Article
Oncology
Manabu Kojima, Kotaro Sugimoto, Makoto Kobayashi, Naoki Ichikawa-Tomikawa, Korehito Kashiwagi, Takafumi Watanabe, Shu Soeda, Keiya Fujimori, Hideki Chiba
Summary: Cell adhesion proteins, including the CLDN family, play a crucial role in maintaining tissue integrity and regulating cellular events. Aberrant expression of CLDN6 is associated with accelerated proliferation and migration in endometrial cancer cells, promoting tumor growth and invasion. The CLDN6/SFK/PI3K/AKT and SGK signaling pathways activate ERα in an ERα-independent manner, contributing to tumor progression. Moreover, CLDN6 can also regulate gene expression independently of ERα.
MOLECULAR CANCER RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Laura Stein, Nora Brunner, Salah Amasheh
Summary: The epithelial barrier plays a crucial role in gastrointestinal function by preventing the passage of solutes. This study investigates the interaction between claudin-4 and claudin-18.2, two tight junction proteins in the gastric epithelium, and suggests that this interaction is dependent on both mechanical force and pH. The findings highlight the importance of tight junction integrity for proper gastrointestinal function.
Article
Biochemistry & Molecular Biology
Viviana Scalavino, Emanuele Piccinno, Antonio Lacalamita, Angela Tafaro, Raffaele Armentano, Gianluigi Giannelli, Grazia Serino
Summary: This study identified 18 dysregulated miRNAs in intestinal epithelial cells from the ulcerative colitis (UC) mice model. MiR-195-5p, one of the down-regulated miRNAs, was found to be involved in impaired barrier function. The study demonstrated that miR-195-5p can regulate tight junctions by decreasing CLDN2 levels and subsequently affecting CLDN1 expression.
Review
Biochemistry & Molecular Biology
Rina Fujiwara-Tani, Shiori Mori, Ruiko Ogata, Rika Sasaki, Ayaka Ikemoto, Shingo Kishi, Masuo Kondoh, Hiroki Kuniyasu
Summary: CLDN4 is a crucial component of tight junctions in epithelial cells and is overexpressed in many epithelial malignancies. Changes in CLDN4 expression are associated with epigenetic factors, inflammation, and growth factor signaling. CLDN4 plays a role in maintaining the tumor microenvironment and acts as a barrier to anticancer drug entry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Linda Droessler, Valeria Cornelius, Alexander G. Markov, Salah Amasheh
Summary: The study demonstrates the detrimental effects of TNFα on non-transformed intestinal epithelial cells, leading to barrier dysfunction. This is achieved through modulation of tight junction proteins and TNF receptors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Kazuto Tsukita, Manabu Kitamata, Hiroka Kashihara, Tomoki Yano, Ikuko Fujiwara, Timothy F. Day, Tatsuya Katsuno, Jaewon Kim, Fumiko Takenaga, Hiroo Tanaka, Sungsu Park, Makoto Miyata, Hitomi Watanabe, Gen Kondoh, Ryosuke Takahashi, Atsushi Tamura, Sachiko Tsukita
Summary: Liquid-liquid phase separation (LLPS) plays a role in dynamic biological phenomena. In this study, the role of LLPS in the dynamic regulation of junctional actin filaments in epithelial cells was investigated. The researchers identified a multifunctional actin nucleator called cordon bleu (Cobl) as an AJC-enriched microtubule-associated protein and conducted comprehensive in vitro and in vivo analyses. They found that apical microtubules promoted LLPS of Cobl at the AJC, leading to increased Cobl actin assembly activity and spatiotemporal regulation of junctional actin assembly for epithelial morphogenesis and paracellular barriers.
Article
Physiology
Linda Droessler, Valeria Cornelius, Elisa Boehm, Laura Stein, Nora Brunner, Salah Amasheh
Summary: TNF affects the barrier function of follicle-associated epithelium of porcine Peyer's patches, but has no effects on villus epithelium. These findings reveal the differences in epithelial barrier function between the two structures and the importance of Peyer's patches as a primary mucosal immune defense.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Food Science & Technology
Marta Justyna Koziel, Maksymilian Ziaja, Agnieszka Wanda Piastowska-Ciesielska
Summary: The intestinal barrier acts as the main defense against substances entering the body, ensuring balance in the organism; Mycotoxins, toxic fungi metabolites, may disrupt homeostasis by affecting the intestinal barrier; Claudins, proteins involved in tight junction formation between epithelial cells, play a significant role in responding to mycotoxin-induced cytotoxicity.
Article
Immunology
Mitsunobu Ueda, Hidetomo Kobayashi, Soshi Seike, Eizo Takahashi, Keinosuke Okamoto, Hiroyasu Yamanaka
Summary: Aeromonas sobria, a Gram-negative pathogen causing food-borne illness, opportunistically leads to severe extraintestinal diseases in immunocompromised patients and the elderly. It can disrupt the barrier function of the intestinal epithelium by destroying tight junctions, potentially aiding the invasion of pathogens into deep sites in the human body.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Cell Biology
Shaista Anwer, Emily Branchard, Qinghong Dan, Angela Dan, Katalin Szaszi
Summary: Claudins are crucial for tight junction formation and paracellular transport. TNFa can impact epithelial permeability through modulating the expression of specific claudins, including claudin-3. The effect of TNFa on claudin-3 is mediated by ERK-dependent activation of NF-kappa B and PKA-induced activation of CREB1 pathways, both of which are necessary for the upregulation of claudin-3.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Review
Medicine, General & Internal
Spyridoula Katsarou, Michael Makris, Efstratios Vakirlis, Stamatios Gregoriou
Summary: This systematic review focuses on the role of tight junctions in atopic dermatitis and their potential impact on therapeutic approaches. The findings suggest that dysfunctional tight junctions are closely associated with the pathogenesis and inflammation in atopic dermatitis, and further investigation into their functionality may lead to improved epidermal barrier function in this condition.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
Nicolas Obtel, Adeline Le Cabec, The Nghia Nguyen, Eloise Giabicani, Stijn J. M. Van Malderen, Jan Garrevoet, Aline Percot, Celine Paris, Christopher Dean, Smail Hadj-Rabia, Pascal Houillier, Tilman Breiderhoff, Claire Bardet, Thibaud Coradin, Fernando Ramirez Rozzi, Catherine Chaussain
Summary: Claudin proteins are crucial for determining the permeability and ion specificity of tight junctions in epithelia. Deficiency in claudin-10 is associated with severe enamel wear in HELIX syndrome patients. Analysis of a HELIX patient's molar revealed accentuated strontium variations in the enamel and dentin, possibly indicating episodes of worsening renal dysfunction.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Article
Endocrinology & Metabolism
Lars Winkler, Rosel Blasig, Olga Breitkreuz-Korff, Philipp Berndt, Sophie Dithmer, Hans C. Helms, Dmytro Puchkov, Kavi Devraj, Mehmet Kaya, Zhihai Qin, Stefan Liebner, Hartwig Wolburg, Anuska Andjelkovic, Andre Rex, Ingolf E. Blasig, Reiner F. Haseloff
Summary: The study shows that Cldn3 and occludin play a protective role in the stroke by maintaining the integrity of TJ and BBB, but also contribute to the development of edema and infarction.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2021)
Article
Cell Biology
Soyoung Hwang, Peter Chang-Whan Lee, Dong Min Shin, Jeong Hee Hong
Summary: Spinophilin plays a crucial role in modulating the junctional network and cellular migration in lung cancer cells, affecting invasive ability and transporter activity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Juan Huang, Haolin Zhou, Tao Song, Bing Wang, Hongda Ge, Demeng Zhang, Peili Shen, Xia Qiu, Huajun Li
Summary: This study explored the protective properties of fecal microbiota derived from mice treated with sodium alginate (SA) and normal mice, both immunosuppressed by antibiotics and cyclophosphamide. SA intervention improved the diversity and composition of gut microbiota in normal mice. Fecal microbiota transfer (FMT) from SA-treated and normal mice reduced tissue damage, improved immune function, alleviated intestinal mucosal injury, and reduced gut inflammation. FMT also normalized gut microbiota and down-regulated inflammatory pathways. This research highlights the potential of SA and FMT in mitigating intestinal barrier damage and gut dysbiosis in immunosuppressed mice.
Article
Toxicology
Karolina Kowalska, Marta Justyna Koziel, Dominika Ewa Habrowska-Gorczynska, Kinga Anna Urbanek, Kamila Dominska, Agnieszka Wanda Piastowska-Ciesielska
Summary: The PI3K/Akt signaling pathway is one of the most deregulated pathways in prostate cancer, and DON can affect apoptosis and autophagy in normal prostate epithelial cells by modulating this pathway. High concentrations of DON induce apoptosis, while low concentrations induce autophagy.
ARCHIVES OF TOXICOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kamila Dominska, Kinga Anna Urbanek, Karolina Kowalska, Dominika Ewa Habrowska-Gorczynska, Marta Justyna Koziel, Tomasz Ochedalski, Agnieszka Wanda Piastowska-Ciesielska
Summary: This study investigated the effects of angiotensin peptides on HGSOC cell lines and found that these peptides can impact cell proliferation, motility, and cell cycle distribution. However, they do not significantly affect the expression of genes associated with epithelial-mesenchymal transition. Furthermore, the effects of angiotensin peptides on cancer cells depend on the availability of growth factors and nutrients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Agnieszka Pawlos, Marlena Broncel, Ewelina Wozniak, Lukasz Markiewicz, Agnieszka Piastowska-Ciesielska, Paulina Gorzelak-Pabis
Summary: This study aimed to evaluate the impact of SGLT2 inhibitors on the integrity of endothelial cells disrupted by 25-OHC and assess the association with changes in VE-cadherin levels. The results showed that SGLT2 inhibitors, when used in conjunction with 25-OHC, significantly increased endothelial integrity and improved the integrity of endothelial cells disrupted by 25-OHC. This effect was associated with significant improvements in VE-cadherin levels.
Article
Biochemistry & Molecular Biology
Kinga Anna Urbanek, Karolina Kowalska, Dominika Ewa Habrowska-Gorczynska, Marta Justyna Koziel, Kamila Dominska, Agnieszka Wanda Piastowska-Ciesielska
Summary: This study evaluates the impact of AOH on the local steroidogenesis process in normal and prostate cancer cells. The findings suggest that while AOH has a significant impact on cell cycle, inflammation, and apoptosis in prostate cancer cells, its influence on steroidogenesis is not significant. However, when combined with another steroidogenic agent, AOH significantly affects steroidogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Marta Justyna Koziel, Agnieszka Wanda Piastowska-Ciesielska
Summary: Ovarian cancer, a common cancer in women, is believed to be influenced by hormonal imbalance. Estrogens, a group of hormones with important roles in physiological and pathological processes, are involved in regulating proliferation, invasiveness, and the epithelial to mesenchymal transition in ovarian cancer. Estrogen signaling also plays a role in the biology of the tumor microenvironment. This review provides information on estrogen receptors, estrogens, and their association with the tumor microenvironment, as well as changes in estrogen receptor expression following exposure to various environmental chemicals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Toxicology
Marta Justyna Koziel, Dominika Ewa Habrowska-Gorczynska, Kinga Anna Urbanek, Kamila Dominska, Agnieszka Wanda Piastowska-Ciesielska, Karolina Kowalska
Summary: This study found that alternariol may affect ovarian cancer cells by modulating estrogen receptor alpha, leading to apoptosis, oxidative stress, and decreased invasive ability.
TOXICOLOGY LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Magdalena Kluska, Agnieszka Wanda Piastowska-Ciesielska, Paulina Tokarz
Summary: Cancer cells can temporarily arrest their cell cycle at the G0 or G1 phase, which may contribute to tumor heterogeneity and drug resistance. The influence of G0- and G1-arrest on chemosensitivity has not been fully understood.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Oncology
Beata Paulina Rurarz, Kinga Anna Urbanek, Urszula Karczmarczyk, Joanna Raczkowska, Dominika Ewa Habrowska-Gorczynska, Marta Justyna Koziel, Karolina Kowalska, Slawomir Kadlubowski, Agnieszka Sawicka, Michal Maurin, Agnieszka Wanda Piastowska-Ciesielska, Piotr Ulanski
Summary: By utilizing nanomedicine and nuclear medicine, we have developed a radioisotope nanocarrier for the treatment of prostate cancer. This nanocarrier can specifically target prostate cancer cells and exhibits excellent performance in vitro with high stability. Although further studies are required to validate its efficacy in vivo, our research represents a significant advancement in the field of radionanomedicine.
