期刊
APPLIED SCIENCES-BASEL
卷 10, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/app10093058
关键词
anti-colon cancer; apoptosis; cell cycle arrest; fucoidan; human colon carcinoma HT-29 cells; Sargassum glaucescens
类别
资金
- Yuan's General Hospital, Taiwan [YUAN-IACR-20-02]
- Ministry of Science and Technology, Taiwan [MOST 107-2320-B-992-001]
- Ministry of Education, Taiwan [MOE-RSC-108RSN0005]
Fucoidans constitute a family of fucose-rich sulfated polysaccharides, which possess multiple characteristics, including antioxidant, antitumor, antivirus, anticoagulant, and anti-inflammatory properties. In addition, the incidence of colon cancer has risen rapidly worldwide. In the present study, fucoidan extracts were extracted from the Sargassumglaucescens (SG) pretreated by compressional-puffing, and four fucoidans (SG1-SG4) were obtained with different puffing conditions. It was found that SG4 possessed the highest extraction yield, relatively high cytotoxicity against human colon carcinoma HT-29 cells, and relatively low cytotoxicity to normal cells, as compared to the other extracted fucoidans. Moreover, SG4 caused cell cycle arrest of HT-29 cells at sub-G(1), S, and G(2)/M phases. SG4 also induced HT-29 cellular apoptosis, as evidenced by the loss of mitochondrial membrane potential (MMP), increased cytochrome c release, activation of caspase-9 and -3, increased DNA fragmentation, and increased early and late apoptotic cells visualized by annexin V/propidium iodide (PI) assay. Additional biological experiments revealed that the Akt/mammalian target of rapamycin (mTOR)/S6 pathway is involved in SG4-induced apoptosis of HT-29 cells. These results clearly indicate that SG4 showed anti-colon cancer potential via the induction of cell cycle arrest and apoptosis, and thus may have a possible application as an adjuvant therapeutic agent in colon cancer treatment.
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