Article
Microbiology
Zengfeng Zhang, Jiang Chang, Xuebin Xu, Mengjun Hu, Shoukui He, Xiaojie Qin, Min Zhou, Chunlei Shi, Xianming Shi
Summary: Salmonella enterica serovar Indiana has become increasingly prevalent in China as a foodborne pathogen. This study investigated the national prevalence and genetic characterization of this pathogen. The study found that the ST17 clone of S. Indiana is widely distributed in China, and is highly resistant to multiple antibiotics. The genetic analysis revealed the close relationship between ST17 isolates in China and those from other countries. These findings highlight the importance of surveillance and control measures against the high-resistant ST17 clone.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Izumo Kanesaka, Akira Ohno, Masahiro Morita, Akiko Kanayama Katsuse, Takefumi Morihana, Takamitsu Ito, Hiroshi Takahashi, Intetsu Kobayashi
Summary: This study reveals that the PenA mosaic sequence in ceftriaxone-resistant Neisseria gonorrhoeae FC428 clone originated from oral commensal Neisseria species, especially N. subflava. The expression of extended-spectrum cephalosporin resistance is influenced by one of the PenA FC428-like sequence gene polymorphisms, while the majority of PenA FC428 mosaic sequences are involved in regulating the resistance expression without directly contributing to the resistance level itself.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Multidisciplinary Sciences
Naris Kueakulpattana, Dhammika Leshan Wannigama, Sirirat Luk-in, Parichart Hongsing, Cameron Hurst, Vishnu Nayak Badavath, Piroon Jenjaroenpun, Thidathip Wongsurawat, Nipat Teeratakulpisan, Stephen J. Kerr, Shuichi Abe, Phatthranit Phattharapornjaroen, Aye Mya Sithu Shein, Thammakorn Saethang, Naphat Chantaravisoot, Mohan Amarasiri, Paul G. Higgins, Tanittha Chatsuwan
Summary: The emergence of azithromycin/ceftriaxone resistant Neisseria gonorrhoeae in Thailand underscores the global threat to current treatment regimens, highlighting the importance of vigilant surveillance.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Jennifer Roeder, Pascal Felgner, Michael Hensel
Summary: Salmonella enterica Typhimurium obtains nutrients required for rapid proliferation by forming Salmonella-induced filaments and expressing nutrient uptake systems, adapting uniquely to different host cell nutrient conditions. This leads to improved nutrient supply and bacterial proliferation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Jaclyn A. Cusumano, Ruhma Khan, Zeel Shah, Cassie Philogene, Amrita Harrichand, Vanthida Huang
Summary: Penicillin plus ceftriaxone demonstrates similar synergistic activity against Enterococcus faecalis to ampicillin plus ceftriaxone. Isolates with a penicillin MIC of 4 mg/L and a ceftriaxone MIC of 512 or higher lack penicillin plus ceftriaxone synergy despite the penicillin susceptibility MIC breakpoint of 8 mg/L.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biotechnology & Applied Microbiology
Sadanand Dangari Akshay, Karanth Padyana Anupama, Vijaya Kumar Deekshit, Anusha Rohit, Biswajit Maiti
Summary: This study evaluated the expression of outer membrane proteins (OMPs) in Salmonella Typhi at sub-lethal concentrations of ceftriaxone. The results revealed the potential involvement of OMPs in the drug resistance of S. Typhi, providing insights for the development of therapeutic strategies against multi-drug resistant isolates of S. Typhi.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Immunology
Po-Yu Liu, Kung-Ching Wang, Yu-Ping Hong, Bo-Han Chen, Zhi-Yuan Shi, Chien-Shun Chiou
Summary: The first imported case of XDR typhoid fever in Taiwan is linked to a bacterial strain closely related to Pakistan. Treatment for XDR typhoid fever should involve a combination of meropenem and an antimicrobial with intracellular activity against Salmonella.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2021)
Article
Biotechnology & Applied Microbiology
Eman Ali, Gisele LaPointe
Summary: The hydrolysis of milk proteins produces bioactive peptides that can decrease the expression of virulence genes in Salmonella enterica subsp. enterica typhimurium. Five synthetic peptides derived from milk showed antivirulence activity and exhibited a synergistic interaction in downregulating the expression of virulence genes.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2022)
Article
Microbiology
Sushmita Sridhar, Sally Forrest, Derek Pickard, Claire Cormie, Emily A. Lees, Nicholas R. Thomson, Gordon Dougan, Stephen Baker
Summary: Antimicrobial resistance is a critical global health crisis, with rising resistance to fluoroquinolones such as ciprofloxacin. Research has shown that clinical isolates of Salmonella enterica serovar Typhimurium adapt to high concentrations of ciprofloxacin in a way that promotes intracellular survival, with different isolates responding heterogeneously to the drug.
Article
Microbiology
Lara Thieme, Simon Briggs, Eamon Duffy, Oliwia Makarewicz, Mathias W. Pletz
Summary: This study suggests that benzylpenicillin/ceftriaxone is suitable for the treatment of E. faecalis infections with ceftriaxone MIC ≤ 256mg/L, but not for those with ceftriaxone MIC ≥ 512mg/L. Further investigation is needed to analyze the relationship between ceftriaxone susceptibility and penicillin/ceftriaxone synergy.
Article
Infectious Diseases
Mami Hanao, Kotaro Aoki, Yoshikazu Ishii, Ken Shimuta, Makoto Ohnishi, Kazuhiro Tateda
Summary: In this study, 55 strains of Neisseria gonorrhoeae collected from hospitals in Japan were characterized, with one strain identified to have acquired ceftriaxone resistance due to a mutant PBP2 mosaic type incorporating partial sequences from commensal Neisseria species. This finding highlights a potential mechanism for the emergence of ceftriaxone-resistant strains.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Meiling Liu, Kunpeng Zhu, Xinge Li, Yiran Han, Chaojie Yang, Hongbo Liu, Xinyin Du, Xuebin Xu, Haiyan Yang, Hongbin Song, Shaofu Qiu, Ying Xiang
Summary: This study investigated the resistance mechanism of a Salmonella Typhimurium strain isolated from an infant's faecal sample, which exhibited resistance to multiple antibiotics. Through whole genome sequencing, multilocus sequence typing, and plasmid analysis, it was found that the strain carried multiple antimicrobial resistance genes and plasmids.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Agriculture, Dairy & Animal Science
Xin Yu, Hongwei Zhu, Yongheng Bo, Youzhi Li, Yue Zhang, Yang Liu, Jianlong Zhang, Linlin Jiang, Guozhong Chen, Xingxiao Zhang
Summary: The study investigated the prevalence and antimicrobial resistance of Salmonella enterica serovar Enteritidis in broiler chickens in Shandong Province, China. It found that S. Enteritidis was the predominant serovar with high antimicrobial resistance rates to several antibiotics. Multidrug resistance, particularly to fluoroquinolone, showed an increasing trend from 2013 to 2018. This study provides updated information on antimicrobial resistance data and risk assessment for Salmonella Enteritidis in broiler chickens in Shandong Province, which can be valuable for future surveillance activities to ensure chicken supply safety.
Article
Microbiology
Shannon G. Murphy, Andrew N. Murtha, Ziyi Zhao, Laura Alvarez, Peter Diebold, Jung-Ho Shin, Michael S. VanNieuwenhze, Felipe Cava, Tobias Doerr
Summary: In Vibrio cholerae, endopeptidases are essential for proper cell elongation and division, while aPBPs become crucial for maintaining structural integrity during endopeptidase insufficiency, and the Rod system remains active but contributes minimally to cell expansion under these conditions.
Article
Biochemistry & Molecular Biology
Jiao Pan, Weiyi Li, Jiahao Ni, Kun Wu, Iain Konigsberg, Caitlyn E. Rivera, Clayton Tincher, Colin Gregory, Xia Zhou, Thomas G. Doak, Heewook Lee, Yan Wang, Xiang Gao, Michael Lynch, Hongan Long
Summary: This study provides a systematic understanding of mutation rates and molecular spectra in a pathogen, showing low mutation rates and high genome stability. The study also suggests that elevated mutation rates may not be the major determinant for the diversification of toxin and secretion systems, and highlights the potential differences in accuracy between replication and transcription machinery.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Article
Biochemistry & Molecular Biology
Juanjo Huesa, Joaquin Giner-Lamia, M. Graciela Pucciarelli, Francisco Paredes-Martinez, Francisco Garcia-del Portillo, Alberto Marina, Patricia Casino
Summary: RcsB is a transcriptional regulator in enteric bacteria that controls gene expression through binding to target gene promoters in an active dimeric conformation, with DNA binding potentially stabilizing an active conformation in unphosphorylated RcsB. Phosphorylation contributes to gene regulation and RcsB may play a role in controlling iron metabolism. The RcsB box for active dimeric binding to DNA was identified as TN(G/A)GAN(4)TC(T/C)NA, found in promoter, intergenic, and intragenic regions to facilitate both increased or decreased gene transcription.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Microbiology
Juan J. Cestero, Sonia Castanheira, M. Graciela Pucciarelli, Francisco Garcia-del Portillo
Summary: Horizontal gene transfer has played a significant role in the evolution of Salmonella enterica as a pathogen, with specific proteins like ScwA regulating peptidoglycan metabolism and influencing the pathogen's virulence in the host. Deficiency of ScwA leads to a hypervirulent phenotype in murine models, indicating its importance in ensuring cell envelope homeostasis along the infection process.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Johnny Lisboa, Cassilda Pereira, Aline Rifflet, Juan Ayala, Mateus S. Terceti, Alba V. Barca, Ines Rodrigues, Pedro Jose Barbosa Pereira, Carlos R. Osorio, Francisco Garcia-del Portillo, Ivo Gomperts Boneca, Ana do Vale, Nuno M. S. dos Santos
Summary: Peptidoglycan (PG) is a crucial component of the bacterial cell wall, and the enzyme PnpA produced by Phdp can cleave PG from competing bacteria, aiding Phdp in gaining a competitive advantage.
Article
Biotechnology & Applied Microbiology
Charlotte Dessaux, M. Graciela Pucciarelli, Duarte N. Guerreiro, Conor P. O'Byrne, Francisco Garcia-Del Portillo
Summary: The response to stress mediated by the alternative sigma factor SigB has been extensively studied in Listeria monocytogenes when adapting to an intracellular lifestyle. Despite the absence of the core stressosome protein RsbS, SigB activity still increases gradually inside host cells. The unique configuration of the stressosome in intracellular bacteria challenges current models and suggests a sophisticated response to environmental stress cues.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Maria Laura Echarren, Nicolas R. Figueroa, Luisina Vitor-Horen, M. Graciela Pucciarelli, Francisco Garcia-del Portillo, Fernando C. Soncini
Summary: This study identified a novel link between biofilm formation and Salmonella virulence, showing that MlrB represses CsgD expression inside host cells and suggests that this repression decreases the activation of cellulose synthase. These findings provide new insight into the pathogenic mechanisms of Salmonella.
MOLECULAR MICROBIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Victoria Fernandez-Garcia, Silvia Gonzalez-Ramos, Paloma Martin-Sanz, Francisco Garcia-del Portillo, Jose Moises Laparra, Lisardo Bosca
Summary: NOD1 is a pattern recognition receptor that detects bacterial peptidoglycan fragments and other danger signals, linked to inflammatory pathologies. Dysbiosis can alter tissues and organs homeostasis, and the contribution of NOD1 to metabolic disorders like obesity, diabetes, and cardiovascular diseases requires further investigation.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Microbiology
Ariadna Fernandez-Calvet, Begona Euba, Celia Gil-Campillo, Arancha Catalan-Moreno, Javier Moleres, Sara Marti, Alexandra Merlos, Jeroen D. Langereis, Francisco Garcia-del Portillo, Lauren O. Bakaletz, Garth D. Ehrlich, Eric A. Porsch, Margarita Menendez, Joshua C. Mell, Alejandro Toledo-Arana, Junkal Garmendia
Summary: This study reveals the molecular mechanisms of NTHi pathoadaptation based on within-patient lifestyle switching controlled by phase variation. Adhesins such as HMW1A in NTHi display allelic variation, driving changes in epithelial invasion phenotype over time. This highlights the importance of bacterial adaptation in chronic infections and the role of genetic variants in shaping bacterial behavior.
Article
Microbiology
Ana H. Oliveira, Teresa Tiensuu, Duarte N. Guerreiro, Hasan Tukenmez, Charlotte Dessaux, Francisco Garcia-del Portillo, Conor O'Byrne, Jorgen Johansson
Summary: This study investigates the role of the RsbX protein in modulating the activity of the stressosome and regulating SigB activity. The results show that RsbX reduces SigB activation levels under nonstress conditions and is required for appropriate SigB-mediated stress adaptation.
JOURNAL OF BACTERIOLOGY
(2022)
Review
Immunology
Juan J. Quereda, Alvaro Moron-Garcia, Carla Palacios-Gorba, Charlotte Dessaux, Francisco Garcia-del Portillo, M. Graciela Pucciarelli, Alvaro D. Ortega
Summary: Listeria monocytogenes is a ubiquitous bacterium capable of causing listeriosis in humans and animals, due to its exceptional ability to adapt to various stress conditions. It colonizes and disseminates in the host, leading to various diseases, especially in immunocompromised individuals, the elderly, and pregnant women. The pathogen uses unique strategies to invade host cells and spread, presenting challenges to food safety and public health.
Article
Infectious Diseases
Juan J. Cestero, Sonia Castanheira, Henar Gonzalez, Oscar Zaragoza, Francisco Garcia-Del Portillo
Summary: The study aims to find compounds with high affinity for PBP3(SAL) to control Salmonella intracellular infections. A library of 1520 approved drugs was screened using a Salmonella Delta PBP3 mutant and its wild-type strain to identify compounds that caused filamentation in the mutant but not in the wild-type strain. The cephalosporin cefotiam exhibited higher affinity for PBP3(SAL) and showed therapeutic value in treating Salmonella intracellular infections.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Biology
Sonia Castanheira, Francisco Garcia-del Portillo
Summary: Cell shape is genetically inherited and controlled by a universal system of peptidoglycan synthases guided by cytoskeletal scaffolds. This study reveals the existence of two elongasomes that generate rod-shaped morphology in the same bacterium, Salmonella. These elongasomes can be purified independently, respond to different environmental cues, and are directed by distinct peptidoglycan synthases.
COMMUNICATIONS BIOLOGY
(2023)