Article
Biochemistry & Molecular Biology
Emanuel Moisa, Madalina Dutu, Dan Corneci, Ioana Marina Grintescu, Silvius Negoita
Summary: Bacterial and viral sepsis have distinct hematological patterns, with procalcitonin and RDW% showing the best discriminatory ability between them. Procalcitonin is also strongly correlated with disease severity in both types of sepsis. Other hematological parameters, such as leukocytes, monocytes, and neutrophils, also show good discriminative ability between sepsis types. However, some hematological parameters have fair or no discriminative ability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Megha Srinivas, Preeti Sharma, Siddharth Jhunjhunwala
Summary: Research findings suggest that immune cells can phagocytose particles under constant fluidic flow. Different cell types show similar or better uptake rates under flow conditions. Even in the presence of crowded red blood cells, neutrophils and monocytes are able to uptake particles while flowing.
MOLECULAR PHARMACEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Zhiguo Gong, Shuangyi Zhang, Baichen Gu, Jinshan Cao, Wei Mao, Yuan Yao, Jiamin Zhao, Peipei Ren, Kai Zhang, Bo Liu
Summary: This study demonstrated that Codonopsis pilosula polysaccharides (CPPS) have a protective effect on acute lung injury (ALI) induced by lipopolysaccharide (LPS) and Escherichia coli (E. coli) infection. CPPS can reduce the secretion of inflammatory molecules and the activation of signaling pathways, enhance bacterial phagocytosis, and inhibit bacterial growth. In addition, CPPS can attenuate lung damage in mice, reducing the production of tissue damage biomarkers.
Article
Biochemistry & Molecular Biology
Zhiyi Ding, Feifei Du, Richard Garland Averitt, Gabriel Jakobsson, Carl-Fredrik Ronnow, Milladur Rahman, Alexandru Schiopu, Henrik Thorlacius
Summary: Blocking S100A9 function can attenuate neutrophil infiltration and edema formation in sepsis-induced lung injury, inhibit the up-regulation of Mac-1 on neutrophils, and decrease the levels of CXC chemokines in circulation and lungs. These findings suggest that targeting S100A9 could be a useful strategy to protect against excessive inflammation and lung damage in sepsis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Zhi-Bin Huang, Zhen Hu, Chen-Xin Lu, Si-Dan Luo, Yu Chen, Zhi-Peng Zhou, Jing-Juan Hu, Fang-Ling Zhang, Fan Deng, Ke-Xuan Liu
Summary: This study reveals the protective role of gut microbiota-derived indole 3-propionic acid (IPA) in sepsis. IPA promotes macrophage phagocytosis to control infection and improve clinical outcomes in septic patients. The findings suggest that IPA may be a potential new treatment strategy for sepsis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biology
Ivo Fabrik, Orsolya Bilkei-Gorzo, Maria Oberg, Daniela Fabrikova, Johannes Fuchs, Carina Sihlbom, Melker Goransson, Anetta Hartlova
Summary: Resident tissue macrophages are specialized phagocytes responsible for maintaining tissue homeostasis. A quantitative proteomics approach identified cathepsins as key determinants of phagosome maturation in different tissues. Cathepsin K (CtsK) was found to be a molecular marker in lung phagosomes, required for intracellular protein and collagen degradation. Inhibition of CtsK activity reduced phagosomal proteolysis and collagenolysis in lung-resident macrophages. These findings highlight the importance of CtsK in lung phagosomes and its role in phagocytic collagen clearance in the lungs.
LIFE SCIENCE ALLIANCE
(2023)
Article
Fisheries
Shun Yang, Yuanxin Ma, Xiaocong Lou, Zhewei Zhou, Huimin Zhang, Shunfa Yi, Yan Cheng, Shichao Qian, Mengmeng Huang, Hui Fei
Summary: The phagocytic capacity of largemouth bass leukocytes, particularly granulocytes/monocytes, was observed against fluorescent microspheres and Aeromonas hydrophila. Up-regulation of phagocytosis-related genes and TNF-alpha was observed in the leukocytes following A. hydrophila stimulation. Recombinantly expressed TNF-alpha in largemouth bass significantly enhanced the phagocytic ability of granulocytes/monocytes and increased their ROS activity, while also inducing apoptosis.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Microbiology
Bishnu Prasad Sinha, Priyanka Mehta, Md Asmaul Hoque, Purbita Bandopadhyay, Ayandip Nandi, Ipsita Saha, Anita Nandi Mitra, Asish Mondal, Boudhayan Bhattacharjee, Georgios Chamilos, Rajesh Pandey, Kaushik Basu, Dipyaman Ganguly
Summary: Cases of rhino-orbital mucormycosis in COVID-19 patients have been reported, but the specific immune mechanisms underlying susceptibility to this infection remain unexplored. This study aimed to investigate the regulation of circulating cytokines in COVID-19-associated mucormycosis (CAM) patients and its potential pathogenic links with myeloid phagocyte function and susceptibility to mucormycosis.
Article
Geriatrics & Gerontology
Yu Zhang, Li-hua Tang, Jia Lu, Li-ming Xu, Bao-li Cheng, Jun-yu Xiong
Summary: The study showed that ABT-263 treatment improved the survival rate of sepsis in aged mice, which was related to autophagy, and blocking autophagy could eliminate this effect. ABT-263 enhanced the phagocytic ability of peritoneal macrophages by increasing the Trem-2 receptor, and induced Beclin-1-dependent autophagy by blocking the binding of Bcl-2 to Beclin-1.
Article
Immunology
Sophia Koutsogiannaki, Lifei Hou, Toshiaki Okuno, Miho Shibamura-Fujiogi, Hongbo R. R. Luo, Koichi Yuki
Summary: Since the definition of sepsis three decades ago, extensive research has been conducted on this condition. However, there is currently no specific treatment available for sepsis, which has high rates of mortality and morbidity. This study focused on examining the role of alpha D beta 2 (CD11d/CD18) integrin in sepsis, and found that its deficiency was associated with reduced lung injury and improved outcomes. This unique role of alpha D beta 2 among the beta 2 integrin members suggests its potential as a target for improving sepsis outcomes.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medical Laboratory Technology
Anna Maria Ciaccio, Luisa Agnello, Bruna Lo Sasso, Rosaria Vincenza Giglio, Alessandro Iacona, Caterina Maria Gambino, Concetta Scazzone, Antonino Tuttolomondo, Marcello Ciaccio
Summary: Monocyte Distribution Width (MDW) is a new cell blood count parameter that measures the anisocytosis of monocytes. It has emerged as a reliable biomarker for sepsis in emergency departments and intensive care units. MDW has advantages such as low-cost and quick measurement compared to other sepsis biomarkers.
CLINICA CHIMICA ACTA
(2023)
Article
Immunology
Jing Cui, Stephanie Oehrl, Fareed Ahmad, Thorsten Brenner, Florian Uhle, Christian Nusshag, Christoph Rupp, Felix Funck, Stefan Meisel, Markus A. Weigand, Christian Morath, Knut Schaekel
Summary: This study found that the frequency of ASC-speck(+) cells in leukocytes was increased in sepsis patients compared to healthy controls, with a peak on day 1. Among 25 sepsis patients sampled for 7 days, those with lower absolute numbers of ASC-speck(+) monocytes on day 6 had a lower probability to survive, indicating the potential of using ASC-speck(+) monocytes on day 6 as a marker for identifying sepsis patients at risk of death.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Barbara Gierlikowska, Albert Stachura, Wojciech Gierlikowski, Urszula Demkow
Summary: This article provides an overview of the importance of neutrophils in sepsis, explains the dependence between cytokines and their activation, and summarizes the impact of cytokines on phagocytosis and NET formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Sarah To, Thandiwe Chavula, Mesias Pedroza, Jennifer Smith, Sandeep K. Agarwal
Summary: CDH11 plays an important role in pulmonary fibrosis by regulating macrophage development, M2 polarization, and phagocytic function.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Anugraha Gandhirajan, Sanjoy Roychowdhury, Christopher Kibler, Emily Cross, Susamma Abraham, Annett Bellar, Laura E. E. Nagy, Rachel Greenberg Scheraga, Vidula Vachharajani
Summary: Alcohol abuse is an independent risk factor for death in sepsis. SIRT2, an NAD+-dependent histone-deacetylase, suppresses phagocytosis and pathogen clearance by regulating glycolysis in ethanol-exposed macrophages. Genetic deficiency or pharmacological inhibition of SIRT2 improves bacterial clearance and survival in sepsis mice with ethanol exposure by reversing PFKP-deacetylation and restoring phagocytosis.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Manon Chauvin, Martin Larsen, Bibiana Quirant, Paul Quentric, Karim Dorgham, Luca Royer, Helene Vallet, Amelie Guihot, Behazine Combadiere, Christophe Combadiere, Jaume Barallat, Julien Mayaux, Charles-Edouard Luyt, Alexis Mathian, Zahir Amoura, Jacques Boddaert, Fernando Armestar, Guy Gorochov, Eva Martinez-Caceres, Delphine Sauce
Summary: This study found that severe and critically ill patients with SARS-CoV-2 infection exhibit exacerbated immune activation characterized by elevated neopterin blood levels. Systemic neopterin levels can serve as a biomarker of severe Covid-19, helping to identify high-risk populations.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Critical Care Medicine
Aida Meghraoui-Kheddar, Benjamin G. Chousterman, Noelline Guillou, Sierra M. Barone, Samuel Granjeaud, Helene Vallet, Aurelien Corneau, Karim Guessous, Charles de Roquetaillade, Alexandre Boissonnas, Jonathan M. Irish, Christophe Combadiere
Summary: This study aimed to identify early and specific immune signatures of sepsis severity, as well as to distinguish sepsis patients from those with noninfectious inflammatory syndrome. The results revealed two new subsets of neutrophils that are specific to sepsis and detectable through routine blood tests. This finding represents a key biological milestone that can lead to improvements in patient care.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Alexis J. Combes, Bushra Samad, Jessica Tsui, Nayvin W. Chew, Peter Yan, Gabriella C. Reeder, Divyashree Kushnoor, Alan Shen, Brittany Davidson, Andrea J. Barczak, Michael Adkisson, Austin Edwards, Mohammad Naser, Kevin C. Barry, Tristan Courau, Taymour Hammoudi, Rafael J. Arguello, Arjun Arkal Rao, Adam B. Olshen, Cathy Cai, Jenny Zhan, Katelyn C. Davis, Robin K. Kelley, Jocelyn S. Chapman, Chloe E. Atreya, Amar Patel, Adil Daud, Patrick Ha, Aaron A. Diaz, Johannes R. Kratz, Eric A. Collisson, Gabriela K. Fragiadakis, David J. Erle, Alexandre Boissonnas, Saurabh Asthana, Vincent Chan, Matthew F. Krummel
Summary: This study identifies common patterns of the immune system in different types of cancer and proposes strategies to modulate tumor immunity for improved therapy. The findings provide new insights into cancer immunity and offer a rational approach to enhance treatment.
Review
Immunology
Veronique M. Braud, Aida Meghraoui-Kheddar, Roxane Elaldi, Luciana Petti, Claire Germain, Fabienne Anjuere
Summary: The success of immune checkpoint therapy has shifted the focus to the immune system in cancer treatment design. The interaction between LLT1 and CD161 plays a crucial role in cancer development, but research is hindered by some obstacles.
FRONTIERS IN IMMUNOLOGY
(2022)
Letter
Critical Care Medicine
Romain Barthelemy, Alexandre Mebazaa, Benjamin Glenn Chousterman
INTENSIVE CARE MEDICINE
(2022)
Editorial Material
Immunology
Alexandre Boissonnas, Christophe Combadiere
Summary: The study shows that targeting the post-translational modification enzyme QPCTL can prevent the protection of CCL2 and CCL7, leading to the inactivation of their chemotactic function and limiting the recruitment of pro-tumoral macrophages.
Article
Cell Biology
Marie Laviron, Maxime Petit, Eleonore Weber-Delacroix, Alexis J. Combes, Arjun Rao Arkal, Sandrine Barthelemy, Tristan Courau, David A. Hume, Christophe Combadiere, Matthew F. Krummel, Alexandre Boissonnas
Summary: Tumor-associated macrophages (TAMs) adapt to the tumor microenvironment and exhibit different functions based on their localization in the stroma or neoplastic epithelium. Single-cell RNA sequencing and imaging techniques reveal that TAM subsets in different tissue territories and tumor lesions have distinct capacities to activate T cells and phagocytose tumor cells.
Article
Oncology
Adriana M. Mujal, Alexis J. Combes, Arjun A. Rao, Mikhail Binnewies, Bushra Samad, Jessica Tsui, Alexandre Boissonnas, Joshua L. Pollack, Rafael J. Arguello, Maxwell Meng, Sima P. Porten, Megan K. Ruhland, Kevin C. Barry, Vincent Chan, Matthew F. Krummel
Summary: In this study, the authors used single-cell RNA sequencing analysis to explore the diversity of myeloid cells in tumors and found that the transition from monocytes to macrophages is closely associated with the abundance of regulatory T cells and the quality of infiltrating T cells.
CANCER IMMUNOLOGY RESEARCH
(2022)
Article
Immunology
Amelie Guihot, Isabelle Plu, Cathia Soulie, Alice Rousseau, Cecilia Nakid-Cordero, Karim Dorgham, Christophe Parizot, Elena Litvinova, Julien Mayaux, Isabelle Malet, Paul Quentric, Behazine Combadiere, Christophe Combadiere, Olivia Bonduelle, Lucille Adam, Pierre Rosenbaum, Alexandra Beurton, Patrice Hemon, Patrice Debre, Vincent Vieillard, Brigitte Autran, Danielle Seilhean, Frederic Charlotte, Anne-Genevieve Marcelin, Guy Gorochov, Charles-Edouard Luyt
Summary: The immunopathological pulmonary mechanisms leading to Coronavirus Disease (COVID-19)-related death in adults are still poorly understood. This study found that despite strong antiviral CD4 T cell-specific responses migrating to the lung, disease severity still occurs, suggesting a potential pathogenic role for perivascular memory CD4 T cells in fatal COVID-19 pneumonia.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Pierre Bourdely, Luciana Petti, Sokchea Khou, Aida Meghraoui-Kheddar, Roxane Elaldi, Julie Cazareth, Noushine Mossadegh-Keller, Julien Boyer, Michael H. Sieweke, Gilles Poissonnet, Anne Sudaka, Veronique M. Braud, Fabienne Anjuere
Summary: This study investigated the origins, phenotypes, and functions of macrophage subsets in mouse and human skin, as well as in cutaneous squamous cell carcinoma (cSCC). The results showed that autofluorescence can serve as a functional marker for phagocytic macrophages in skin and cSCC.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Critical Care Medicine
Pierre Basse, Louis Morisson, Romain Barthelemy, Nathan Julian, Manuel Kindermans, Magalie Collet, Benjamin Huot, Etienne Gayat, Alexandre Mebazaa, Benjamin G. Chousterman
Summary: This study investigated the impact of positive pressure ventilation, central venous pressure (CVP), and inflammation on acute kidney injury (AKI) in mechanically ventilated COVID-19 patients. The results showed that high CVP levels and leukocytes count were associated with an increased risk of AKI, while PEEP levels did not appear to influence the occurrence of AKI.
ACUTE AND CRITICAL CARE
(2023)