Article
Genetics & Heredity
Carli B. Jones, James R. White, Sarah E. Ernst, Karen S. Sfanos, Lauren B. Peiffer
Summary: Short read 16S rRNA amplicon sequencing is commonly used in microbiome research, but estimation inaccuracies in bacterial community composition can occur due to amplification bias. This study presents an analysis pipeline that integrates data from multiple hypervariable regions and is compatible with the Ion Torrent platform. The analysis pipeline was used to assess taxonomic classification of six amplicons from separate hypervariable regions. Different amplicons showed different specificities for taxonomic classification, which has implications for global level analyses. Generalized linear modeling was applied to statistically integrate the results from multiple hypervariable regions, enhancing the statistical evaluation of differences in community structure.
FRONTIERS IN GENETICS
(2022)
Article
Endocrinology & Metabolism
Qing Wang, Qi Wang, Lanbo Zhao, Yadi Bin, Li Wang, Lei Wang, Kailu Zhang, Qiling Li
Summary: The blood microbiome of PCOS patients showed significantly lower alpha diversity, different beta diversity, and significant taxonomic variations compared to healthy controls.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Microbiology
Jason A. Rothman, Jenna L. Riis, Katrina R. Hamilton, Clancy Blair, Douglas A. Granger, Katrine L. Whiteson
Summary: Human oral microbial communities have significant implications for oral and systemic health due to their diversity. Understanding the differences between healthy and dysbiotic oral microbiomes is important, especially within and between families. This study investigates the changes in oral microbiome composition within individuals and the impact of factors such as environmental tobacco smoke exposure, metabolic regulation, inflammation, and antioxidant potential. The findings reveal differences in oral microbiomes between children and caregivers, with shared diversity but distinct differences, and highlight the associations between the oral microbiome and various salivary markers.
Article
Biochemistry & Molecular Biology
Casey T. Finnicum, Zahraa Rahal, Maya Hassane, Warapen Treekitkarnmongkol, Ansam Sinjab, Rhiannon Morris, Yuejiang Liu, Elizabeth L. Tang, Sarah Viet, Jason L. Petersen, Philip L. Lorenzi, Lin Tan, Joseph Petrosino, Kristi L. Hoffman, Junya Fujimoto, Seyed Javad Moghaddam, Humam Kadara
Summary: Research has shown that exposure to tobacco carcinogens leads to significant changes in the gut and lung microbiomes, which are associated with the development of lung adenocarcinoma (LUAD) and immunotherapeutic response in human lung cancer patients. Loss of the bacterial growth inhibitor Lcn2 also results in widespread changes in the gut and lung microbiomes. These findings shed light on the role of microbial phenotypes in the development of tobacco-associated LUAD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Mariya V. Gryaznova, Yuliya D. Dvoretskaya, Mikhail Y. Syromyatnikov, Sergey V. Shabunin, Pavel A. Parshin, Evgeniy V. Mikhaylov, Nikolay A. Strelnikov, Vasily N. Popov
Summary: This study investigated the microbiome composition of different sections of the piglet intestine and found that Lactobacillus was the most common genus in the ileum, while Fusobacterium and Bacteroides dominated in the rectum. Comparing the microbiome composition of healthy and diarrheal piglets revealed a decrease in Lactobacillus and an increase in Escherichia-Shigella, Enterococcus, and a decrease in Bacteroides in diarrheal piglets, indicating their contribution to the development of diarrhea.
Article
Gastroenterology & Hepatology
Stefanie Brezina, Martin Borkovec, Andreas Baierl, Fabienne Bastian, Andreas Futschik, Nikolaus Gasche, Thomas Gruenberger, Michael Hallas, Christian Jannsen, Gernot Leeb, Rebecca Lutz, Barbara Sladek, Andrea Gsur
Summary: The study evaluated the usability of FIT cartridges for microbiome analysis and compared it to Stool Collection and Preservation Tubes. The FIT and Preservation Tube samples had highly similar microbiome profiles, with minor differences between abundances of some bacterial taxa. The results indicate that FIT cartridges are suitable for gut microbiome analysis within CRC screening programs.
Article
Microbiology
Mariya Gryaznova, Mikhail Y. Syromyatnikov, Yulia D. Dvoretskaya, Sergey A. Solodskikh, Nikolay T. Klimov, Vitaliy Mikhalev, Vitaliy Zimnikov, Evgeniy Mikhaylov, Vasily N. Popov
Summary: Mastitis is the most common disease for cattle, with recent studies indicating its multi-agent and microbiome diversity. Specific genera such as Staphylococcus and Streptococcus may be associated with subclinical mastitis, while an increase in abundance of Staphylococcus and Microbacterium genera could be an early sign of infection. This study also identified an increase in certain genera like Colidextribacter, Paeniclostridium, and Turicibacter in cows with mastitis, expanding our understanding of the role of the microbiome in this disease.
Article
Nutrition & Dietetics
Inna Burakova, Yuliya Smirnova, Mariya Gryaznova, Mikhail Syromyatnikov, Pavel Chizhkov, Evgeny Popov, Vasily Popov
Summary: Obesity is causing many concomitant diseases, and new strategies for treatment and prevention are being developed using probiotics to modulate the gut microbiota. A study found an increase in Actinobacteriota and a decrease in Bacteroidota in obese patients taking lactic acid bacteria. Beneficial groups like Bifidobacterium and Lactococcus increased, while harmful groups like Faecalibacterium and Clostridium decreased. Modulating the gut microbiota with lactic acid bacteria may be a way to treat obesity.
Review
Microbiology
Sven Kleine Bardenhorst, Tom Berger, Frank Klawonn, Marius Vital, Andre Karch, Nicole Ruebsamen
Summary: Reproducibility in microbiome studies is hindered by a lack of consensus on data analysis strategies. The complex nature of microbiome data, combined with increasingly sophisticated research questions, requires more advanced analytical methods. Current analysis strategies in microbiome studies show significant heterogeneity, particularly in differential abundance testing, indicating a need for better guidance in analyzing increasingly complex research questions.
Review
Gastroenterology & Hepatology
Yinglin Xia
Summary: Mounting evidence has shown a link between gut microbiome and various cancers, but analyzing microbiome data poses challenges and requires proper statistical methods and normalization. This review provides an overview of the unique characteristics of microbiome data and the available normalization methods in microbiome cancer research using examples. The evaluation of these normalization methods is also comprehensively investigated.
Article
Biodiversity Conservation
Barbara Zapala, Justyna Pustelnik, Alicja Dudek, Tomasz Milewicz
Summary: The gastrointestinal microbiota, consisting of trillions of microorganisms, is influenced by lifestyle and diet. A study on healthy Polish individuals following a Western diet found stable oral and gut microbiota profiles, with variations between genders.
Article
Genetics & Heredity
Asuka Morikawa, Ayako Kawabata, Katsuhiko Shirahige, Tetsu Akiyama, Aikou Okamoto, Takashi Sutani
Summary: This study analyzed the association between cervicovaginal microbiome and ovarian cancer, finding that ovarian cancer patients had more diversified microbiota compared to healthy controls, and this was associated with major histotypes of epithelial ovarian cancer. This suggests the potential of cervicovaginal microbiome as a biomarker for screening ovarian cancer in premenopausal women.
Review
Agriculture, Dairy & Animal Science
Margaret D. Weinroth, Aeriel D. Belk, Chris Dean, Noelle Noyes, Dana K. Dittoe, Michael J. Rothrock, Steven C. Ricke, Phillip R. Myer, Madison T. Henniger, Gustavo A. Ramirez, Brian B. Oakley, Katie Lynn Summers, Asha M. Miles, Taylor B. Ault-Seay, Zhongtang Yu, Jessica L. Metcalf, James E. Wells
Summary: Microbiome studies in animal science using 16S rRNA gene sequencing are becoming more common. This review serves as a guide for animal scientists less familiar with this method, discussing common issues and considerations in planning such studies.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Patricia M. Oba, Janelle Kelly, Darcia Kostiuk, Kelly S. Swanson
Summary: Restricted feeding with specially formulated foods can promote safe weight and fat loss, and alter the microbiota of obese dogs. This study demonstrated the effects of controlled feeding on physiological indicators and gut microbiota, proving the effectiveness of restricted feeding in promoting healthy weight loss in obese dogs.
JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Ecology
Nathan G. King, Roberto Uribe, Pippa J. Moore, Hannah S. Earp, Adam Gouraguine, Diego Hinostroza, Alejandro Perez-Matus, Kathryn Smith, Dan A. Smale
Summary: Ecological communities are influenced by processes operating at different spatial scales. Our understanding of microbial biodiversity patterns lags behind that of macro-communities. Bacteria, whether as free-living or associated with host eukaryotes, play important roles in host performance and ecosystem functioning.
Article
Nutrition & Dietetics
Monika Szot, Barbara Fraczek, Florentyna Tyrala
Summary: The aim of this study was to quantify the dietary patterns of Polish esports players aged 18-26 years. Nine distinct dietary patterns were identified, with eight being unhealthy and only one being deemed healthy. Poor dietary habits were observed, including irregular eating of meals, frequent snacking, and unhealthy snack choices. The unbalanced and largely unhealthy dietary habits of esports players in combination with a sedentary lifestyle raise health concerns.
Review
Nutrition & Dietetics
Damian Dynka, Katarzyna Kowalcze, Filip Ambrozkiewicz, Agnieszka Paziewska
Summary: The increasing prevalence of diabetes mellitus has led to a reconsideration of the traditional dietary approach to treating the disease, with a growing interest in the ketogenic diet. This paper provides a comprehensive analysis of the effects of the ketogenic diet on diabetes prevention and treatment, based on a thorough review of the available literature. The findings suggest that the ketogenic diet may have favorable effects on glycated hemoglobin, glucose, insulin, and other metabolic parameters in diabetes patients.
Article
Nutrition & Dietetics
Maria Kulecka, Barbara Fraczek, Aneta Balabas, Pawel Czarnowski, Natalia Zeber-Lubecka, Barbara Zapala, Katarzyna Baginska, Maria Glowienka, Monika Szot, Maciek Skorko, Anna Kluska, Magdalena Piatkowska, Michal Mikula, Jerzy Ostrowski
Summary: This study investigated the composition and function of the gut microbiota in a group of 109 Polish male esports players. The results showed no differences in bacterial diversity, the composition of gut microbiota, or metabolite concentrations between esports players and physical education students. However, there were significant differences in bacterial species and amino acids between esports players and professional athletes.
FRONTIERS IN NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Krzysztof Chmielowiec, Jolanta Chmielowiec, Jolanta Masiak, Aleksandra Stronska-Pluta, Milena Lachowicz, Agnieszka Boron, Dariusz Larysz, Magdalena Dzitkowska-Zabielska, Pawel Cieszczyk, Anna Grzywacz
Summary: DNA methylation is an important epigenetic mechanism that is involved in regulating dopamine release. The study found that nicotine-dependent individuals and athletes had higher levels of DNA methylation compared to the control group.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Phanindra Babu Kasi, Venkata Ramana Mallela, Filip Ambrozkiewicz, Andriy Trailin, Vaclav Liska, Kari Hemminki
Summary: Colorectal cancer (CRC) is a common and fatal disease, with metastasis occurring most frequently in the liver and peritoneum. Conventional treatments have limited efficacy, necessitating the development of new diagnostic and therapeutic approaches. Nanotechnology offers promising nanomaterials for targeted drug delivery and diagnosis in CRC. Combining theranostic approaches with nanomedicine has the potential to revolutionize CRC detection and treatment. This review emphasizes recent studies, potential, and challenges in the development of nanoplatforms for CRC detection and treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Barbara Zapala, Agnieszka Kaminska, Monika Piwowar, Agnieszka Paziewska, Agnieszka Gala-Bladzinska, Ewa L. Stepien
Summary: This study investigated the role of urine-derived extracellular vesicles (uEVs) in diabetic kidney disease (DKD) in patients with type 2 diabetes mellitus (T2DM), identifying specific miRNAs that are enriched or downregulated in T2DM patients with DKD. These miRNAs are involved in cellular processes such as apoptosis, inflammation, and tissue remodeling, and further experimental studies are needed to confirm these findings.
PHARMACEUTICAL RESEARCH
(2023)
Article
Oncology
Karolina Skubisz, Krzysztof Dabkowski, Emilia Samborowska, Teresa Starzynska, Anna Deskur, Filip Ambrozkiewicz, Jakub Karczmarski, Mariusz Radkiewicz, Katarzyna Kusnierz, Beata Kos-Kudla, Tadeusz Sulikowski, Patrycja Cybula, Agnieszka Paziewska
Summary: Pancreatic cancer is a global problem with limited diagnostic and therapeutic options due to a lack of understanding of its pathogenesis. Metabolome analysis revealed unique metabolite profiles for pancreatic ductal adenocarcinoma (PDAC) and neuroendocrine pancreatic tumors (PNET), aiding in the understanding of pancreatic diseases. Disturbed metabolites, including acetylcarnitine C2, serotonin, and glycerophospholipid PC aa C34:1, have the potential to serve as biomarkers for diagnosing and monitoring pancreatic tumors. Serum-circulating metabolites can be easily monitored without invasive procedures, providing valuable information for clinical treatment and dietary strategies.
Article
Microbiology
Beata Lubkowska, Joanna Jezewska-Frackowiak, Michal Sroczynski, Magdalena Dzitkowska-Zabielska, Aleksandra Bojarczuk, Piotr M. M. Skowron, Pawel Cieszczyk
Summary: Currently, Bacillus species bacteria are being used as bacteria concentrates to reduce odor and expel pathogenic bacteria. This study explores the potential of Bacillus species as 'natural' probiotics and evaluates their microbiological characteristics. Industrial microbiological concentrates and their components of mixed Bacillus species cultures were tested, showing promising potential as a bacteria source for food probiotic preparation. Bacillus licheniformis exhibited inhibitory effects on foodborne pathogenic bacteria, while some Bacillus strains showed synergistic effects. Additionally, certain Bacillus strains displayed resistance to tested antibiotics and extended their growth range to thermophilic conditions, making them potential hosts for engineered bionanoparticles construction.
Article
Biochemistry & Molecular Biology
Kinga Huminska-Lisowska, Krzysztof Chmielowiec, Aleksandra Stronska-Pluta, Jolanta Chmielowiec, Aleksandra Suchanecka, Jolanta Masiak, Monika Michalowska-Sawczyn, Agnieszka Boron, Pawel Cieszczyk, Anna Grzywacz
Summary: This study investigated the association between methylation in the promoter region of the DAT1 gene and personality traits in athletes. Results showed that athletes scored significantly higher in extraversion and conscientiousness compared to non-athletes. Total methylation and the number of methylated islands in the DAT1 gene were higher in athletes. Correlations were found between methylation levels and extraversion and agreeability traits. This study provides new insights into the biological mechanisms of dopamine release and personality traits in athletes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Nutrition & Dietetics
Damian Dynka, Katarzyna Kowalcze, Anna Charuta, Agnieszka Paziewska
Article
Nutrition & Dietetics
Hadi Golpasandi, Mohammad Rahman Rahimi, Slahadin Ahmadi, Beata Lubkowska, Pawel Cieszczyk
Summary: The aim of this study was to investigate the combined effects of vitamin D3 supplementation and aerobic training on regulating the autophagy process in rats with type 2 diabetes. The results showed that both aerobic training and vitamin D3 supplementation, either individually or in combination, led to a significant reduction in excessive autophagy and improved aerobic capacity in the rats.
Correction
Microbiology
Beata Lubkowska, Joanna Jezewska-Frackowiak, Michal Sroczynski, Magdalena Dzitkowska-Zabielska, Aleksandra Bojarczuk, Piotr M. Skowron, Pawel Cieszczyk
Article
Cell Biology
Justyna Olszewska, Anna Charuta, Agnieszka Paziewska, Agata Wawrzyniak, Jacek Baj, Grzegorz Teresinski, Grzegorz Buszewicz, Magdalena Kryska
Summary: The skin's most important function is to protect the body from harmful mechanical, physical, and chemical factors. It has the ability to regenerate and repair itself, even after damage caused by tattooing. This research evaluated the structure of the dermis and epidermis using standard histological techniques, and demonstrated significant differences in pigment deposition depth based on age, dermis and epidermis thickness, and tattoo location.
HISTOLOGY AND HISTOPATHOLOGY
(2023)
Article
Sport Sciences
Zhuo Sun, Aleksandra Bojarczuk, Pawel Cieszczyk
Summary: This study assessed the association between COL27A1 rs946053 and COL11A1 rs3753841 SNPs and ACL injury in a cohort of Polish athletes. The results showed no significant associations between the investigated SNPs and ACL injury. However, these findings highlight the importance of further research on the genetic risk mechanisms and etiological factors of ACL injury.
BALTIC JOURNAL OF HEALTH AND PHYSICAL ACTIVITY
(2023)