Article
Multidisciplinary Sciences
Zhongling Zhuo, Qingchen Wang, Chang Li, Lili Zhang, Lanxin Zhang, Ran You, Yan Gong, Ying Hua, Linzi Miao, Jiefei Bai, Chunli Zhang, Ru Feng, Meng Chen, Fei Su, Chenxue Qu, Fei Xiao
Summary: Tracking abnormal B cells in children with precursor B cell acute lymphoblastic leukemia (pre-B-ALL) is challenging due to sequence variation and immunophenotypic drift. Sequencing the V-(D)-J regions of immunoglobulin and T cell receptor using the Illumina NovaSeq platform provided potential follow-up markers and predictive values for pre-B-ALL children during treatment. NGS-IGH (+) patients had a poor prognosis and consistent CDR3-coded protein structures could be extracted as potential biomarkers for monitoring minimal residual disease (MRD) in pre-B-ALL children.
Article
Multidisciplinary Sciences
Zhuoyi Liang, Lijuan Zhao, Adam Yongxin Ye, Sherry G. Lin, Yiwen Zhang, Chunguang Guo, Hai-Qiang Dai, Zhaoqing Ba, Frederick W. Alt
Summary: Immunoglobulin heavy chain variable region exons are assembled in progenitor -B cells, with VH, D, and JH gene segments located in separate clusters. CTCF-binding elements (CBEs) in the IGCR1 element and 3' Igh-CBEs play a role in segregating different domains and impeding RAG scanning. Downregulation of WAPL in progenitor -B cells neutralizes CBEs and promotes VH-to-DJH rearrangements. The gradual WAPL downregulation mechanism and the reinforcement of CBEs contribute to ordered V(D)J recombination.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Immunology
Justin H. M. Heltzel, Robert W. Maul, William Yang, Patricia J. Gearhart
Summary: This study demonstrates that the distance between the V region and J gene does not affect the extent of mutation. The majority of mutations occur within a 1 kb region downstream of the J gene, regardless of the specific J gene used. This suggests that mutations are targeted by AID interactions with RNA polymerase II.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Immunology
Anastasia O. Smirnova, Anna M. Miroshnichenkova, Laima D. Belyaeva, Ilya V. Kelmanson, Yuri B. Lebedev, Ilgar Z. Mamedov, Dmitriy M. Chudakov, Alexander Y. Komkov
Summary: Diversity of T-cell receptor (TR) variable domains is generated through recombination of alpha (TRA) and beta (TRB) chains. We analyzed poorly explored partial TRBD1-TRBD2 rearrangements in T-cell genomic DNA and detected a bimodal distribution of D-D junctions indicating two active recombination sites. These rearrangements contribute to TRB V-(D)-J combinatorial diversity and are present in human antigen-experienced memory T cells, showing their role in antigen recognition and immune response.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Hematology
Nicholas J. Short, Hagop Kantarjian, Farhad Ravandi, Marina Konopleva, Nitin Jain, Rashmi Kanagal-Shamanna, Keyur P. Patel, Walid Macaron, Tapan M. Kadia, Sa Wang, Jeffrey L. Jorgensen, Joseph D. Khoury, Musa Yilmaz, Partow Kebriaei, Koichi Takahashi, Guillermo Garcia-Manero, Naval Daver, Sean M. Post, Xuelin Huang, Steven M. Kornblau, Sara Pelletier, Wilmer Flores, Jairo Matthews, Rebecca Garris, Elias Jabbour
Summary: This study evaluated the clinical impact of a highly sensitive next-generation sequencing (NGS) MRD assay in acute lymphoblastic leukemia (ALL) patients. The study found that NGS detection could identify patients with a significant risk of relapse, even if they were considered MRD negative by multiparameter flow cytometry (MFC). Early assessment of MRD using NGS provides important prognostic information for predicting relapse risk and long-term survival in ALL patients.
Article
Multidisciplinary Sciences
Louisa Hill, Gordana Wutz, Markus Jaritz, Hiromi Tagoh, Lesly Calderon, Jan-Michael Peters, Anton Goloborodko, Meinrad Busslinger
Summary: By regulating the expression level of Wapl, pro-B cells and pre-B cells use different chromatin folding principles for V gene recombination, leading to allelic exclusion at the Igh locus.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Steven Strubbe, Marieke De Bruyne, Ulrich Pannicke, Elien Beyls, Bart Vandekerckhove, Georges Leclercq, Elfride De Baere, Victoria Bordon, Anne Vral, Klaus Schwarz, Filomeen Haerynck, Tom Taghon
Summary: SCID is a primary deficiency of the immune system caused by genetic defects, leading to fatal infections. Next-generation sequencing has enabled the identification of novel variants in SCID genes, improving diagnostic accuracy and therapy implementation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Xinyue Deng, Meilan Zhang, Jiachen Wang, Xiaoxi Zhou, Min Xiao
Summary: In this study, the distribution of potential prognostic factors in CLL patients was analyzed, revealing a landscape of molecular profiles. The predictive value of recurrent genetic mutations and chromosome aberrations was confirmed, and it was found that IGHJ3 was associated with favorable markers (mutated IGHV, trisomy 12), while IGHJ6 tended to correlate with unfavorable factors (unmutated IGHV, del17p). These results provide an indication for using IGH gene sequencing to predict the prognosis of CLL.
FRONTIERS IN ONCOLOGY
(2023)
Article
Optics
Qianglong Li, Feng Li, Hongjun Liu, Wei Zhao, Hualong Zhao, Yishan Wang, Wenlong Wen, Xue Cao, Jinhai Si
Summary: A compact all-fiber laser source with short pulse durations and excellent performance is demonstrated using fiber quasi-rectangular pulse pre-shaping technique. The laser source is compact, robust, cost-effective, and anticipated to be widely used in industrial applications.
OPTICS AND LASER TECHNOLOGY
(2024)
Article
Chemistry, Applied
B. Yadagiri, K. Narayanaswamy, Ganesh D. Sharma, Surya Prakash Singh
Summary: Two similar V-shaped small molecular non-fullerene acceptors, BYG3 and BYG4, were designed and synthesized for BHJ-OSCs applications, showing good optoelectronic properties. BYG4 exhibited higher short-circuit current density and fill factor compared to BYG3, possibly due to more effective exciton dissociation and charge transfer in the SMD:BYG4 active layer.
Article
Hematology
Muna Qayed, Tao Wang, Michael T. Hemmer, Stephen Spellman, Mukta Arora, Daniel Couriel, Amin Alousi, Joseph Pidala, Hisham Abdel-Azim, Mahmoud Aljurf, Mouhab Ayas, Menachem Bitan, Mitchell Cairo, Sung Won Choi, Christopher Dandoy, David Delgado, Robert Peter Gale, Gregory Hale, Haydar Frangoul, Rammurti T. Kamble, Mohamed Kharfan-Dabaja, Leslie Lehman, John Levine, Margaret MacMillan, David I. Marks, Taiga Nishihori, Richard F. Olsson, Peiman Hematti, Olov Ringden, Ayman Saad, Prakash Satwani, Bipin N. Savani, Kirk R. Schultz, Sachiko Seo, Shalini Shenoy, Edmund K. Waller, Lolie Yu, Mary M. Horowitz, John Horan
BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION
(2018)
Article
Biophysics
Helene M. Schoemans, Stephanie J. Lee, James L. Ferrara, Daniel Wolff, John E. Levine, Kirk R. Schultz, Bronwen E. Shaw, Mary E. Flowers, Tapani Ruutu, Hildegard Greinix, Ernst Holler, Grzegorz Basak, Rafael F. Duarte, Steven Z. Pavletic
BONE MARROW TRANSPLANTATION
(2018)
Article
Allergy
James Slack, Michael H. Albert, Dmitry Balashov, Bernd H. Belohradsky, Alice Bertaina, Jack Bleesing, Claire Booth, Jochen Buechner, Rebecca H. Buckley, Marie Ouachee-Chardin, Elena Deripapa, Katarzyna Drabko, Mary Eapen, Tobias Feuchtinger, Andrea Finocchi, H. Bobby Gaspar, Sujal Ghosh, Alfred Gillio, Luis I. Gonzalez-Granado, Eyal Grunebaum, Tayfun Gungor, Carsten Heilmann, Merja Helminen, Kohei Higuchi, Kohsuke Imai, Krzysztof Kalwak, Nubuo Kanazawa, Gulsun Karasu, Zeynep Y. Kucuk, Alexandra Laberko, Andrzej Lange, Nizar Mahlaoui, Roland Meisel, D. Moshous, Hideki Muramatsu, Suhag Parikh, Srdjan Pasic, Irene Schmid, Catharina Schuetz, Ansgar Schulz, Kirk R. Schultz, Peter J. Shaw, Mary A. Slatter, Karl-Walter Sykora, Shinobu Tamura, Mervi Taskinen, Angela Wawer, Beata Wolska-Kusnierz, Morton J. Cowan, Alain Fischer, Andrew R. Gennery
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2018)
Review
Immunology
Melanie Dieude, Lori J. West, Daniel A. Muruve, Lakshman Gunaratman, Thalachallour Mohanakumar, Emmanuel Zorn, Christopher W. Cairo, Darren H. Freed, Kirk R. Schultz, Robert L. Fairchild, Marie-Josee Hebert
Article
Hematology
Jacob Rozmus, Amina Kariminia, Sayeh Abdossamadi, Barry E. Storer, Paul J. Martin, Stephanie J. Lee, Daniel Wolff, Mukta Arora, Corey Cutler, Kirk R. Schultz
BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION
(2019)
Article
Hematology
Tony H. Truong, Jason D. Pole, Henrique Bittencourt, Tal Schechter, Geoff D. E. Cuvelier, Kristjan Paulson, Meera Rayar, David Mitchell, Kirk R. Schultz, Debbie O'Shea, Randy Barber, Donna Wall, Lillian Sung
BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION
(2019)
Review
Hematology
Stephen Couban, Peggy C. Wong, Kirk R. Schultz
EXPERIMENTAL HEMATOLOGY
(2019)
Article
Hematology
Kirk R. Schultz, Amina Kariminia, Bernard Ng, Sayeh Abdossamadi, Madeline Lauener, Eneida R. Nemecek, Justin T. Wahlstrom, Carrie L. Kitko, Victor A. Lewis, Tal Schechter, David A. Jacobsohn, Andrew C. Harris, Michael A. Pulsipher, Henrique Bittencourt, Sung Won Choi, Emi H. Caywood, Kimberly A. Kasow, Monica Bhatia, Benjamin R. Oshrine, Allyson Flower, Sonali Chaudhury, Donald Coulter, Joseph H. Chewning, Michael Joyce, Sureyya Savasan, Anna B. Pawlowska, Gail C. Megason, David Mitchell, Alexandra C. Cheerva, Anita Lawitschka, Shima Azadpour, Elena Ostroumov, Peter Subrt, Anat Halevy, Sara Mostafavi, Geoffrey D. E. Cuvelier
Letter
Biophysics
Divya Subburaj, Amanda M. Li, Jacob Rozmus, Kirk R. Schultz
BONE MARROW TRANSPLANTATION
(2021)
Article
Biophysics
Ana Zelic Kerep, Atillio Olivieri, Helene Schoemans, Anita Lawitschka, Joerg Halter, Drazen Pulanic, Anne Dickinson, Hildegard T. Greinix, Steven Z. Pavletic, Kirk R. Schultz, Stephanie J. Lee, Daniel Wolff
Summary: The article introduces the formation and content of the cGVHD dictionary, aiming to provide a standardized structure for international databases and facilitate data retrieval and merging.
BONE MARROW TRANSPLANTATION
(2023)
Article
Hematology
Shima Azadpour, Sayeh Abdossamadi, Bernard Ng, Elena Ostroumov, Saeid Abroun, Geoffrey D. E. Cuvelier, Kirk R. Schultz
EXPERIMENTAL HEMATOLOGY
(2023)
Article
Hematology
Sarah M. Bowers, Bernard Ng, Sayeh Abdossamadi, Amina Kariminia, David A. Cabral, Geoffrey D. E. Cuvelier, Kirk R. Schultz, Kelly L. Brown
Summary: Adenosinergic signaling has context-specific effects on immune cells, particularly on lymphocytes. Changes in ADA2 enzyme activity may be associated with the onset of chronic GVHD.
TRANSPLANTATION AND CELLULAR THERAPY
(2023)
Article
Oncology
Chia Huan Ng, Juliana P. Roden, Jefferson Terry, Kirk R. Schultz
Summary: This case report describes three cases of penile chronic GvHD in adolescent male patients who received HSCT before puberty. The symptoms of penile cGvHD became evident during puberty, along with fibrotic changes in the foreskin. Circumcision was required for symptom relief in these patients. The report highlights the importance of frequent monitoring of prepubertal pediatric HSCT patients with sclerotic cGvHD who enter puberty, and the need for optimized systemic and topical immunosuppression treatment.
PEDIATRIC HEMATOLOGY AND ONCOLOGY
(2023)
Article
Hematology
Paul A. Carpenter, Hyoung Jin Kang, Keon Hee Yoo, Marco Zecca, Bin Cho, Giovanna Lucchini, Eneida R. Nemecek, Kirk R. Schultz, Polina Stepensky, Sonali Chaudhury, Benjamin Oshrine, Seong Lin Khaw, Andrew C. Harris, Marta Verna, Liudmila Zubarovskaya, Yihua Lee, Justin Wahlstrom, Lori Styles, Peter J. Shaw, Jean-Hugues Dalle
Summary: Chronic graft-versus-host disease (cGVHD) is a potentially life-threatening complication of allogeneic hematopoietic stem cell transplantation. Clinical data on cGVHD therapies in younger children are limited. This study evaluated the pharmacokinetics, safety, and efficacy of Ibrutinib in children with moderate/severe cGVHD, showing significant activity and acceptable safety.
TRANSPLANTATION AND CELLULAR THERAPY
(2022)
Article
Hematology
Jonathan H. Rayment, Rodrigo A. Sandoval, Juliana P. Roden, Kirk R. Schultz
Summary: This study assessed the feasibility and sensitivity of MBW to detect p-cGvHD in children as young as 3 years after HSCT. Results showed that LCI had high discriminative power for correctly identifying p-cGvHD.
TRANSPLANTATION AND CELLULAR THERAPY
(2022)