Article
Multidisciplinary Sciences
Laurie J. Mitchell, Valerio Tettamanti, Justin S. Rhodes, N. Justin Marshall, Karen L. Cheney, Fabio Cortesi
Summary: A gene-editing protocol using the CRISPR/Cas9 system was developed for the false clown anemonefish, resulting in successful targeting of vision and melanin production-related genes with high mutation efficiency. This study demonstrates the potential of CRISPR/Cas9 for studying gene function in anemonefishes and other reef fishes.
Article
Plant Sciences
Abdugaffor Ablazov, Abrar Felemban, Justine Braguy, Hendrik N. J. Kuijer, Salim Al-Babili
Summary: With increasing throughput in both the generation and phenotyping of mutant lines in plants, it is important to have an efficient and reliable genotyping method. We propose an alternative workflow using Phire polymerase and ExoProStar treatment for genotyping mutant plants, which is faster, cheaper, and reduces the risk of contamination and mistakes. The automated sequence analysis systems used in this workflow are accurate and suitable for bulk analysis.
Article
Biotechnology & Applied Microbiology
Francesca Jean, Susan Stasiuk, Tatiana Maroilley, Catherine Diao, Andrew Galbraith, Maja Tarailo-Graovac
Summary: This study demonstrates the high-throughput identification and understanding of intragenic modifiers in a clinically relevant gene by using whole genome sequencing and CRISPR/Cas9, showing that these variants are non-randomly distributed throughout the gene and that genomic context plays a crucial role in phenotypic outcomes.
Article
Plant Sciences
Rasyidah M. Razar, Peng Qi, Katrien M. Devos, Ali M. Missaoui
Summary: Genetic diversity in switchgrass germplasm can be used to increase biomass yield. The study analyzed polymorphism and segregation distortion in two F1 populations and identified QTL for biomass yield. The results showed the presence of QTL in both populations, which explained a significant portion of the phenotypic variation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Yu Fu, Zhenglin Zhu, Geng Meng, Rijun Zhang, Yueping Zhang
Summary: Post-translational modifications of histone proteins play a crucial role in gene expression and cell fate decisions. Developing efficient methods for introducing mutations to histones is vital for studying these modifications. By utilizing a CRISPR-Cas9 based system in yeast cells, researchers successfully generated multiple histone mutants, presenting a valuable tool for the field of epigenetics.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Arvind Sharma, Michael N. Pham, Jeremiah B. Reyes, Randeep Chana, Won C. Yim, Chan C. Heu, Donghun Kim, Duverney Chaverra-Rodriguez, Jason L. Rasgon, Robert A. Harrell, Andrew B. Nuss, Monika Gulia-Nuss
Summary: Ticks have the ability to transmit various debilitating pathogens, but research on ticks has been lacking compared to other arthropod vectors due to challenges in applying genetic and molecular tools. This study reports a successful embryo injection protocol for the black-legged tick and demonstrates the use of CRISPR-Cas9 for genome editing. The results provide innovative tools for tick research.
Article
Plant Sciences
Bicko Steve Juma, Asunta Mukami, Cecilia Mweu, Mathew Piero Ngugi, Wilton Mbinda
Summary: This study successfully developed cassava plants with lower levels of cyanogenic glycoside and cyanide content through CRISPR/Cas9-mediated targeted mutagenesis. This provides an alternative approach for breeding non-toxic cassava varieties.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Fisheries
Qian Li, Hong Yu, Qi Li
Summary: Genome editing using CRISPR/Cas9 methodology holds great potential for improving aquaculture, but the screening and identification of successfully edited individuals remains challenging. In this study, we developed and optimized a high-resolution melting analysis (HRM) assay to rapidly screen and identify CRISPR/Cas9-edited oysters. The HRM assay showed sensitivity in detecting temperature shifts and successfully identified oysters with indel mutations. This reproducible, sensitive, and efficient method has the potential to accelerate genetic improvement in aquaculture.
Article
Biochemistry & Molecular Biology
Me-Sun Kim, Seo-Rin Ko, Yu Jin Jung, Kwon-Kyoo Kang, Yung-Jo Lee, Yong-Gu Cho
Summary: Plants produce stress-resistant substances in response to abiotic stress, and a protein conversion mechanism helps break down damaged proteins. The study focused on the function of OsPUB7 gene in Oryza sativa and found that OsPUB7 plays a role in drought and salinity stress resistance. The interaction between OsPUB7 and other stress-related genes suggests its potential as a target for breeding and future research on drought tolerance in rice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Caixia Gao
Summary: Genome editing has the potential to revolutionize plant breeding and address the challenge of feeding a growing population in the face of climate change. Newly developed techniques and strategies offer significant opportunities for plant improvement and crop production, but challenges must be overcome to realize the full potential of this technology.
Article
Plant Sciences
Yudong Chen, Haiying Xiang, Ling Jia, Qianxu Yang, Jianduo Zhang, Jiarui Jiang, Wanli Zeng, Lele Deng, Jingjing Jin, Qian Gao, Xuemei Li
Summary: A total of 4382 sgRNAs targeting 1060 tobacco genes were obtained, resulting in the creation of 10,682 targeted mutants using high-throughput methods. Four optimization experiments were conducted to address issues encountered during the genetic transformation process.
PLANT CELL REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Ramona Gruetzner, Patrick Martin, Claudia Horn, Samuel Mortensen, Erin J. Cram, Carolyn W. T. Lee-Parsons, Johannes Stuttmann, Sylvestre Marillonnet
Summary: The recent discovery of the mode of action of the CRISPR/Cas9 system has provided biologists with a useful tool for generating site-specific mutations in genes of interest. In this study, optimizing the efficiency of the Cas9 nuclease in generating mutations in target genes by investigating its nucleotide and/or amino acid sequence features led to the finding that adding introns to the Cas9 coding sequence significantly improved editing efficiency. The intronized Cas9 gene was shown to be effective in generating mutant phenotypes in various plants, highlighting the importance of sequence modifications for enhancing CRISPR/Cas9 genome editing.
PLANT COMMUNICATIONS
(2021)
Article
Agronomy
Pasquale Tripodi, Rosa D'Alessandro, Giovanna Festa, Paola Taviani, Roberto Rea
Summary: This study confirmed the uniqueness and distinctness of the 'Peperone Cornetto di Pontecorvo' variety compared to similar pepper types through morpho-agronomic performance, chemical composition analysis, and genomic fingerprinting. The information obtained will be valuable for the recovery, enhancement, and protection of this local variety.
Article
Plant Sciences
Chong Ren, Yanfei Liu, Yuchen Guo, Wei Duan, Peige Fan, Shaohua Li, Zhenchang Liang
Summary: This study identified multiple effective promoters in grapevine and achieved multiplex genome editing by optimizing the CRISPR/Cas9 system, leading to increased editing efficiency. Simultaneous editing of TMT1 and TMT2 genes resulted in reduced sugar levels in grapes, highlighting the role of these genes in sugar accumulation. The findings provide valuable materials for the optimization of CRISPR/Cas9 system and expand the toolbox of grape genome editing for basic research and molecular breeding.
HORTICULTURE RESEARCH
(2021)
Article
Plant Sciences
Mengxi Bi, Zhijun Wang, Keyan Cheng, Yiqing Cui, Yi He, Jian Ma, Mingfang Qi
Summary: This study successfully applied the CRISPR/Cas9 large-scale screening strategy in tomato and constructed a CRISPR/Cas9 mutant library. The study found that homozygous and biallelic mutations occurred at higher frequencies in the mutant population. Additionally, a small-scale CRISPR/Cas9 library enhanced the efficacy of single sgRNA recognition in positive plants.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Food Science & Technology
Yi Ding, Jiajia Huang, Ning Zhang, Soren K. Rasmussen, Dianxing Wu, Xiaoli Shu
JOURNAL OF CEREAL SCIENCE
(2019)
Article
Agronomy
Pernille L. Malik, Luc Janss, Linda K. Nielsen, Finn Borum, Henning Jorgensen, Birger Eriksen, Jan K. Schjoerring, Soren K. Rasmussen
THEORETICAL AND APPLIED GENETICS
(2019)
Article
Plant Sciences
Theresa Ankamah-Yeboah, Lucas Lodewijk Janss, Jens Due Jensen, Rasmus Lund Hjortshoj, Soren Kjrsgaard Rasmussen
FRONTIERS IN PLANT SCIENCE
(2020)
Article
Plant Sciences
Geng Meng, Sabine K. Clausen, Soren K. Rasmussen
Article
Plant Sciences
Shazia Rehman, Bodil Jorgensen, Ejaz Aziz, Riffat Batool, Samar Naseer, Soren K. Rasmussen
Article
Plant Sciences
Ulrika Carlson-Nilsson, Karolina Aloisi, Ingunn M. Vagen, Ari Rajala, Jorgen B. Molmann, Soren K. Rasmussen, Mari Niemi, Ewelina Wojciechowska, Pertti Parssinen, Gert Poulsen, Matti W. Leino
Summary: In the Arctic region of the Nordic countries, peas need to adapt to extreme climate conditions. Research has identified suitable pea genetic resources for cultivation and breeding in the Arctic, considering factors like day length, light spectrum, and temperature. These resources could support the northward expansion of pea cultivation in response to predicted climate changes.
FRONTIERS IN PLANT SCIENCE
(2021)
Editorial Material
Agronomy
Soren K. Rasmussen
Article
Plant Sciences
Geng Meng, Weiyao Fan, Soren K. Rasmussen
Summary: Plant class III peroxidases are involved in essential plant processes, and this study identified and classified 75 carrot peroxidases into seven subgroups based on phylogenetic analysis. The gene structure and conserved motif analyses revealed characteristic features of carrot peroxidases. Tandem duplication was found to be the main contributor to the expansion of this gene family, and RNA sequencing identified candidate genes related to lignification and pigmentation mechanisms.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Agronomy
Da Su, Muhammad Atif Muneer, Xiaohui Chen, Soren K. Rasmussen, Liangquan Wu, Yuanyang Cai, Fangmin Cheng
Summary: This study investigated the influence of nitrogen fertilization on phytic acid concentration and its relation to grain protein fractions in rice. The results showed that nitrogen application decreased phytic acid concentration and increased total protein and grain protein fractions.
Article
Agronomy
Philipp Matthias Steffan, Anders Borgen, Anna Maria Torp, Gunter Backes, Soren K. Rasmussen
Summary: In this study, genome wide association analysis was conducted on 248 wheat varieties for two years to identify marker trait associations for resistance to common bunt. Two significant markers were identified on chromosomes 2B and 7A. This study demonstrates the applicability of genome wide association studies in identifying genetic polymorphisms for resistance to underrepresented plant diseases such as common bunt.
Editorial Material
Plant Sciences
Rafael Tassinari Resende, Karine Chenu, Soren K. Rasmussen, Alexandre Bryan Heinemann, Roberto Fritsche-Neto
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Genetics & Heredity
Geng Meng, Soren K. Rasmussen, Cecilie S. L. Christensen, Weiyao Fan, Anna Maria Torp
Summary: Barley grains are rich in compounds that can be used to develop products for human health, while lignocellulose in straw can be optimized for various purposes. These compounds can be used to breed improved cultivars or combined with mutant lines. The technical demands may vary depending on the intended use of barley.
FRONTIERS IN GENETICS
(2023)
Article
Agronomy
Louise Winther, Soren Kjaersgaard Rasmussen, Gert Poulsen, Conny Bruun Asmussen Lange
Summary: We produced homogeneous lines of 227 pea accessions through single seed descent, and investigated their genetic diversity using microsatellite markers. The results showed high genetic diversity among these mostly Scandinavian accessions. Field experiments revealed variation in seed protein content between different accessions and years, with some accessions showing potential for breeding high-protein pea cultivars.
Article
Biochemistry & Molecular Biology
Yuan Qin, Anna Maria Torp, Gaetan Glauser, Carsten Pedersen, Soren K. Rasmussen, Hans Thordal-Christensen
PLANT SIGNALING & BEHAVIOR
(2019)