Article
Polymer Science
Samr Kassem, Stanislav S. Piletsky, Hasan Yesilkaya, Ozcan Gazioglu, Medhanie Habtom, Francesco Canfarotta, Elena Piletska, Alan C. Spivey, Eric O. Aboagye, Sergey A. Piletsky
Summary: This study demonstrated the biodistribution, clearance, and immunogenicity of solid-phase synthesized nanoMIPs in a rat model, showing their ability to cross the blood-brain barrier and be cleared from the body via feces and urine. With their low cytotoxicity and ability to access various tissue types, these results pave the way for in vivo applications of nanoMIPs.
Article
Chemistry, Multidisciplinary
Cassandra E. Callmann, Matthew K. Vasher, Anindita Das, Caroline D. Kusmierz, Chad A. Mirkin
Summary: The biological properties of spherical nucleic acids (SNAs) are primarily determined by the oligonucleotide surface density, rather than the nanoparticle core identity. Furthermore, the payload-to-carrier mass ratio of SNAs is inversely proportional to the core size. Ultrasmall nanoparticle constructs (<10 nm diameter) exhibit unique properties, including increased payload-to-carrier ratios, reduced liver accumulation, renal clearance, and enhanced tumor infiltration.
Article
Biochemistry & Molecular Biology
Silvia Voci, Cristina Pangua, Maria Cristina Martinez-Oharriz, Paula Aranaz, Maria Collantes, Juan M. Irache, Donato Cosco
Summary: The study characterizes Brij O2-stabilized gliadin nanoparticles for potential oral drug delivery. Physico-chemical evaluations and in vivo studies in rats and Caenorhabditis elegans confirmed the nanoparticles' ability to accumulate in the mucosa and move along the digestive system. The nanoparticles showed antioxidant activity, decreased age-related lipofuscin pigment, and demonstrated a safe profile. These findings support the future application of these nanoparticles for oral delivery of bioactives.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biotechnology & Applied Microbiology
Rita M. Pinto, Saleh Yazdani, Catarina Leal Seabra, Martine De Jonge, Mukaddes Izci, Rebeca Cruz, Susana Casal, Stefaan J. Soenen, Salette Reis, Claudia Nunes, Patrick Van Dijck
Summary: Staphylococcus aureus is a high priority pathogen due to its prevalence and ability to form biofilms. Current treatments do not target the matrix that contributes to antimicrobial tolerance. This study proposes the use of lipid nanoparticles encapsulating caspofungin to disrupt the matrix, and combining them with moxifloxacin-loaded nanoparticles to reduce biofilms. In vitro and in vivo studies showed promising results, with reduced biofilm formation and no bacterial dissemination into vital organs.
NPJ BIOFILMS AND MICROBIOMES
(2023)
Review
Pharmacology & Pharmacy
Abraham J. Domb, Ghorbanali Sharifzadeh, Victoria Nahum, Hossein Hosseinkhani
Summary: Nanomaterials are widely used in biomedical applications, but concerns have been raised about their potential risks to tissues, cells, and organs. Understanding the underlying mechanisms of nanotoxicity and conducting in vitro and in vivo experiments are crucial for assessing their toxicity. Physicochemical properties of nanomaterials play a significant role in determining their toxicity.
Review
Pharmacology & Pharmacy
Noorjahan Aibani, Raj Rai, Parth Patel, Grace Cuddihy, Ellen K. Wasan
Summary: Chitosan, with its unique properties, is a valuable choice for nanoparticulate drug delivery applications, but its interactions at cellular and systemic levels require further study. The positive charge of chitosan allows for efficient attachment to cells, enhancing cellular uptake.
Article
Nanoscience & Nanotechnology
Julia Nowak-Jary, Beata Machnicka
Summary: Magnetic iron oxide nanoparticles have broad potential applications in medical and biological sciences. They can be used in targeted drug delivery, internal radiotherapy, magnetic resonance imaging, hyperthermia, and radiolabelling. The appropriate design of nanoparticles allows them to be delivered to desired tissues and organs, with an external magnetic field increasing their biodistribution. Factors such as size, shape, surface charge, coating molecules, and route of administration affect their biodistribution. Modern techniques can determine nanoparticle accumulation sites and concentration in isolated tissues or the body in vivo.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Biochemical Research Methods
Mallaiah Devanabanda, Siva Sankar Sana, Ramakrishna Vadde, Ramanadham Madduri, Raja Venkatesan, Gaber E. Eldesoky, Seong-Cheol Kim
Summary: The distribution and accumulation of labeled nanoparticles and silver nanoparticles in tissues after oral administration were studied. Rhodamine-labeled bovine serum albumin and chitosan were used to coat gold nanoparticles and silver nanoparticles. The results showed that these coated nanoparticles had significant accumulation in the liver, kidney, and thymus.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Multidisciplinary Sciences
Chae Moon Hong, Prakash Gangadaran, Ji Min Oh, Ramya Lakshmi Rajendran, Arunnehru Gopal, Liya Zhu, Byeong-Cheol Ahn
Summary: This study developed a nuclear imaging method for tracking EVs derived from cell lines, showing that radioiodine-labeled EVs had good biodistribution. The radioiodine labeling did not significantly affect the morphology and properties of EVs.
SCIENTIFIC REPORTS
(2021)
Article
Pharmacology & Pharmacy
Umay Merve Guven, Ebru Basaran
Summary: In this study, Olopatadine hydrochloride was successfully loaded into chitosan nanoparticles for the treatment of ocular allergy. The nanoparticles showed prolonged corneal residence time in vivo, potentially enhancing the therapeutic efficacy for ocular allergy treatment.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2021)
Article
Polymer Science
Ishaq Sider, Mahmoud M. A. Nassar
Summary: This study explores the use of olive pit flour to reinforce recycled low-density polyethylene, focusing on improving the compatibility between bio-filler and polymer through chemical treatment. The results show that composites obtained with alkali-treated olive pits exhibit better properties, indicating potential for developing green composites.
Article
Chemistry, Applied
Jun Wang, Justin Liu, Dao-Qiang Lu, Lijing Chen, Rujia Yang, Dahai Liu, Bin Zhang
Summary: In this study, diselenide-crosslinked nanoparticles were successfully fabricated for doxorubicin delivery. The nanoparticles exhibited desirable morphology and size distribution, and the starting material used improved biocompatibility and physiological stability. The high drug encapsulation efficiency of the nanoparticles was achieved through electrostatic interactions between doxorubicin and the material. In vitro and in vivo experiments demonstrated that the doxorubicin-loaded diselenide-crosslinked nanoparticles had higher drug accumulation and enhanced growth inhibition compared to the control samples.
CARBOHYDRATE POLYMERS
(2022)
Article
Nanoscience & Nanotechnology
Jie Qiao, Jun Yang, Saman Jalili
Summary: In this study, propolis-loaded chitosan nanoparticles were developed as a potential treatment for gastrointestinal infections. The nanoparticles showed non-toxicity towards intestinal epithelial cells and exhibited higher antibacterial and anti-inflammatory activities compared to propolis-free chitosan nanoparticles. In vivo experiments demonstrated that the nanoparticles had a comparable anti-diarrhea function to the standard drug, norfloxacin, and also increased the number of beneficial bacteria while reducing inflammation in the colon tissue.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Madeline B. Gelb, Kathryn M. M. Messina, Daniele Vinciguerra, Jeong Hoon Ko, Jeffrey Collins, Mikayla Tamboline, Shili Xu, F. Javier Ibarrondo, Heather D. Maynard
Summary: This study investigates the safety and stabilizing mechanism of poly(trehalose methacrylate) (pTrMA) as an excipient for insulin. The results show that pTrMA does not elicit an immune response or cytokine production. Imaging studies reveal that pTrMA is rapidly excreted from the body and has minimal liver accumulation. Moreover, pTrMA does not affect the plasma lifetime of insulin. Adding pTrMA improves the stability of insulin under standard storage conditions, inhibiting deamidation and fibrillation of the peptide.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Biochemistry & Molecular Biology
Anne Strohbach, Raila Busch
Summary: The therapeutic efficacy of cardiovascular devices post-implantation relies heavily on host-initiated complement and coagulation cascades, with understanding these responses being crucial for newly developed biomaterials. However, challenges in producing truly blood-compatible materials may stem from a lack of understanding of thrombosis and inflammation mechanisms. In vitro models can provide valuable insights into biomaterial-induced complement activation and coagulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Virology
Vladimir Marchenko, Irina Zelinskaya, Yana Toropova, Tatyana Shmakova, Ekaterina Podyacheva, Dmitry Lioznov, Irina N. Zhilinskaya
Summary: The study identified histological changes and alterations in the functional activity of pulmonary and mesenteric blood vessels in Wistar rats infected with influenza virus. The results showed development of endothelial dysfunction in both arterial systems, with pulmonary arteries exhibiting increased response to vasodilators and decreased response to vasoconstrictors, while mesenteric arteries displayed the opposite pattern, indicating a complex impact of the virus on the cardiovascular system.
Article
Chemistry, Physical
Mojde Moradi Pour, Roohallah Saberi Riseh, Yury A. Skorik
Summary: This research proposes a new formulation that uses nanocomposites to encapsulate beneficial bacteria for synergistic control of Phytophthora drechsleri.
Article
Biochemistry & Molecular Biology
Natallia V. Dubashynskaya, Anton N. Bokatyi, Ekaterina R. Gasilova, Anatoliy V. Dobrodumov, Yaroslav A. Dubrovskii, Elena S. Knyazeva, Yuliya A. Nashchekina, Elena V. Demyanova, Yury A. Skorik
Summary: The development of nanotechnology-based antibiotic delivery systems is crucial for combating microbial multidrug resistance. This study successfully synthesized conjugates of colistin and hyaluronic acid, which showed improved biopharmaceutical characteristics and reduced cytotoxicity. These conjugates have great potential for the development of safe and effective nanodrugs for antimicrobial therapy.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Valentina A. Petrova, Natallia V. Dubashynskaya, Iosif V. Gofman, Alexey S. Golovkin, Alexander I. Mishanin, Arthur D. Aquino, Daria V. Mukhametdinova, Alexandra L. Nikolaeva, Elena M. Ivan'kova, Alexander E. Baranchikov, Alexander V. Yakimansky, Vladimir K. Ivanov, Yury A. Skorik
Summary: Polymeric nanocomposite materials have the potential to enhance tissue-engineered scaffolds by influencing the structure and properties of polymeric materials and regulating cell behavior. In this study, cerium oxide nanoparticles were incorporated into a chitosan film to promote the proliferation of mesenchymal stem cells. The resulting CS-CeONP films exhibited good biocompatibility and regenerative potential in both in vitro and in vivo experiments.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Natallia V. V. Dubashynskaya, Anton N. N. Bokatyi, Anatoliy V. V. Dobrodumov, Igor V. V. Kudryavtsev, Andrey S. S. Trulioff, Artem A. A. Rubinstein, Arthur D. D. Aquino, Yaroslav A. A. Dubrovskii, Elena S. S. Knyazeva, Elena V. V. Demyanova, Yuliya A. A. Nashchekina, Yury A. A. Skorik
Summary: The growth of microbial multidrug resistance is a problem in modern clinical medicine. Chemical modification of active pharmaceutical ingredients is an attractive strategy to improve their biopharmaceutical properties. Conjugation of antimicrobial drugs with natural polysaccharides provides targeted delivery, controlled drug release, and reduced toxicity. The study synthesized colistin conjugates with succinyl chitosan and found promising results in terms of drug release, antimicrobial activity, and reduction of drug nephrotoxicity and inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Natallia V. V. Dubashynskaya, Ekaterina R. R. Gasilova, Yury A. A. Skorik
Summary: The marine polysaccharide fucoidan (FUC) shows great potential in pharmaceutical research for developing drug delivery systems with modified release and targeted delivery. The presence of a sulfate group in FUC makes it suitable for forming interpolyelectrolyte complexes (PECs) with various polycations. However, the structural diversity of FUC poses challenges in designing FUC-based nanoformulations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Nataliia V. Katolikova, Aleksandr A. Khudiakov, Daria D. Shafranskaya, Andrey D. Prjibelski, Alexey E. Masharskiy, Mikael S. Mor, Alexey S. Golovkin, Anastasia K. Zaytseva, Irina E. Neganova, Evgeniya V. Efimova, Raul R. Gainetdinov, Anna B. Malashicheva
Summary: Elaboration of protocols to differentiate human pluripotent stem cells to dopamine neurons is important for Parkinson's disease cell replacement therapy. Notch signaling plays a role in the differentiation process, as its activation led to changes in gene expression specific to caudal parts of the brain and a decrease in genes important for axon and dendrite formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Polina Klauzen, Daria Semenova, Daria Kostina, Vladimir Uspenskiy, Anna Malashicheva
Summary: Research has found that there are significant differences between stenotic aortic valve interstitial cells and normal cells under osteogenic stimulation, and the purinergic signaling of stenotic cells is impaired, suggesting its potential as a target for anti-calcification therapy.
Article
Biochemistry & Molecular Biology
Valentina A. A. Petrova, Iosif V. V. Gofman, Natallia V. V. Dubashynskaya, Alexey S. S. Golovkin, Alexander I. I. Mishanin, Elena M. M. Ivan'kova, Dmitry P. P. Romanov, Albert K. K. Khripunov, Elena N. N. Vlasova, Alexandra V. V. Migunova, Alexander E. E. Baranchikov, Vladimir K. K. Ivanov, Alexander V. V. Yakimansky, Yury A. A. Skorik
Summary: New composite films were prepared by incorporating bacterial cellulose nanofibers and cerium oxide nanoparticles into chitosan matrices. The amount of nanofillers affected the structure and properties of the composites, including film stiffness and strength. Increasing the concentration of nanoceria improved the biocompatibility and adhesion of the films to mesenchymal stem cells. These nanocomposite films exhibit favorable properties for use as matrix materials in cell culture and wound dressings.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Vladimir Marchenko, Irina Zelinskaya, Yana Toropova, Ekaterina Podyacheva, Mikhail Martynov, Daria Mukhametdinova, Dmitry Lioznov, Irina N. Zhilinskaya
Summary: This study aimed to assess the functional activity of mesenteric blood vessels in rats with acute cardiomyopathy infected with Influenza A(H1N1)pdm09 virus. The results showed that infection with the virus led to a significant decrease in vascular activity, alteration in expression of endothelial factors, and changes in plasma concentrations of PAI-1 and tPA.
Article
Polymer Science
Natallia V. V. Dubashynskaya, Valentina A. A. Petrova, Andrey V. V. Sgibnev, Vladimir Y. Y. Elokhovskiy, Yuliya I. I. Cherkasova, Yury A. A. Skorik
Summary: The development of cryogels containing metronidazole (MET) based on carrageenan (CRG) and partially deacetylated chitin nanowhiskers (CNWs) has shown promise for improved vaginal delivery. The incorporation of CNWs significantly improved the strength and homogeneity of the cryogels, resulting in sustained drug release within 24 hours. The mechanism of prolonged release was related to polymer swelling and chain relaxation in the matrix, as demonstrated by the Korsmeyer-Peppas and Peppas-Sahlin models. These cryogels exhibited a prolonged antiprotozoal effect, making them potential dosage forms for the treatment of vaginal infections.
Article
Pharmacology & Pharmacy
Arsalan D. Badaraev, Marat I. Lerner, Olga V. Bakina, Dmitrii V. Sidelev, Tuan-Hoang Tran, Maksim G. Krinitcyn, Anna B. Malashicheva, Elena G. Cherempey, Galina B. Slepchenko, Anna I. Kozelskaya, Sven Rutkowski, Sergei I. Tverdokhlebov
Summary: Biocompatible poly(lactide-co-glycolide) scaffolds fabricated via electrospinning are modified on the surface with copper and titanium to improve antibacterial properties without toxicity to mouse fibroblasts. Different amounts of copper and titanium are achieved by changing the magnetron sputtering process parameters. The scaffold sample with the highest copper to titanium ratio exhibits the best antibacterial properties and no toxicity to mouse fibroblasts, although it has a toxic effect on human gingival fibroblasts. The optimal scaffold sample is surface-modified with a medium ratio of copper and titanium.
Article
Chemistry, Analytical
Tatiana Zimina, Nikita Sitkov, Vladimir Karasev, Yury Skorik, Alexey Kolobov, Alexander Kolobov, Nikolay Bunenkov, Viktor Luchinin
Summary: The study used in silico modelling to simulate peptides complementary to lactoferrin, specifically focusing on the bonding site between pneumococcal surface protein (PSP) and lactoferrin (LF). The proposed peptide, EEVAPQAQAKIAELENQVHRLE, was synthesized and analyzed to confirm its purity. Experimental results showed that the peptide formed a complex with LF, leading to a change in mobility. However, no binding was observed with homomyeloperoxidase and myoglobin.
Article
Biochemistry & Molecular Biology
Natallia V. Dubashynskaya, Anton N. Bokatyi, Tatiana S. Sall, Tatiana S. Egorova, Yuliya A. Nashchekina, Yaroslav A. Dubrovskii, Ekaterina A. Murashko, Elena N. Vlasova, Elena V. Demyanova, Yury A. Skorik
Summary: Polymeric drug delivery systems enhance the properties of antibiotics by increasing bioavailability, providing controlled-release, and reducing toxicity. This study describes the modification of conjugates based on colistin and hyaluronic acid with vitamin B12, which improved intestinal permeability, achieved slow release, and retained antimicrobial activity. These vitamin B12-modified conjugates may be promising oral delivery systems with improved biopharmaceutical properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Natallia V. Dubashynskaya, Anton N. Bokatyi, Andrey S. Trulioff, Artem A. Rubinstein, Igor V. Kudryavtsev, Yury A. Skorik
Summary: This study aimed to develop a dexamethasone phosphate delivery system based on hyaluronic acid and a cationic chitosan derivative, which showed both excellent mucoadhesion and prolonged drug release.