Article
Agricultural Engineering
Thomas O. Butler, Gloria Padmaperuma, Alessandro M. Lizzul, Joe McDonald, Seetharaman Vaidyanathan
Summary: The study found that using the commercial powdered media Cell-Hi JWP for the cultivation of P. tricornutum outdoors in the UK yielded good performance characteristics and cost-effectiveness. There was a strong positive correlation between fucoxanthin and chlorophyll a content, and a weak inverse correlation between EPA content and temperature. Commensal bacterial counts showed a sinusoidal growth profile with a transition in community dominance observed.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Andrew R. Burch, Cody W. Yothers, Michelle R. Salemi, Brett S. Phinney, Pramod Pandey, Annaliese K. Franz
Summary: This study found that diatom Phaeodactylum tricornutum cultivated on flushed dairy manure wastewater produces more polyunsaturated fatty acids and that biomass and lipid yields are influenced by the nitrogen content in the wastewater dilution. Proteomic analysis also revealed differences in protein regulation in Phaeodactylum tricornutum cultivated on wastewater compared to synthetic seawater, providing insights into how the proteome of this algae reorganizes in response to complex wastewater sources.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Xiaotian Han, Ting Zhao, Liyan He, Yanfen Jia, Ren-cheng Yu
Summary: Environmental factors, especially temperature and light, play a crucial role in the composition of lipid, protein, and carbohydrate in microalgae. FTIR spectrometry was used to detect biomacromolecules in Phaeodactylum tricornutum cells at different temperatures and illumination conditions. The results showed different patterns in biomacromolecule content at different temperatures, with 20°C being unique. The highest contents of biomacromolecules were observed at 2000 lx, while increasing illumination led to a decrease in content. The study demonstrated the potential of FTIR as a new technique for studying metabolic mechanisms.
Article
Engineering, Chemical
Thomas O. Butler, Karen Acurio, Joy Mukherjee, Miriam M. Dangasuk, Omar Corona, Seetharaman Vaidyanathan
Summary: The study compared various bio-based and traditional chemical flocculants for harvesting diatoms and found that Tanac's tannin-based Tanfloc 8025 was the most promising, with low cost, significant effectiveness, and the ability to operate effectively under a wide range of conditions.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Chemistry, Applied
Rodolphe Dumontier, Corinne Loutelier-Bourhis, Marie-Laure Walet-Balieu, Carole Burel, Alain Mareck, Carlos Afonso, Patrice Lerouge, Muriel Bardor
Summary: Microalgae, such as diatoms, are emerging as production systems for recombinant proteins like monoclonal antibodies. Efficient analytical methodologies are required to characterize the N-glycosylation machinery of the host cell and the glycoprotein-based bio-pharmaceuticals produced by microalgae. This study found that the diatom Phaeodactylum tricornutum can synthesize human-like oligomannosides, making it an environmentally friendly and advantageous expression system for producing biopharmaceuticals.
CARBOHYDRATE POLYMERS
(2021)
Article
Biotechnology & Applied Microbiology
Wenjia Gu, John M. Kavanagh, Dale D. McClure
Summary: A kinetic model for EPA-producing microalga Phaeodactylum tricornutum was developed and successfully validated, demonstrating its potential for large-scale EPA production.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Chaitanya Kumar Reddy Pocha, Wen Yi Chia, Kit Wayne Chew, Heli Siti Halimatul Munawaroh, Pau Loke Show
Summary: This paper discusses the extraction of fucoxanthin, focusing on Phaeodactylum tricornutum and briefly mentioning Chaetoceros calcitrans and Isochrysis galbana. The cultivation, harvesting, and drying processes of these diatoms are briefly discussed, followed by an introduction to traditional and new extraction methods. The limitations of current extraction strategies and the potential for cost-effective technologies are also discussed.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Multidisciplinary Sciences
Ananya Agarwal, Orly Levitan, Helena Cruz de Carvalho, Paul G. Falkowski
Summary: Unlike most plants, unicellular algae can adapt to changes in irradiance within a few hours to days through an enigmatic signaling pathway that coordinates changes in plastid and nuclear gene expression. Functional studies on the model diatom, Phaeodactylum tricornutum, identified two potential signal transduction molecules responsible for photoacclimation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
A-Hyeon Lee, Hye-Yoon Shin, Jong-Hwi Park, Song Yi Koo, Sang Min Kim, Seung-Hoon Yang
Summary: Pro-inflammatory cytokines are produced and secreted through the activation of NF-kappa B and NLRP3 inflammasome signaling pathways. Fucoxanthin, found in Phaeodactylum tricornutum extracts, has been shown to inhibit the activation of these pathways and decrease the production of major pro-inflammatory cytokines.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Tomas Grivalsky, Antonin Strizek, Pavel Pribyl, Jaromir Lukavsky, Radim Cegan, Roman Hobza, Pavel Hrouzek
Summary: Detection of fast-growing green algae Chlorella vulgaris as a contaminant in diatom Phaeodactylum tricornutum cultures using various approaches reveals PCR/qPCR as the most reliable and sensitive method with a detection sensitivity close to 75 cells/mL. This method is effective even in mixed cultures containing a large number of P. tricornutum cells. The cultivation media exchange method from sea water to fresh water is similarly sensitive to PCR approaches for detecting contaminants, albeit with a longer detection time.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Coralie Chuberre, Philippe Chan, Marie-Laure Walet-Balieu, Francois Thiebert, Carole Burel, Julie Hardouin, Bruno Gugi, Muriel Bardor
Summary: This study conducted a comparative proteomic analysis of three different morphotypes of Phaeodactylum tricornutum. The results revealed significant differences in certain biological processes between oval and triradiate cells compared to fusiform cells, and the oval cells showed a distinct metabolism.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Xue Zhao, Achal Rastogi, Anne Flore Deton Cabanillas, Ouardia Ait Mohamed, Catherine Cantrel, Berangere Lombard, Omer Murik, Auguste Genovesio, Chris Bowler, Daniel Bouyer, Damarys Loew, Xin Lin, Alaguraj Veluchamy, Fabio Rocha Jimenez Vieira, Leila Tirichine
Summary: The PRC2 complex plays a crucial role in gene silencing and cell development in multicellular organisms, and recent studies have shown its existence and importance in unicellular species, particularly in microalgae. This discovery emphasizes the significance of PRC2 in cell differentiation in unicellular organisms, extending its ancestral function beyond current understanding of its evolutionary context.
Article
Microbiology
Bing Li, Di Wu, Yan Li, Yan Shi, Chenlin Wang, Jiasi Sun, Chunfeng Song
Summary: Antibiotic resistance is a significant environmental challenge, and the use of microalgae for environmental purification shows great potential, particularly in the degradation of sulfadimethoxine.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Environmental Sciences
Paula Santiago-Diaz, Argimiro Rivero, Milagros Rico, Aridane Gonzalez Gonzalez, Melchor Gonzalez-Davila, Magdalena Santana-Casiano
Summary: This study focuses on the effect of lethal and sub-lethal copper concentrations on the production of free amino acids and polyphenols in the marine diatom Phaeodactylum tricornutum. The results show that under lethal concentrations of copper, there is a significant increase in the production of free amino acids and polyphenols, indicating their role in protective mechanisms against copper toxicity.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Daniela Couto, Tania Melo, Tiago A. Conde, Margarida Costa, Joana Silva, M. Rosario M. Domingues, Pedro Domingues
Summary: This study characterized the polar lipidome of Chlorella vulgaris grown under autotrophic and heterotrophic conditions, revealing differences in lipid species and abundance distribution. Heterotrophic cultures had a higher abundance of low unsaturated species, while autotrophic cultures were rich in highly unsaturated glycolipids and lyso species. The fatty acids profile showed a high content of omega-3 polyunsaturated fatty acids in autotrophic cultures and omega-6 fatty acids in heterotrophic cultures. The two lipid extracts exhibited high antioxidant activity and inhibitory capacity for cyclooxygenase-2, providing evidence of polar lipid plasticity in microalgae for biotechnology applications.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Review
Plant Sciences
Isabel Desgagne-Penix
Summary: Amaryllidaceae alkaloids are a group of specialized metabolites primarily found in the Amaryllidaceae plant family, with over 600 naturally occurring varieties identified, including galanthamine used to treat neurodegenerative disorders like Alzheimer's disease. Limited commercial availability hinders their clinical development despite promising pharmacological potential. There have been significant advancements in understanding the biosynthesis of Amaryllidaceae alkaloids in recent years through comparative transcriptomic and biochemical approaches.
PHYTOCHEMISTRY REVIEWS
(2021)
Review
Genetics & Heredity
Adesola J. Tola, Amal Jaballi, Hugo Germain, Tagnon D. Missihoun
Summary: ALDH enzymes in plants play crucial roles in metabolism, signaling, and development by metabolizing various aldehyde molecules. Recent research has shed light on new roles of these enzymes, prompting further exploration of their potential in biotechnology. Future investigations should focus on uncovering more about these enzymes to enhance our understanding of gene signaling and plant development.
Article
Microbiology
Karen Cristine Goncalves dos Santos, Gervais Pelletier, Armand Seguin, Francois Guillemette, Jeffrey Hawkes, Isabel Desgagne-Penix, Hugo Germain
Summary: The study revealed that transgenic plants expressing candidate effectors of poplar rust fungus Mlp caused significant gene and metabolite deregulation. Genes involved in pattern-triggered immunity were down-regulated, and similar effector sequences led to correlated patterns of gene and metabolite deregulation. This highlights the importance of investigating individual effectors rather than generalizing based on sequence similarity when studying plant susceptibility.
Article
Microbiology
Md. Saifur Rahman, Mst Hur Madina, Melodie B. Plourde, Karen Cristine Goncalves dos Santos, Xiaoqiang Huang, Yang Zhang, Jean-Francois Laliberte, Hugo Germain
Summary: The study found that the effector Mlp37347 mainly accumulates at plasmodesmata during infection, promoting parasitic growth by increasing plasmodesmatal flux and reducing callose deposition.
Article
Plant Sciences
Laurence Tousignant, Aracely Maribel Diaz-Garza, Bharat Bhusan Majhi, Sarah-Eve Gelinas, Aparna Singh, Isabel Desgagne-Penix
Summary: Transcriptome analysis of Leucojum aestivum identified 50 key genes associated with Amaryllidaceae alkaloid biosynthesis, including the cytosolic localized norbelladine synthase. The study provides important information on the biosynthesis of Amaryllidaceae alkaloids and offers potential for bioengineering applications.
Article
Food Science & Technology
Marco Masi, Roberta Di Lecce, Natacha Merindol, Marie-Pierre Girard, Lionel Berthoux, Isabel Desgagne-Penix, Viola Calabro, Antonio Evidente
Summary: Ten Amaryllidaceae alkaloids were isolated for the first time from Pancratium maritimum collected in Calabria region, Italy. Some of these compounds showed cytotoxic and antiviral activity, inhibiting human immunodeficiency virus (HIV) and dengue virus (DENV).
Review
Biochemistry & Molecular Biology
Manoj Koirala, Vahid Karimzadegan, Nuwan Sameera Liyanage, Natacha Merindol, Isabel Desgagne-Penix
Summary: Amaryllidaceae alkaloids (AAs) are therapeutic plant metabolites exclusively produced by the Amaryllidaceae plant family. The production variability of AAs in plants hinders their pharmaceutical applications. Biotechnological approaches such as in vitro culture and synthetic biology are being developed to enhance the production of these plant-derived drugs.
Article
Biochemistry & Molecular Biology
Marie-Pierre Girard, Vahid Karimzadegan, Marianne Heneault, Francis Cloutier, Gervais Berube, Lionel Berthoux, Natacha Merindol, Isabel Desgagne-Penix
Summary: Amaryllidaceae alkaloids, particularly norbelladine and its derivatives, show therapeutic potential with antiviral and anticholinesterase properties. O-methylated norbelladine-type alkaloids possess inhibitory effects on DENV infection and butyrylcholinesterase.
Article
Plant Sciences
A. Diamond, S. Barnabe, I Desgagne-Penix
Summary: This article discusses the findings regarding the cellular localization and activity of enzymes involved in vanillin biosynthesis, as well as the controversies arising from these findings. This helps to further understand the pathway of vanillin biosynthesis and emphasizes the need for additional research to resolve the debate.
Article
Plant Sciences
Roberta Di Lecce, Natacha Merindol, Mayra Galarza Perez, Vahid Karimzadegan, Lionel Berthoux, Angela Boari, Christian Zidorn, Maurizio Vurro, Giuseppe Surico, Isabel Desgagne-Penix, Antonio Evidente
Summary: Major threats to human lifespan include various diseases and the impact of climate change on agricultural productivity. Therefore, the search for new biopesticides and medicines becomes increasingly important. The plants on Lampedusa Island contain a variety of metabolites that have potential applications in agriculture and medicine.
Article
Biochemistry & Molecular Biology
Zainab Fakih, Melodie B. Plourde, Charlene Eugenie Tomi Nkouankou, Victor Fourcassie, Sylvie Bourassa, Arnaud Droit, Hugo Germain
Summary: Plants have developed strategies to cope with various stresses, and ribosomes can adapt to different stress conditions. By analyzing immunopurified ribosomes from Arabidopsis leaves treated with an inducer of plant innate immunity, potential ribosome-associated proteins involved in innate immunity were identified.
PLANT MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zainab Fakih, Melodie B. Plourde, Hugo Germain
Summary: Plants have developed strategies to adapt to stress through regulating the translation process. Recent research has shown that ribosomes can remodel to adapt to stresses. Whether some ribosomal proteins enable ribosomes to preferentially translate specific mRNAs remains to be studied. This study found that silencing certain ribosomal proteins can affect the translation of specific genes.
Article
Biochemistry & Molecular Biology
Manoj Koirala, Karen Cristine Gncalves dos Santos, Sarah-Eve Gelinas, Simon Ricard, Vahid Karimzadegan, Basanta Lamichhane, Nuwan Sameera Liyanage, Natacha Merindol, Isabel Desgagn
Summary: This study demonstrates for the first time that the combination of auxin and light significantly affects the growth, survival, and morphogenesis of C. x powellii 'Album' in vitro tissues. Furthermore, this combination upregulates the expression of alkaloid biosynthetic genes and increases the content of certain alkaloids, thereby enhancing the defense and therapeutic potential of the calli.
Article
Biochemical Research Methods
Remy Beauchemin, Natacha Merindol, Elisa Fantino, Pamela Lavoie, Serge Basile Nouemssi, Fatma Meddeb-Mouelhi, Isabel Desgagne-Penix
Summary: Chlamydomonas reinhardtii has been genetically engineered for heterologous protein expression, but its ability to silence transgene expression through epigenetic changes hinders its further application. In this study, researchers discovered that treatment with HDAC inhibitors can revert transgene silencing and successfully trigger the expression of heterologous proteins in C. reinhardtii.
BIOTECHNOLOGY JOURNAL
(2023)
