期刊
ACS SENSORS
卷 5, 期 4, 页码 1216-1222出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.0c00292
关键词
electrochemiluminescence biosensor; nucleolin; single-cell detection; silica nanoparticles; synergetic apoptosis
资金
- National Natural Science Foundation of China [21605069]
- China Postdoctoral Science Foundation [2016M600518]
- Taishan Scholar Foundation of Shandong Province [ZR2017ZC0226]
Nucleolin, a nuclear biological multifunctional protein, plays significant roles in modulating the proliferation, survival, and apoptosis of tumor cells. Different from the traditional electrochemiluminescence (ECL) method, a new ECL biosensor was built to perform ECL imaging of nucleolin in a single HeLa cell with high sensitivity and throughput. Briefly, mesoporous silica nanoparticles (MSN) loaded with doxorubicin (DOX) and phorbol 12-myristate 13-acetate (PMA) were used as drug carriers and could be specifically opened by nucleolin in a HeLa cell. PMA then induced the HeLa cell to produce reactive oxygen species (ROS) and realized ECL imaging of nucleolin. After that, ROS could damage DNA and proteins of the tumor cell and DOX could induce the apoptosis of HeLa cells by inhibiting genetic material, nucleic acid, synthesis. HeLa cells were then efficiently killed by DOX and ROS in a synergetic pathway. Herein, a new ECL biosensor for ECL imaging of nucleolin in a single HeLa cell and synergetic tumor therapy was built.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据