期刊
ACS SENSORS
卷 5, 期 6, 页码 1650-1656出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.0c00252
关键词
bacterial pathogens; 16S rRNA; dual-mode detection; thermally activated delayed fluorescence; time-resolved fluorescence imaging
资金
- National Natural Science Foundation of China (NSFC) [21778077, 21807116]
- Sino-German research project [GZ 1271]
- Drug Innovation Major Projects [2018ZX09711-001-008]
- CAMS Innovation Fund for Medical Sciences [CIFMS 2017-I2M-2-004]
- Disciplines Construction Project [201920200805]
The specific detection of pathogens has long been recognized as a vital strategy for controlling bacterial infections. Herein, a novel hydrophilic aromatic-imide-based thermally activated delayed fluorescence (TADF) probe, AI-Cz-Neo, is designed and synthesized by the conjugation of a TADF emitter with a bacterial 16S ribosomal RNA-targeted moiety, neomycin. Biological data showed for the first time that AI-Cz-Neo could be successfully applied for the dual-mode detection of bacterial 16S rRNA using confocal fluorescence imaging and time-resolved fluorescence imaging (TRFI) in both cells and tissues. These findings greatly expand the application of TADF fluorophores in time-resolved biological imaging and provide a promising strategy for the precise and reliable diagnosis of bacterial infections based on the dual-mode imaging of bacterial 16S rRNA by fluorescence intensity and fluorescence lifetime.
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