期刊
FRONTIERS IN PHARMACOLOGY
卷 11, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fphar.2020.00499
关键词
rheumatoid arthritis; quercetin; human umbilical cord mesenchymal stem cell; Toll-like receptor 3; interleukin 6; indoleamine 2; 3-dioxygenase
资金
- Special Funding for TCM Science and Technology Research of Guangdong Provincial Hospital of Chinese Medicine [CMR-20170220-1001, YN2016QJ02]
- Traditional Chinese Medicine Bureau Foundation of Guangdong Province [20183005]
The beneficial effect of quercetin in rheumatic diseases is unclear. Studies have already confirmed that human umbilical cord mesenchymal stem cells (hUCMSCs) alleviate some symptoms of rheumatoid arthritis (RA) by their immunosuppressive capacities. This study explored whether there are additive effects of quercetin and hUCMSCs on peripheral blood mononuclear cells (PBMCs) under simulated rheumatic conditions. hUCMSCs were pretreated with quercetin (10 mu M) before coculture with TNF-alpha/IFN-gamma-stimulated PBMCs at a ratio of 1:1 for 3 days. PBMC proliferation was inhibited, and the proportion of Th17 cells was shifted. These effects may be related to the effect of quercetin on functional molecules in hUCMSCs, including nitric oxide (NO), indoleamine 2,3-dioxygenase (IDO), interleukin 6 (IL-6) and Toll-like receptor-3 (TLR-3) and the Akt/I kappa B pathways. These results suggest that quercetin effectively promoted the immunoregulatory effect of hUCMSCs by inhibiting the Akt/I kappa B pathway, activating the Toll-like receptor-3 pathway, and regulating downstream cytokines.
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