期刊
CHEMPHYSCHEM
卷 18, 期 1, 页码 72-78出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cphc.201601062
关键词
label-free imaging; light sheet fluorescence microscopy; partial least squares-discriminant analysis; piriformospora indica; surface-enhanced Raman spectroscopy
资金
- American Cancer Society
- JHU Whiting School of Engineering Startup Funds
Imaging tip growth in fungal hyphae is highly warranted to unravel the molecular mechanism of this extraordinarily precise and localized phenomenon. In situ probing of fungal cultures, however, have been challenging due to their inherent complexity and light penetration issues associated with conventional optical imaging. In this work, we report a label-free approach using a combination of light sheet microscopy and Raman spectroscopy to obtain concomitant morphological and biochemical information from the growing specimen. We show that the variance in morphology in the symbiotic fungus Piriformospora indica are rooted in the underlying differences in chemical composition in the specific growth zones. Our findings suggest that this potent two-pronged approach can comprehensively characterize growth areas and elucidate microbe interactions in still developing colonies with high sensitivity and multiplexing capability.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据