K. Nasrallah, Raniya Al-Buainain, Nadin Younes, Soha R. Dargham, Duaa W. Al-Sadeq, Mohamed Elhassan, Ibrahim Al-Shaar, Hadi M. Yassine, Laith J. Abu-Raddad, Mohamed M. Emara, Ahmed Ismail
Summary: HIV and Syphilis are common STIs that pose a concern and burden on healthcare systems. This study evaluated the diagnostic efficiency of the protocols used by the Medical Commission Department (MC) in Qatar for screening these infections.
Article
Genetics & Heredity
Karthik Sekaran, Rinku Polachirakkal Varghese, Mohanraj Gopikrishnan, Alsamman M. Alsamman, Achraf El Allali, Hatem Zayed, C. George Priya Doss
Summary: Microbial dysbiosis is linked to the development of diseases, including cervical cancer, which necessitates the study of vaginal microbiome. This study characterizes the microbial pathogenesis involved in cervical cancer and identifies specific microbial species associated with disease progression. Analysis of diversity, richness, and dominance reveals differences between cervical cancer and control samples, while Linear discriminant analysis Effect Size (LEfSe) predicts the association of certain microbial species and genera with cervical cancer. The results also provide functional enrichment evidence of microbial disease associations. The dataset is trained and validated using a random forest algorithm, and SHapley Additive exPlanations (SHAP) method is employed to analyze the predicted results, highlighting the potential significance of Ralstonia in predicting cervical cancer. The study confirms the presence of pathogenic microbiomes and their relationship with microbial imbalance in cervical cancer vaginal samples.
Article
Genetics & Heredity
Dalal Al-Sharshani, Dinesh Velayutham, Muthanna Samara, Reham Gazal, Ayman Al Haj Zen, Mohamed A. Ismail, Mahmoud Ahmed, Gheyath Nasrallah, Salma Younes, Nasser Rizk, Sara Hammuda, M. Walid Qoronfleh, Thomas Farrell, Hatem Zayed, Palli Valapila Abdulrouf, Manar AlDweik, John Paul Ben Silang, Alaa Rahhal, Rana Al-Jurf, Ahmed Mahfouz, Amar Salam, Hilal Al Rifai, Nader I. Al-Dewik
Summary: This study aimed to investigate the association between selected single nucleotide polymorphisms (SNPs) and dyslipidemia, as well as the increased susceptibility risks of cardiovascular diseases, type 2 diabetes mellitus, and non-alcoholic fatty liver disease. The results showed significant differences in genotypic frequencies of six SNPs between dyslipidemia patients and control individuals. These differences were found to be associated with gender, obesity, hypertension, and diabetes.
MOLECULAR GENETICS & GENOMIC MEDICINE
(2023)
Article
Medicine, General & Internal
Hrituraj Dey, Karthick Vasudevan, C. George Priya Doss, S. Udhaya Kumar, Achraf El Allali, Alsamman M. Alsamman, Hatem Zayed
Summary: By analyzing gene-gene interaction networks, this study aimed to identify the hub genes involved in Osteosarcoma and understand their interaction and impact on various biological processes and signaling pathways. The research revealed several key genes such as TP53, CCND1, CDK4, STAT3, and VEGFA, which are associated with major signaling pathways in Osteosarcoma and have the potential to be targeted for drug development.
FRONTIERS IN MEDICINE
(2023)
Article
Cell Biology
Zainularifeen Abduljaleel, Sami Melebari, Mohammad Athar, Saied Dehlawi, S. Udhaya Kumar, Syed A. Aziz, Anas Ibrahim Dannoun, Shaheer M. Malik, Jasheela Thasleem, George Priya Doss
Summary: This study examined the effectiveness of existing vaccines against hospitalization and infection caused by the Omicron variant of COVID-19, specifically for individuals who received two doses of Moderna or Pfizer vaccines and one dose of Johnson & Johnson vaccine or who were vaccinated more than five months ago. The presence of 36 variants in Omicron's spike protein, which is targeted by all three vaccines, has reduced the efficacy of antibodies in neutralizing the virus. Genetic sequencing of the SARS-CoV-2 viral sequence identified clinically significant variants, including E484K, that may pose a risk of infection despite successful immunization.
CELLULAR SIGNALLING
(2023)
Article
Multidisciplinary Sciences
Karthik Sekaran, Rinku Polachirakkal Varghese, George Priya C. Doss, Alsamman M. Alsamman, Hatem Zayed, Achraf El Allali
Summary: New evidence reveals the role of host-associated microbiomes in respiratory diseases and how their dysbiosis affects lung behavior and respiratory system functioning. Various exogenous and environmental factors impact the development of asthma and chronic lung disease. The relationship between asthma and microbes is better understood but still requires further investigation. The study analyzes sequencing data from the saliva and nasopharyngeal regions of asthma and non-asthma patients infected with SARS-CoV-2, focusing on microbial pathogenesis, exacerbation of respiratory disease, and potential diagnostic biomarkers. Analysis of the data identifies specific microbial taxa enriched in different groups and demonstrates the efficacy of a random forest algorithm for classification and identification of subgroups.
Article
Biochemistry & Molecular Biology
Nadin Younes, Mahmoud M. Al Ghwairi, Sahar Isa Da'as, Eiman Al Zaabi, Amin F. Majdalawieh, Nader Al-Dewik, Gheyath K. Nasrallah
Summary: The FinecareTM HbA1c Rapid Test is a reliable and rapid assay that can be easily implemented for long-term monitoring of HbA1c in diabetic patients, especially in small laboratory settings.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2023)